Novel Insights into the Antimicrobial and Antibiofilm Activity of Pyrroloquinoline Quinone (PQQ); In Vitro, In Silico, and Shotgun Proteomic Studies
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| Publicado en: | Biomolecules vol. 14, no. 8 (2024), p. 1018 |
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| Autor principal: | |
| Otros Autores: | , , , , , , , , |
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MDPI AG
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| Acceso en línea: | Citation/Abstract Full Text + Graphics Full Text - PDF |
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| 001 | 3097839867 | ||
| 003 | UK-CbPIL | ||
| 022 | |a 2218-273X | ||
| 024 | 7 | |a 10.3390/biom14081018 |2 doi | |
| 035 | |a 3097839867 | ||
| 045 | 2 | |b d20240101 |b d20241231 | |
| 084 | |a 231434 |2 nlm | ||
| 100 | 1 | |a Labib, Mai M |u Department of Bioinformatics, Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Centre (ARC), Cairo 12619, Egypt; <email>mailabib@ageri.sci.eg</email> | |
| 245 | 1 | |a Novel Insights into the Antimicrobial and Antibiofilm Activity of Pyrroloquinoline Quinone (PQQ); <i>In Vitro</i>, <i>In Silico</i>, and Shotgun Proteomic Studies | |
| 260 | |b MDPI AG |c 2024 | ||
| 513 | |a Journal Article | ||
| 520 | 3 | |a Microbial infections pose a significant global health threat, affecting millions of individuals and leading to substantial mortality rates. The increasing resistance of microorganisms to conventional treatments requires the development of novel antimicrobial agents. Pyrroloquinoline quinone (PQQ), a natural medicinal drug involved in various cellular processes, holds promise as a potential antimicrobial agent. In the present study, our aim was, for the first time, to explore the antimicrobial activity of PQQ against 29 pathogenic microbes, including 13 fungal strains, 8 Gram-positive bacteria, and 8 Gram-negative bacteria. Our findings revealed potent antifungal properties of PQQ, particularly against Syncephalastrum racemosum, Talaromyces marneffei, Candida lipolytica, and Trichophyton rubrum. The MIC values varied between fungal strains, and T. marneffei exhibited a lower MIC, indicating a greater susceptibility to PQQ. In addition, PQQ exhibited notable antibacterial activity against Gram-positive and -negative bacteria, with a prominent inhibition observed against Staphylococcus epidermidis, Proteus vulgaris, and MRSA strains. Remarkably, PQQ demonstrated considerable biofilm inhibition against the MRSA, S. epidermidis, and P. vulgaris strains. Transmission electron microscopy (TEM) studies revealed that PQQ caused structural damage and disrupted cell metabolism in bacterial cells, leading to aberrant morphology, compromised cell membrane integrity, and leakage of cytoplasmic contents. These findings were further affirmed by shotgun proteomic analysis, which revealed that PQQ targets several important cellular processes in bacteria, including membrane proteins, ATP metabolic processes, DNA repair processes, metal-binding proteins, and stress response. Finally, detailed molecular modeling investigations indicated that PQQ exhibits a substantial binding affinity score for key microbial targets, including the mannoprotein Mp1P, the transcriptional regulator TcaR, and the endonuclease PvuRTs1I. Taken together, our study underscores the effectiveness of PQQ as a broad-spectrum antimicrobial agent capable of combating pathogenic fungi and bacteria, while also inhibiting biofilm formation and targeting several critical biological processes, making it a promising therapeutic option for biofilm-related infections. | |
| 653 | |a Fungi | ||
