NFIB influences progenitor competence in maturation of GABAergic neurons in mice

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Detalles Bibliográficos
Publicado en:	bioRxiv (Jan 8, 2025)
Autor principal:	Ann Rose Bright
Otros Autores:	Kotlyarenko, Yana, Neuhaus, Florian, Rodrigues, Diana, Chao, Feng, Peters, Christian, Vitali, Ilaria, Doenmez, Elif, Myoga, Michael H, Dvoretskova, Elena, Mayer, Christian
Publicado:	Cold Spring Harbor Laboratory Press
Materias:	National Federation of Independent Business Neural stem cells Embryos Neurons Neurogenesis Maturation Interneurons γ-Aminobutyric acid Telencephalon Chromatin remodeling Transcriptomics Progenitor cells Developmental stages
Acceso en línea:	Citation/Abstract Full text outside of ProQuest
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Descripción
Resumen:	Diverse types of GABAergic projection neurons and interneurons of the telencephalon derive from progenitors in a ventral germinal zone, called the ganglionic eminence. Using single-cell transcriptomics, chromatin accessibility profiling, lineage tracing, birthdating, heterochronic transplantation, and perturbation sequencing in mouse embryos, we investigated how progenitor competence influences the maturation and differentiation of these neurons. We found that the progression of neurogenesis over developmental time shapes maturation competence in ganglionic eminence progenitors, how they progress into mature states. In contrast, dierentiation competence, which defines the ability to produce diverse transcriptomic identities, remains largely unaffected by the stages of neurogenesis. Chromatin remodeling alongside a NFIB-driven regulatory gene module influences maturation competence in late-born neurons. These findings provide key insights into how transcriptional programs and chromatin accessibility govern neuronal maturation and the diversification of GABAergic neuron subtypes during neurodevelopment.Competing Interest StatementThe authors have declared no competing interest.Footnotes* The new version of the manuscript contains additional experiments, including CUT&RUN, in vivo overexpression, and tCROP-seq perturbation sequencing, providing functional validation and further supporting our conclusions on the role of NFIB in neuronal progenitor regulation.* http://141.5.108.55:3838/mind_shiny/
ISSN:	2692-8205
DOI:	10.1101/2024.03.18.585524
Fuente:	Biological Science Database