GlycCompSoft: Software for Automated Comparison of Low Molecular Weight Heparins Using Top-Down LC/MS Data

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Publicat a:PLoS One vol. 11, no. 12 (Dec 2016), p. e0167727
Autor principal: Wang, Xiaohua
Altres autors: Liu, Xinyue, Li, Lingyun, Zhang, Fuming, Hu, Min, Ren, Fuji, Chi, Lianli, Linhardt, Robert J
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Public Library of Science
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024 7 |a 10.1371/journal.pone.0167727  |2 doi 
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045 2 |b d20161201  |b d20161231 
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100 1 |a Wang, Xiaohua 
245 1 |a GlycCompSoft: Software for Automated Comparison of Low Molecular Weight Heparins Using Top-Down LC/MS Data 
260 |b Public Library of Science  |c Dec 2016 
513 |a Journal Article 
520 3 |a Low molecular weight heparins are complex polycomponent drugs that have recently become amenable to top-down analysis using liquid chromatography-mass spectrometry. Even using open source deconvolution software, DeconTools, and automatic structural assignment software, GlycReSoft, the comparison of two or more low molecular weight heparins is extremely time-consuming, taking about a week for an expert analyst and provides no guarantee of accuracy. Efficient data processing tools are required to improve analysis. This study uses the programming language of Microsoft Excel™ Visual Basic for Applications to extend its standard functionality for macro functions and specific mathematical modules for mass spectrometric data processing. The program developed enables the comparison of top-down analytical glycomics data on two or more low molecular weight heparins. The current study describes a new program, GlycCompSoft, which has a low error rate with good time efficiency in the automatic processing of large data sets. The experimental results based on three lots of Lovenox®, Clexane® and three generic enoxaparin samples show that the run time of GlycCompSoft decreases from 11 to 2 seconds when the data processed decreases from 18000 to 1500 rows. 
610 4 |a Boston University Rensselaer Polytechnic Institute Food & Drug Administration--FDA 
651 4 |a United States--US 
651 4 |a New York 
651 4 |a China 
653 |a Mass spectrometry 
653 |a Software packages 
653 |a Liquid chromatography 
653 |a Data processing 
653 |a Application programming interface 
653 |a Bioinformatics 
653 |a Functions (mathematics) 
653 |a Low molecular weights 
653 |a Ions 
653 |a Open source software 
653 |a Software 
653 |a Interdisciplinary aspects 
653 |a Scientific imaging 
653 |a Chromatography 
653 |a Packaging 
653 |a Visual Basic for Applications 
653 |a Computer programs 
653 |a Fourier transforms 
653 |a Analytical chemistry 
653 |a Hypotheses 
653 |a Automation 
653 |a Programming languages 
653 |a Mass spectroscopy 
653 |a Economic 
653 |a Molecular weight 
653 |a Visual programming languages 
653 |a Biotechnology 
653 |a Proteomics 
653 |a Heparin 
653 |a Anticoagulants 
653 |a Laboratories 
700 1 |a Liu, Xinyue 
700 1 |a Li, Lingyun 
700 1 |a Zhang, Fuming 
700 1 |a Hu, Min 
700 1 |a Ren, Fuji 
700 1 |a Chi, Lianli 
700 1 |a Linhardt, Robert J 
773 0 |t PLoS One  |g vol. 11, no. 12 (Dec 2016), p. e0167727 
786 0 |d ProQuest  |t Health & Medical Collection 
856 4 1 |3 Citation/Abstract  |u https://www.proquest.com/docview/1848103727/abstract/embedded/6A8EOT78XXH2IG52?source=fedsrch 
856 4 0 |3 Full Text  |u https://www.proquest.com/docview/1848103727/fulltext/embedded/6A8EOT78XXH2IG52?source=fedsrch 
856 4 0 |3 Full Text - PDF  |u https://www.proquest.com/docview/1848103727/fulltextPDF/embedded/6A8EOT78XXH2IG52?source=fedsrch