Antibacterial Activity of Alkaloid Fractions from Berberis microphylla G. Forst and Study of Synergism with Ampicillin and Cephalothin

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Publicado en:Molecules vol. 21, no. 1 (2016), p. 76
Autor principal: Manosalva, Loreto
Otros Autores: Mutis, Ana, Urzúa, Alejandro, Fajardo, Victor, Quiroz, Andres
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MDPI AG
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Acceso en línea:Citation/Abstract
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100 1 |a Manosalva, Loreto 
245 1 |a Antibacterial Activity of Alkaloid Fractions from Berberis microphylla G. Forst and Study of Synergism with Ampicillin and Cephalothin 
260 |b MDPI AG  |c 2016 
513 |a Journal Article 
520 3 |a Berberis microphylla is a native plant that grows in Patagonia and is commonly used by aboriginal ethnic groups in traditional medicine as an antiseptic for different diseases. The present study evaluated the antibacterial and synergistic activity of alkaloid extracts of B. microphylla leaves, stems and roots used either individually or in combination with antibiotics against Gram-positive and Gram-negative bacteria. The in vitro antibacterial activities of leaf, stem and root alkaloid extracts had significant activity only against Gram-positive bacteria. Disc diffusion tests demonstrated that the root extract showed similar activity against B. cereus and S. epidermidis compared to commercial antibiotics, namely ampicillin and cephalothin, and pure berberine, the principal component of the alkaloid extracts, was found to be active only against S. aureus and S. epidermidis with similar activity to that of the root extract. The minimum inhibitory concentrations (MICs) of the alkaloid extracts ranged from 333 to 83 μg/mL, whereas minimum bactericidal concentrations (MBCs) varied from 717 to 167 μg/mL. In addition, synergistic or indifferent effects between the alkaloid extracts and antibiotics against bacterial strains were confirmed. 
651 4 |a Chile 
653 |a Gram-positive bacteria 
653 |a Medicine 
653 |a Gram-negative bacteria 
653 |a Antibiotics 
653 |a Bacteria 
653 |a Leaves 
700 1 |a Mutis, Ana 
700 1 |a Urzúa, Alejandro 
700 1 |a Fajardo, Victor 
700 1 |a Quiroz, Andres 
773 0 |t Molecules  |g vol. 21, no. 1 (2016), p. 76 
786 0 |d ProQuest  |t Health & Medical Collection 
856 4 1 |3 Citation/Abstract  |u https://www.proquest.com/docview/1764371663/abstract/embedded/WAQKWGCDE3OCLOOD?source=fedsrch 
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