The Aging Synapse: An Integrated Proteomic And Transcriptomic Analysis

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Publicado no:bioRxiv (Dec 20, 2024)
Autor principal: Caterino, Cinzia
Outros Autores: Ugolini, Martino, Durso, William, Jevdokimenko, Kristina, Groth, Marco, Riege, Konstantin, Goerlach, Matthias, Fornasiero, Eugenio, Ori, Alessandro, Hoffmann, Steve, Cellerino, Alessandro
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Cold Spring Harbor Laboratory Press
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MARC

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022 |a 2692-8205 
024 7 |a 10.1101/2024.12.18.629115  |2 doi 
035 |a 3147576530 
045 0 |b d20241220 
100 1 |a Caterino, Cinzia 
245 1 |a The Aging Synapse: An Integrated Proteomic And Transcriptomic Analysis 
260 |b Cold Spring Harbor Laboratory Press  |c Dec 20, 2024 
513 |a Working Paper 
520 3 |a An important hallmark of aging is the loss of proteostasis, which can lead to the formation of protein aggregates and mitochondrial dysfunction in neurons. Although it is well known that protein synthesis is finely regulated in the brain, especially at synapses, where mRNAs are locally translated in activity-dependent manner, little is known as to the changes in the synaptic proteome and transcriptome during aging. Therefore, this work aims to elucidate the relationship between transcriptome and proteome at soma and synaptic level during aging. Proteomic and transcriptomic data analysis reveal that, in young animals, proteins and transcripts are correlated and synaptic regulation is driven by changes in the soma. During aging, there is a decoupling between transcripts and proteins and between somatic and synaptic compartments. Furthermore, soma-synapse gradient of ribosomal genes changes upon aging, i.e. ribosomal transcripts are less abundant and ribosomal proteins are more abundant in synaptic compartment of old mice with respect to younglings. Additionally, transcriptomics data highlight a difference in the splicing of certain synaptic mRNA with aging. Taken together, our data provide a valuable resource for the study of the aging synapse.Competing Interest StatementThe authors have declared no competing interest. 
653 |a Transcriptomes 
653 |a Proteomes 
653 |a Transcriptomics 
653 |a Ribosomal proteins 
653 |a Protein biosynthesis 
653 |a Aging 
653 |a Synaptogenesis 
653 |a Proteomics 
653 |a Proteins 
700 1 |a Ugolini, Martino 
700 1 |a Durso, William 
700 1 |a Jevdokimenko, Kristina 
700 1 |a Groth, Marco 
700 1 |a Riege, Konstantin 
700 1 |a Goerlach, Matthias 
700 1 |a Fornasiero, Eugenio 
700 1 |a Ori, Alessandro 
700 1 |a Hoffmann, Steve 
700 1 |a Cellerino, Alessandro 
773 0 |t bioRxiv  |g (Dec 20, 2024) 
786 0 |d ProQuest  |t Biological Science Database 
856 4 1 |3 Citation/Abstract  |u https://www.proquest.com/docview/3147576530/abstract/embedded/L8HZQI7Z43R0LA5T?source=fedsrch 
856 4 0 |3 Full text outside of ProQuest  |u https://www.biorxiv.org/content/10.1101/2024.12.18.629115v1