Targeting Hypoglycemic Natural Products from the Cloud Forest Plants Using Chemotaxonomic Computer-Assisted Selection
Guardado en:
| Publicado en: | International Journal of Molecular Sciences vol. 25, no. 20 (2024), p. 10881 |
|---|---|
| Autor principal: | |
| Otros Autores: | , , , , , |
| Publicado: |
MDPI AG
|
| Materias: | |
| Acceso en línea: | Citation/Abstract Full Text + Graphics Full Text - PDF |
| Etiquetas: |
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
MARC
| LEADER | 00000nab a2200000uu 4500 | ||
|---|---|---|---|
| 001 | 3120650260 | ||
| 003 | UK-CbPIL | ||
| 022 | |a 1661-6596 | ||
| 022 | |a 1422-0067 | ||
| 024 | 7 | |a 10.3390/ijms252010881 |2 doi | |
| 035 | |a 3120650260 | ||
| 045 | 2 | |b d20241015 |b d20241031 | |
| 084 | |a 231645 |2 nlm | ||
| 100 | 1 | |a Mayo-Montor, Cecilia I |u Red de Estudios Moleculares Avanzados, Instituto de Ecología A.C., Xalapa 91073, Mexico; <email>cecilia.mayo@posgrado.ecologia.edu.mx</email> (C.I.M.-M.); <email>abraham.vidal@inecol.mx</email> (A.V.-L.); <email>jose.barreda@posgrado.ecologia.edu.mx</email> (J.M.B.-C.) | |
| 245 | 1 | |a Targeting Hypoglycemic Natural Products from the Cloud Forest Plants Using Chemotaxonomic Computer-Assisted Selection | |
| 260 | |b MDPI AG |c 2024 | ||
| 513 | |a Journal Article | ||
| 520 | 3 | |a The cloud forest (CF), a hugely biodiverse ecosystem, is a hotspot of unexplored plants with potential for discovering pharmacologically active compounds. Without sufficient ethnopharmacological information, developing strategies for rationally selecting plants for experimental studies is crucial. With this goal, a CF metabolites library was created, and a ligand-based virtual screening was conducted to identify molecules with potential hypoglycemic activity. From the most promising botanical families, plants were collected, methanolic extracts were prepared, and hypoglycemic activity was evaluated through in vitro enzyme inhibition assays on α-amylase, α-glucosidase, and dipeptidyl peptidase IV (DPP-IV). Metabolomic analyses were performed to identify the dominant metabolites in the species with the best inhibitory activity profile, and their affinity for the molecular targets was evaluated using ensemble molecular docking. This strategy led to the identification of twelve plants (in four botanical families) with hypoglycemic activity. Sida rhombifolia (Malvaceae) stood out for its DPP-IV selective inhibition versus S. glabra. A comparison of chemical profiles led to the annotation of twenty-seven metabolites over-accumulated in S. rhombifolia compared to S. glabra, among which acanthoside D and cis-tiliroside were noteworthy for their potential selective inhibition due to their specific intermolecular interactions with relevant amino acids of DPP-IV. The workflow used in this study presents a novel targeting strategy for identifying novel bioactive natural sources, which can complement the conventional selection criteria used in Natural Product Chemistry. | |
| 651 | 4 | |a Mexico | |
| 653 | |a Glucagon | ||
| 653 | |a Hyperglycemia | ||
| 653 | |a Metabolism | ||
| 653 | |a Flowers & plants | ||
| 653 | |a Libraries | ||
| 653 | |a Natural products | ||
| 653 | |a Enzymes | ||
| 653 | |a Metabolites | ||
| 653 | |a Carbohydrates | ||
| 700 | 1 | |a Vidal-Limon, Abraham |u Red de Estudios Moleculares Avanzados, Instituto de Ecología A.C., Xalapa 91073, Mexico; <email>cecilia.mayo@posgrado.ecologia.edu.mx</email> (C.I.M.-M.); <email>abraham.vidal@inecol.mx</email> (A.V.-L.); <email>jose.barreda@posgrado.ecologia.edu.mx</email> (J.M.B.-C.) | |
| 700 | 1 | |a Loyola-Vargas, Víctor Manuel |u Unidad de Biología Integrativa, Centro de Investigación Científica de Yucatán, Mérida 97205, Mexico; <email>vmloyola@cicy.mx</email> | |
| 700 | 1 | |a Carmona-Hernández, Oscar |u Facultad de Biología, Universidad Veracruzana, Xalapa 91090, Mexico; <email>ocarmona@uv.mx</email> | |
| 700 | 1 | |a José Martín Barreda-Castillo |u Red de Estudios Moleculares Avanzados, Instituto de Ecología A.C., Xalapa 91073, Mexico; <email>cecilia.mayo@posgrado.ecologia.edu.mx</email> (C.I.M.-M.); <email>abraham.vidal@inecol.mx</email> (A.V.-L.); <email>jose.barreda@posgrado.ecologia.edu.mx</email> (J.M.B.-C.) | |
| 700 | 1 | |a Monribot-Villanueva, Juan L |u Red de Estudios Moleculares Avanzados, Instituto de Ecología A.C., Xalapa 91073, Mexico; <email>cecilia.mayo@posgrado.ecologia.edu.mx</email> (C.I.M.-M.); <email>abraham.vidal@inecol.mx</email> (A.V.-L.); <email>jose.barreda@posgrado.ecologia.edu.mx</email> (J.M.B.-C.) | |
| 700 | 1 | |a Guerrero-Analco, José A |u Red de Estudios Moleculares Avanzados, Instituto de Ecología A.C., Xalapa 91073, Mexico; <email>cecilia.mayo@posgrado.ecologia.edu.mx</email> (C.I.M.-M.); <email>abraham.vidal@inecol.mx</email> (A.V.-L.); <email>jose.barreda@posgrado.ecologia.edu.mx</email> (J.M.B.-C.) | |
| 773 | 0 | |t International Journal of Molecular Sciences |g vol. 25, no. 20 (2024), p. 10881 | |
| 786 | 0 | |d ProQuest |t Health & Medical Collection | |
| 856 | 4 | 1 | |3 Citation/Abstract |u https://www.proquest.com/docview/3120650260/abstract/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text + Graphics |u https://www.proquest.com/docview/3120650260/fulltextwithgraphics/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text - PDF |u https://www.proquest.com/docview/3120650260/fulltextPDF/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |