Derepressed 2-deoxyglucose-resistant mutants of Aspergillus niger with altered hexokinase and acid phosphatase activity in hyperproduction of β-fructofuranosidase
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| Udgivet i: | Applied Biochemistry and Biotechnology vol. 118, no. 1-3 (Jul 2004), p. 89 |
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Springer Nature B.V.
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| Online adgang: | Citation/Abstract Full Text - PDF |
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| 100 | 1 | |a Ashokkumar, B |u Madurai Kamaraj University, Department of Microbial Technology, School of Biological Sciences, Centre for Excellence in Genomic Sciences, Madurai, (GRID:grid.10214.36) (ISNI:0000000121867912) | |
| 245 | 1 | |a Derepressed 2-deoxyglucose-resistant mutants of <i>Aspergillus niger</i> with altered hexokinase and acid phosphatase activity in hyperproduction of β-fructofuranosidase | |
| 260 | |b Springer Nature B.V. |c Jul 2004 | ||
| 513 | |a Journal Article | ||
| 520 | 3 | |a Aspergillus niger NRRL330 produces extracellular β-fructofuranosidase (Ffase), and its production is subject to repression by hexoses in the medium. After ultraviolet mutagenization and selection, seven derepressed mutants resistant to 2-deoxyglucose (2-DG) were isolated on Czapek’s minimal medium containing glycerol. One of the mutants, designated DGRA-1, produced higher levels of Ffase. A considerable difference occurred in the mutants with reference to hexokinase and intracellular acid phosphatase activities. The hexokinase activity of the mutant DGRA-1 (0.69 U/mg) was 1.8-fold higher than the wild type (0.38U/mg). Intracellular acid phosphatase activity of the mutant DGRA-1 (0.83 U/g of mycelia) was twofold higher than that of the wild type (0.42U/g of mycelia), suggesting that phosphorylation and dephosphorylation steps could attribute to the 2-DG resistance of A. niger. However, additional mutations could account for the increased production of Ffase in the mutant DGRA-1. | |
| 653 | |a Hexokinase | ||
| 653 | |a Phosphorylation | ||
| 653 | |a b-Fructofuranosidase | ||
| 653 | |a Phosphatase | ||
| 653 | |a Acid phosphatase | ||
| 653 | |a Glycerol | ||
| 653 | |a Dephosphorylation | ||
| 653 | |a Invertase | ||
| 653 | |a Resistant mutant | ||
| 653 | |a Deoxyglucose | ||
| 653 | |a Mutation | ||
| 653 | |a Kinases | ||
| 653 | |a Intracellular | ||
| 653 | |a Hexoses | ||
| 653 | |a Aspergillus niger | ||
| 653 | |a Environmental | ||
| 700 | 1 | |a Senthilkumar, S R |u Madurai Kamaraj University, Department of Microbial Technology, School of Biological Sciences, Centre for Excellence in Genomic Sciences, Madurai, (GRID:grid.10214.36) (ISNI:0000000121867912) | |
| 700 | 1 | |a Gunasekaran, P |u Madurai Kamaraj University, Department of Microbial Technology, School of Biological Sciences, Centre for Excellence in Genomic Sciences, Madurai, (GRID:grid.10214.36) (ISNI:0000000121867912) | |
| 773 | 0 | |t Applied Biochemistry and Biotechnology |g vol. 118, no. 1-3 (Jul 2004), p. 89 | |
| 786 | 0 | |d ProQuest |t Science Database | |
| 856 | 4 | 1 | |3 Citation/Abstract |u https://www.proquest.com/docview/2664708293/abstract/embedded/75I98GEZK8WCJMPQ?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text - PDF |u https://www.proquest.com/docview/2664708293/fulltextPDF/embedded/75I98GEZK8WCJMPQ?source=fedsrch |