Massively parallel homogeneous amplification of chip-scale DNA for DNA information storage (MPHAC-DIS)
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| Pubblicato in: | Nature Communications vol. 16, no. 1 (2025), p. 667 |
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| Pubblicazione: |
Nature Publishing Group
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| Accesso online: | Citation/Abstract Full Text - PDF |
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| 022 | |a 2041-1723 | ||
| 024 | 7 | |a 10.1038/s41467-025-55986-9 |2 doi | |
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| 245 | 1 | |a Massively parallel homogeneous amplification of chip-scale DNA for DNA information storage (MPHAC-DIS) | |
| 260 | |b Nature Publishing Group |c 2025 | ||
| 513 | |a Journal Article | ||
| 520 | 3 | |a Chip scale DNA synthesis offers a high-throughput and cost-effective method for large-scale DNA-based information storage. Nevertheless, unbiased information retrieval from low-copy-number sequences remains a barricade that largely arises from the indispensable DNA amplification. Here, we devise a simulation-guided quantitative primer-template hybridization strategy to realize massively parallel homogeneous amplification of chip-scale DNA for DNA information storage (MPHAC-DIS). Using a fixed-energy primer design, we demonstrate the unbiasedness of MPHAC for amplifying 100,000-plex sequences. Simulations reveal that MPHAC achieves a fold-80 value of 1.0 compared to 3.2 with conventional fixed-length primers, lowering costs by up to four orders of magnitude through reduced over-sequencing. The MPHAC-DIS system using 35,406 encoded oligonucleotide allows simultaneous access of multimedia files including text, images, and videos with high decoding accuracy at very low sequencing depths. Specifically, even a ~ 1 × sequencing depth, with the combination of machine learning, results in an acceptable decoding accuracy of ~80%. The programmable and predictable MPHAC-DIS method thus opens new door for DNA-based large-scale data storage with potential industrial applications.Chip-scale DNA synthesis enables large-scale DNA data storage, but unbiased retrieval remains challenging. Here, authors introduce MPHAC-DIS, an energy-based amplification strategy enabling unbiased, high-accuracy DNA data retrieval, significantly reducing costs and enhancing data accessibility. | |
| 653 | |a Accuracy | ||
| 653 | |a Oligonucleotides | ||
| 653 | |a DNA biosynthesis | ||
| 653 | |a Industrial applications | ||
| 653 | |a Information storage | ||
| 653 | |a Gene sequencing | ||
| 653 | |a Information retrieval | ||
| 653 | |a Hybridization | ||
| 653 | |a Deoxyribonucleic acid--DNA | ||
| 653 | |a Data storage | ||
| 653 | |a Cost reduction | ||
| 653 | |a Information processing | ||
| 653 | |a Nucleotide sequence | ||
| 653 | |a Machine learning | ||
| 653 | |a Cost control | ||
| 653 | |a Synthesis | ||
| 653 | |a Data retrieval | ||
| 653 | |a Environmental | ||
| 773 | 0 | |t Nature Communications |g vol. 16, no. 1 (2025), p. 667 | |
| 786 | 0 | |d ProQuest |t Health & Medical Collection | |
| 856 | 4 | 1 | |3 Citation/Abstract |u https://www.proquest.com/docview/3155469352/abstract/embedded/H09TXR3UUZB2ISDL?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text - PDF |u https://www.proquest.com/docview/3155469352/fulltextPDF/embedded/H09TXR3UUZB2ISDL?source=fedsrch |