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DANCE: An open-source analysis pipeline and low-cost hardware to quantify aggression and courtship in Drosophila

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Publicado en:bioRxiv (Jan 4, 2025)
Autor principal: R Sai Prathap Yadav
Otros Autores: Dey, Paulami, Ansari, Faizah, Kottat, Tanvi, P Pallavi Prabhu, Vasam, Manohar, Shrinivas Ayyangar, Swathi, Bhaskar S, Prabhu, Krishnananda, Ghosh, Monalisa, Agrawal, Pavan
Publicado:
Cold Spring Harbor Laboratory Press
Materias:
Dopamine receptors
Machine learning
Behavior
RNA-mediated interference
Computational neuroscience
Genetic analysis
Dance
Algorithms
Blister packs
Sensitivity analysis
Insects
Patents
Courtship
Social interactions
Social behavior
Genetic screening
Learning algorithms
Aggression
Drosophila
Acceso en línea:Citation/Abstract
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Resumen:Quantifying animal behaviors is pivotal for identifying the underlying neuronal and genetic mechanisms. Computational approaches have enabled automated analysis of complex behaviors such as aggression and courtship in Drosophila. However, existing approaches rely on rigid, rule-based algorithms and expensive hardware, limiting sensitivity to behavioral variations and accessibility. Here, we describe the DANCE (Drosophila Aggression and Courtship Evaluator), a low-cost, open-source platform combining machine learning-based classifiers and inexpensive hardware to quantify aggression and courtship. DANCE consists of six novel behavioral classifiers trained using a supervised machine learning algorithm. DANCE classifiers outperform existing rule-based algorithms by capturing dynamic behavioral variations. DANCE hardware is constructed using repurposed medicine blister packs and acrylic sheets, with recordings performed using smartphones, making it affordable and accessible. Benchmarking demonstrated that DANCE hardware performs comparably to sophisticated, high-cost setups. We validated DANCE in diverse contexts, including social isolation versus enrichment that modulate aggression and courtship, RNAi-mediated down-regulation of neuropeptide Dsk, and optogenetic silencing of dopaminergic neurons which promoted aggression. DANCE provides a cost-effective and portable solution for studying Drosophila behaviors in resource-limited settings or closer to natural habitats. Its accessibility and robust performance democratizes behavioral neuroscience, enabling rapid screening of genes and neuronal circuits underlying complex social behaviors.Competing Interest StatementRSPY, FA and PA are listed as inventors on a published patent related to this work by the Indian patent office, titled- Device For Measuring Complex Social Behaviors In Small Insects, Application No. 202441072884.
ISSN:2692-8205
DOI:10.1101/2025.01.03.631168
Fuente:Biological Science Database