Computer simulation of synchronization of Na/K pump molecules

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Publicado en:Journal of Bioenergetics and Biomembranes vol. 40, no. 4 (Aug 2008), p. 337
Autor principal: Chen, Wei
Otros Autores: Huang, Feiran
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Springer Nature B.V.
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Acceso en línea:Citation/Abstract
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100 1 |a Chen, Wei 
245 1 |a Computer simulation of synchronization of Na/K pump molecules 
260 |b Springer Nature B.V.  |c Aug 2008 
513 |a Journal Article 
520 3 |a The behavior of Na/K pump currents when exposed to an oscillating electric field is studied by computer simulation. The pump current from a single pump molecule was sketched based on previous experimental results. The oscillating electric field is designed as a symmetric, dichotomous waveform varying the membrane potential from -30 to -150 mV around the membrane resting potential of -90 mV. Based on experimental results from skeletal muscle fibers, the energy needed to overcome the electrochemical potentials for the Na and K-transports are calculated in response to the field's two half-cycles. We found that a specially designed oscillating electric field can eventually synchronize the pump molecules so that all the individual pumps run at the same pumping rate and phase as the field oscillation. They extrude Na ions during the positive half-cycle and pump in K ions during the negative half-cycle. The field can force the two ion-transports into the corresponding half-cycles, respectively, but cannot determine their detailed positions. In other words, the oscillating electric field can synchronize pumps in terms of their pumping loops but not at a specific step in the loop. These results are consistent with our experimental results in measurement of the pump currents. (PUBLICATION ABSTRACT)   The behavior of Na/K pump currents when exposed to an oscillating electric field is studied by computer simulation. The pump current from a single pump molecule was sketched based on previous experimental results. The oscillating electric field is designed as a symmetric, dichotomous waveform varying the membrane potential from -30 to -150 mV around the membrane resting potential of -90 mV. Based on experimental results from skeletal muscle fibers, the energy needed to overcome the electrochemical potentials for the Na and K-transports are calculated in response to the field's two half-cycles. We found that a specially designed oscillating electric field can eventually synchronize the pump molecules so that all the individual pumps run at the same pumping rate and phase as the field oscillation. They extrude Na ions during the positive half-cycle and pump in K ions during the negative half-cycle. The field can force the two ion-transports into the corresponding half-cycles, respectively, but cannot determine their detailed positions. In other words, the oscillating electric field can synchronize pumps in terms of their pumping loops but not at a specific step in the loop. These results are consistent with our experimental results in measurement of the pump currents. 
650 1 2 |a Biological Clocks  |x physiology 
650 2 2 |a Computer Simulation 
650 1 2 |a Ion Channel Gating  |x physiology 
650 1 2 |a Membrane Potentials  |x physiology 
650 1 2 |a Models, Biological 
650 2 2 |a Models, Chemical 
650 2 2 |a Potassium  |x chemistry 
650 1 2 |a Potassium  |x metabolism 
650 2 2 |a Sodium  |x chemistry 
650 1 2 |a Sodium  |x metabolism 
650 2 2 |a Sodium-Potassium-Exchanging ATPase  |x chemistry 
650 1 2 |a Sodium-Potassium-Exchanging ATPase  |x physiology 
653 |a Membranes 
653 |a Electric fields 
653 |a Studies 
653 |a Simulation 
653 |a Molecules 
653 |a Electrochemistry 
653 |a Pumps 
653 |a Ions 
653 |a Environmental 
700 1 |a Huang, Feiran 
773 0 |t Journal of Bioenergetics and Biomembranes  |g vol. 40, no. 4 (Aug 2008), p. 337 
786 0 |d ProQuest  |t Science Database 
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