| 653 | |a Public health | ||
| 653 | |a Antibacterial activity | ||
| 653 | |a Drug resistance | ||
| 653 | |a Bacteria | ||
| 653 | |a Staphylococcus infections | ||
| 653 | |a Gram-positive bacteria | ||
| 653 | |a Gram-negative bacteria | ||
| 653 | |a Molecular modelling | ||
| 653 | |a Fungal infections | ||
| 653 | |a E coli | ||
| 653 | |a Metabolism | ||
| 653 | |a Research & development--R&D | ||
| 653 | |a Cellular stress response | ||
| 653 | |a Performance evaluation | ||
| 653 | |a Proteomics | ||
| 653 | |a COVID-19 | ||
| 653 | |a Cellular biology | ||
| 653 | |a Endonuclease | ||
| 653 | |a Pyrroloquinoline quinone | ||
| 653 | |a DNA repair | ||
| 653 | |a Antibiotics | ||
| 653 | |a Minimum inhibitory concentration | ||
| 653 | |a Bacterial infections | ||
| 653 | |a Medical research | ||
| 653 | |a Flexibility | ||
| 653 | |a Biofilms | ||
| 653 | |a Antimicrobial agents | ||
| 653 | |a Transmission electron microscopy | ||
| 653 | |a Membrane proteins | ||
| 653 | |a Antifungal activity | ||
| 653 | |a Streptococcus infections | ||
| 653 | |a Microorganisms | ||
| 653 | |a Strains (organisms) | ||
| 653 | |a Antimicrobial activity | ||
| 653 | |a Tropical diseases | ||
| 653 | |a Disease transmission | ||
| 653 | |a Cell membranes | ||
| 653 | |a Antifungal agents | ||
| 653 | |a Staphylococcus epidermidis | ||
| 700 | 1 | |a Alqahtani, Alaa M |u Department of Pharmaceutical Sciences, Faculty of Pharmacy, Umm Al-Qura University, Makkah 21955, Saudi Arabia; <email>amqahtani@uqu.edu.sa</email> | |
| 700 | 1 | |a Abo Nahas, Hebatallah H |u Zoology Department, Faculty of Science, Port Said University, Port Said 42526, Egypt; <email>heba.hassan@sci.psu.edu.eg</email> | |
| 700 | 1 | |a Aldossari, Rana M |u Department of Pharmacology and Toxicology, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj 11942, Saudi Arabia; <email>r.alsaffar@psau.edu.sa</email> | |
| 700 | 1 | |a Bandar Fahad Almiman |u Biology Department, College of Science, Al-Baha University, Al Bahah 65779, Saudi Arabia; <email>balmiman@bu.edu.sa</email> | |
| 700 | 1 | |a Sarah Ayman Alnumaani |u Department of Medical Microbiology, Faculty of Medicine, University of Jeddah, Jeddah 23218, Saudi Arabia; <email>anomani@uj.edu.sa</email> | |
| 700 | 1 | |a El-Nablaway, Mohammad |u Department of Basic Medical Sciences, College of Medicine, AlMaarefa University, P.O. Box 71666, Riyadh 11597, Saudi Arabia; <email>mnablawi@um.edu.sa</email>; Department of Medical Biochemistry, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt | |
| 700 | 1 | |a Al-Olayan, Ebtesam |u Department of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia; <email>eolayan@ksu.edu.sa</email> | |
| 700 | 1 | |a Alsunbul, Maha |u Department of Pharmaceutical Sciences, College of Pharmacy, Princess Nourah Bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia; <email>maalsonbel@pnu.edu.sa</email> | |
| 700 | 1 | |a Saied, Essa M |u Chemistry Department, Faculty of Science, Suez Canal University, Ismailia 41522, Egypt; Institute for Chemistry, Humboldt Universität zu Berlin, 12489 Berlin, Germany | |
| 773 | 0 | |t Biomolecules |g vol. 14, no. 8 (2024), p. 1018 | |
| 786 | 0 | |d ProQuest |t Health & Medical Collection | |
| 856 | 4 | 1 | |3 Citation/Abstract |u https://www.proquest.com/docview/3097839867/abstract/embedded/L8HZQI7Z43R0LA5T?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text + Graphics |u https://www.proquest.com/docview/3097839867/fulltextwithgraphics/embedded/L8HZQI7Z43R0LA5T?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text - PDF |u https://www.proquest.com/docview/3097839867/fulltextPDF/embedded/L8HZQI7Z43R0LA5T?source=fedsrch |