Deciphering the scopolamine challenge rat model by preclinical functional MRI
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| Publicado en: | Scientific Reports (Nature Publisher Group) vol. 11, no. 1 (2021) |
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| Otros Autores: | , , , , |
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| Acceso en línea: | Citation/Abstract Full Text - PDF |
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| 001 | 2531842056 | ||
| 003 | UK-CbPIL | ||
| 022 | |a 2045-2322 | ||
| 024 | 7 | |a 10.1038/s41598-021-90273-9 |2 doi | |
| 035 | |a 2531842056 | ||
| 045 | 2 | |b d20210101 |b d20211231 | |
| 084 | |a 274855 |2 nlm | ||
| 100 | 1 | |a Somogyi Gergely |u Gedeon Richter Plc., Pharmacological and Drug Safety Research, Budapest 10, Hungary (GRID:grid.418137.8) (ISNI:0000 0004 0621 5862) | |
| 245 | 1 | |a Deciphering the scopolamine challenge rat model by preclinical functional MRI | |
| 260 | |b Nature Publishing Group |c 2021 | ||
| 513 | |a Journal Article | ||
| 520 | 3 | |a During preclinical drug testing, the systemic administration of scopolamine (SCO), a cholinergic antagonist, is widely used. However, it suffers important limitations, like non-specific behavioural effects partly due to its peripheral side-effects. Therefore, neuroimaging measures would enhance its translational value. To this end, in Wistar rats, we measured whisker-stimulation induced functional MRI activation after SCO, peripherally acting butylscopolamine (BSCO), or saline administration in a cross-over design. Besides the commonly used gradient-echo echo-planar imaging (GE EPI), we also used an arterial spin labeling method in isoflurane anesthesia. With the GE EPI measurement, SCO decreased the evoked BOLD response in the barrel cortex (BC), while BSCO increased it in the anterior cingulate cortex. In a second experiment, we used GE EPI and spin-echo (SE) EPI sequences in a combined (isoflurane + i.p. dexmedetomidine) anesthesia to account for anesthesia-effects. Here, we also examined the effect of donepezil. In the combined anesthesia, with the GE EPI, SCO decreased the activation in the BC and the inferior colliculus (IC). BSCO reduced the response merely in the IC. Our results revealed that SCO attenuated the evoked BOLD activation in the BC as a probable central effect in both experiments. The likely peripheral vascular actions of SCO with the given fMRI sequences depended on the type of anesthesia or its dose. | |
| 653 | |a Inferior colliculus | ||
| 653 | |a Cortex (barrel) | ||
| 653 | |a Functional magnetic resonance imaging | ||
| 653 | |a Anesthesia | ||
| 653 | |a Isoflurane | ||
| 653 | |a Animal models | ||
| 653 | |a Scopolamine | ||
| 653 | |a Neuroimaging | ||
| 653 | |a Cortex (cingulate) | ||
| 653 | |a Donepezil | ||
| 653 | |a Animals | ||
| 653 | |a Experiments | ||
| 653 | |a Drug testing | ||
| 653 | |a Drug dosages | ||
| 653 | |a Somatosensory cortex | ||
| 653 | |a Side effects | ||
| 653 | |a Spin labeling | ||
| 653 | |a Environmental | ||
| 700 | 1 | |a Hlatky Dávid |u Gedeon Richter Plc., Pharmacological and Drug Safety Research, Budapest 10, Hungary (GRID:grid.418137.8) (ISNI:0000 0004 0621 5862) | |
| 700 | 1 | |a Spisák Tamás |u Gedeon Richter Plc., Pharmacological and Drug Safety Research, Budapest 10, Hungary (GRID:grid.418137.8) (ISNI:0000 0004 0621 5862); University Hospital Essen, Department of Diagnostic and Interventional Radiology and Neuroradiology, Essen, Germany (GRID:grid.410718.b) (ISNI:0000 0001 0262 7331) | |
| 700 | 1 | |a Spisák Zsófia |u Gedeon Richter Plc., Pharmacological and Drug Safety Research, Budapest 10, Hungary (GRID:grid.418137.8) (ISNI:0000 0004 0621 5862) | |
| 700 | 1 | |a Nyitrai Gabriella |u Gedeon Richter Plc., Pharmacological and Drug Safety Research, Budapest 10, Hungary (GRID:grid.418137.8) (ISNI:0000 0004 0621 5862) | |
| 700 | 1 | |a Czurkó András |u Gedeon Richter Plc., Pharmacological and Drug Safety Research, Budapest 10, Hungary (GRID:grid.418137.8) (ISNI:0000 0004 0621 5862) | |
| 773 | 0 | |t Scientific Reports (Nature Publisher Group) |g vol. 11, no. 1 (2021) | |
| 786 | 0 | |d ProQuest |t Science Database | |
| 856 | 4 | 1 | |3 Citation/Abstract |u https://www.proquest.com/docview/2531842056/abstract/embedded/75I98GEZK8WCJMPQ?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text - PDF |u https://www.proquest.com/docview/2531842056/fulltextPDF/embedded/75I98GEZK8WCJMPQ?source=fedsrch |