Development of RNAi Methods for Peregrinus maidis, the Corn Planthopper
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| Publicado en: | PLoS One vol. 8, no. 8 (Aug 2013), p. e70243 |
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| Autor Principal: | |
| Outros autores: | , , , |
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Public Library of Science
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| Acceso en liña: | Citation/Abstract Full Text Full Text - PDF |
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| 001 | 1430411749 | ||
| 003 | UK-CbPIL | ||
| 022 | |a 1932-6203 | ||
| 024 | 7 | |a 10.1371/journal.pone.0070243 |2 doi | |
| 035 | |a 1430411749 | ||
| 045 | 2 | |b d20130801 |b d20130831 | |
| 084 | |a 174835 |2 nlm | ||
| 100 | 1 | |a Yao, Jianxiu | |
| 245 | 1 | |a Development of RNAi Methods for Peregrinus maidis, the Corn Planthopper | |
| 260 | |b Public Library of Science |c Aug 2013 | ||
| 513 | |a Journal Article | ||
| 520 | 3 | |a The corn planthopper, Peregrinus maidis, is a major pest of agronomically-important crops. Peregrinus maidis has a large geographical distribution and transmits Maize mosaic rhabdovirus (MMV) and Maize stripe tenuivirus (MSpV). The objective of this study was to develop effective RNAi methods for P. maidis. Vacuolar-ATPase (V-ATPase) is an essential enzyme for hydrolysis of ATP and for transport of protons out of cells thereby maintaining membrane ion balance, and it has been demonstrated to be an efficacious target for RNAi in other insects. In this study, two genes encoding subunits of P. maidis V-ATPase (V-ATPase B and V-ATPase D) were chosen as RNAi target genes. The open reading frames of V-ATPase B and D were generated and used for constructing dsRNA fragments. Experiments were conducted using oral delivery and microinjection of V-ATPase B and V-ATPase D dsRNA to investigate the effectiveness of RNAi in P. maidis. Real-time quantitative reverse transcriptase-PCR (qRT-PCR) analysis indicated that microinjection of V-ATPase dsRNA led to a minimum reduction of 27-fold in the normalized abundance of V-ATPase transcripts two days post injection, while ingestion of dsRNA resulted in a two-fold reduction after six days of feeding. While both methods of dsRNA delivery resulted in knockdown of target transcripts, the injection method was more rapid and effective. The reduction in V-ATPase transcript abundance resulted in observable phenotypes. Specifically, the development of nymphs injected with 200 ng of either V-ATPase B or D dsRNA was impaired, resulting in higher mortality and lower fecundity than control insects injected with GFP dsRNA. Microscopic examination of these insects revealed that female reproductive organs did not develop normally. The successful development of RNAi in P. maidis to target specific genes will enable the development of new insect control strategies and functional analysis of vital genes and genes associated with interactions between P. maidis and MMV. | |
| 651 | 4 | |a Kansas | |
| 651 | 4 | |a United States--US | |
| 653 | |a Fecundity | ||
| 653 | |a Double-stranded RNA | ||
| 653 | |a Geographical distribution | ||
| 653 | |a Open reading frames | ||
| 653 | |a RNA-mediated interference | ||
| 653 | |a Transcription | ||
| 653 | |a Population density | ||
| 653 | |a Sorghum | ||
| 653 | |a Genomes | ||
| 653 | |a Adenosine triphosphatase | ||
| 653 | |a H+-transporting ATPase | ||
| 653 | |a Gene expression | ||
| 653 | |a Microinjection | ||
| 653 | |a Hemiptera | ||
| 653 | |a Reproductive organs | ||
| 653 | |a Insects | ||
| 653 | |a Plant pathology | ||
| 653 | |a Enzymes | ||
| 653 | |a Infections | ||
| 653 | |a Drosophila melanogaster | ||
| 653 | |a Insecticides | ||
| 653 | |a Protons | ||
| 653 | |a Corn | ||
| 653 | |a Injection | ||
| 653 | |a Nilaparvata lugens | ||
| 653 | |a Delphacidae | ||
| 653 | |a Fulgoroidea | ||
| 653 | |a RNA-directed DNA polymerase | ||
| 653 | |a Phenotypes | ||
| 653 | |a Insect control | ||
| 653 | |a Ingestion | ||
| 653 | |a Genes | ||
| 653 | |a Effectiveness | ||
| 653 | |a Functional analysis | ||
| 653 | |a Peregrinus maidis | ||
| 653 | |a Zea mays | ||
| 653 | |a Environmental | ||
| 700 | 1 | |a Rotenberg, Dorith | |
| 700 | 1 | |a Afsharifar, Alireza | |
| 700 | 1 | |a Barandoc-Alviar, Karen | |
| 700 | 1 | |a Whitfield, Anna E | |
| 773 | 0 | |t PLoS One |g vol. 8, no. 8 (Aug 2013), p. e70243 | |
| 786 | 0 | |d ProQuest |t Health & Medical Collection | |
| 856 | 4 | 1 | |3 Citation/Abstract |u https://www.proquest.com/docview/1430411749/abstract/embedded/75I98GEZK8WCJMPQ?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text |u https://www.proquest.com/docview/1430411749/fulltext/embedded/75I98GEZK8WCJMPQ?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text - PDF |u https://www.proquest.com/docview/1430411749/fulltextPDF/embedded/75I98GEZK8WCJMPQ?source=fedsrch |