miR-378a enhances the sensitivity of liver cancer to sorafenib by targeting VEGFR, PDGFRβ and c-Raf
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| Publicado en: | Molecular Medicine Reports vol. 17, no. 3 (2018), p. 4581 |
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| Autor principal: | |
| Otros Autores: | , , , , |
| Publicado: |
Spandidos Publications UK Ltd.
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| Materias: | |
| Acceso en línea: | Citation/Abstract Full Text Full Text - PDF |
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| LEADER | 00000nab a2200000uu 4500 | ||
|---|---|---|---|
| 001 | 1994736655 | ||
| 003 | UK-CbPIL | ||
| 022 | |a 1791-2997 | ||
| 022 | |a 1791-3004 | ||
| 024 | 7 | |a 10.3892/mmr.2018.8390 |2 doi | |
| 035 | |a 1994736655 | ||
| 045 | 2 | |b d20180101 |b d20181231 | |
| 100 | 1 | |a Fu, Hongxia | |
| 245 | 1 | |a miR-378a enhances the sensitivity of liver cancer to sorafenib by targeting VEGFR, PDGFRβ and c-Raf | |
| 260 | |b Spandidos Publications UK Ltd. |c 2018 | ||
| 513 | |a Journal Article | ||
| 520 | 3 | |a Liver cancer is a globally prevalent cancer with poor prognosis. The present study investigated the link between microRNA-378a (miR-378a) expression and the sensitivity of hepatocellular carcinoma (HCC) and hepatoblastoma (HB) cancers to sorafenib therapy. miR-378a expression was determined in liver tissue samples from healthy candidates and patients with liver cancer using the reverse transcription-quantitative polymerase chain reaction. The antitumor effects of miR-378a alone and in combination with sorafenib were investigated in the HB cell line HepG2 and the HCC cell line SMMC-7721 with methyl thiazoyl tetrazolium, colony formation, flow cytometry and Transwell migration assays. The underlying mechanisms were investigated using western blot analysis. miR-378a expression was decreased in tissue samples from patients with liver cancer. HCC and HB cell line proliferation and invasion ability was inhibited by miR-378a. The combination of miR-378a and sorafenib provided the greatest inhibition. Western blot indicated that mitogen activated protein kinase signaling pathway proteins, vascular endothelial growth factor receptor, platelet derived growth factor receptor β, Raf-1 proto-oncogene, serine/threonine kinase and matrix metallopeptidase 2 were regulated by miR-378a alone and to a greater extent when combined with sorafenib. Results suggest that miR-378a can inhibit liver cancer cell growth and enhance the sensitivity of liver cancer cells to sorafenib-based chemotherapies. | |
| 651 | 4 | |a United States--US | |
| 651 | 4 | |a China | |
| 653 | |a Standard deviation | ||
| 653 | |a Vascular endothelial growth factor | ||
| 653 | |a Protein-serine/threonine kinase | ||
| 653 | |a Liver cancer | ||
| 653 | |a Antitumor activity | ||
| 653 | |a MicroRNAs | ||
| 653 | |a Polymerase chain reaction | ||
| 653 | |a Raf protein | ||
| 653 | |a Flow cytometry | ||
| 653 | |a Apoptosis | ||
| 653 | |a Kinases | ||
| 653 | |a Proteins | ||
| 653 | |a Growth factors | ||
| 653 | |a Medical prognosis | ||
| 653 | |a Hepatocellular carcinoma | ||
| 653 | |a Studies | ||
| 653 | |a Vascular endothelial growth factor receptors | ||
| 653 | |a Metalloproteinase | ||
| 653 | |a Hepatocytes | ||
| 653 | |a Tumors | ||
| 653 | |a Signal transduction | ||
| 653 | |a Reverse transcription | ||
| 653 | |a miRNA | ||
| 653 | |a Targeted cancer therapy | ||
| 653 | |a Inhibitor drugs | ||
| 700 | 1 | |a Zhang, Jicai | |
| 700 | 1 | |a Pan, Tongshan | |
| 700 | 1 | |a Ai, Shuying | |
| 700 | 1 | |a Tang, Li | |
| 700 | 1 | |a Wang, Fengqin | |
| 773 | 0 | |t Molecular Medicine Reports |g vol. 17, no. 3 (2018), p. 4581 | |
| 786 | 0 | |d ProQuest |t Health & Medical Collection | |
| 856 | 4 | 1 | |3 Citation/Abstract |u https://www.proquest.com/docview/1994736655/abstract/embedded/75I98GEZK8WCJMPQ?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text |u https://www.proquest.com/docview/1994736655/fulltext/embedded/75I98GEZK8WCJMPQ?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text - PDF |u https://www.proquest.com/docview/1994736655/fulltextPDF/embedded/75I98GEZK8WCJMPQ?source=fedsrch |