TTF2 drives mitotic replisome disassembly and MiDAS by coupling the TRAIP ubiquitin ligase to Pol epsilon
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| Publicado en: | bioRxiv (Dec 2, 2024) |
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Cold Spring Harbor Laboratory Press
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| Acceso en línea: | Citation/Abstract Full Text - PDF Full text outside of ProQuest |
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|---|---|---|---|
| 001 | 3134981214 | ||
| 003 | UK-CbPIL | ||
| 022 | |a 2692-8205 | ||
| 024 | 7 | |a 10.1101/2024.12.01.626218 |2 doi | |
| 035 | |a 3134981214 | ||
| 045 | 0 | |b d20241202 | |
| 100 | 1 | |a Fujisawa, Ryo | |
| 245 | 1 | |a TTF2 drives mitotic replisome disassembly and MiDAS by coupling the TRAIP ubiquitin ligase to Pol epsilon | |
| 260 | |b Cold Spring Harbor Laboratory Press |c Dec 2, 2024 | ||
| 513 | |a Working Paper | ||
| 520 | 3 | |a Mammalian cells frequently enter mitosis before DNA replication has finished, necessitating the rapid processing of replication forks to facilitate chromosome segregation. The TRAIP ubiquitin ligase induces mitotic replisome disassembly, fork cleavage, and repair via Mitotic DNA Synthesis (MiDAS). Until now, it was unclear how TRAIP is regulated in mitotic cells. Here we show that TRAIP phosphorylation mediates a complex with the TTF2 ATPase and DNA Polymerase epsilon (Pol epsilon). Whereas TTF2 ATPase activity removes RNA polymerase II from mitotic chromosomes, replisome disassembly involves an unanticipated mechanism. The TTF2 amino terminus couples TRAIP to Pol epsilon, via tandem Zinc fingers that recognise phosphorylated TRAIP, and a motif that binds to POLE2. Thereby, TTF2 and Pol epsilon cause TRAIP to ubiquitylate the CDC45-MCM-GINS (CMG) helicase, triggering replisome disassembly and MiDAS. These data identify TTF2 as a multifunctional regulator of chromatin transactions during mitosis.Competing Interest StatementThe authors have declared no competing interest. | |
| 653 | |a RNA polymerase | ||
| 653 | |a DNA biosynthesis | ||
| 653 | |a Phosphorylation | ||
| 653 | |a Chromosomes | ||
| 653 | |a DNA repair | ||
| 653 | |a Mammalian cells | ||
| 653 | |a Zinc finger proteins | ||
| 653 | |a Ubiquitin-protein ligase | ||
| 653 | |a Replication forks | ||
| 653 | |a Chromatin remodeling | ||
| 653 | |a DNA-directed DNA polymerase | ||
| 653 | |a Yeast | ||
| 653 | |a Mitosis | ||
| 653 | |a DNA-directed RNA polymerase | ||
| 653 | |a Cdc45 protein | ||
| 653 | |a DNA helicase | ||
| 653 | |a Cell division | ||
| 700 | 1 | |a Labib, Karim P M | |
| 773 | 0 | |t bioRxiv |g (Dec 2, 2024) | |
| 786 | 0 | |d ProQuest |t Biological Science Database | |
| 856 | 4 | 1 | |3 Citation/Abstract |u https://www.proquest.com/docview/3134981214/abstract/embedded/H09TXR3UUZB2ISDL?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text - PDF |u https://www.proquest.com/docview/3134981214/fulltextPDF/embedded/H09TXR3UUZB2ISDL?source=fedsrch |
| 856 | 4 | 0 | |3 Full text outside of ProQuest |u https://www.biorxiv.org/content/10.1101/2024.12.01.626218v1 |