Scg2 drives reorganization of the corticospinal circuit with spinal premotor interneurons to recover motor function after stroke
Guardado en:
| Publicado en: | bioRxiv (Jan 22, 2025) |
|---|---|
| Autor principal: | |
| Otros Autores: | , , , , , , |
| Publicado: |
Cold Spring Harbor Laboratory Press
|
| Materias: | |
| Acceso en línea: | Citation/Abstract Full Text - PDF Full text outside of ProQuest |
| Etiquetas: |
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
MARC
| LEADER | 00000nab a2200000uu 4500 | ||
|---|---|---|---|
| 001 | 3158241900 | ||
| 003 | UK-CbPIL | ||
| 022 | |a 2692-8205 | ||
| 024 | 7 | |a 10.1101/2025.01.21.634186 |2 doi | |
| 035 | |a 3158241900 | ||
| 045 | 0 | |b d20250122 | |
| 100 | 1 | |a Sato, Tokiharu | |
| 245 | 1 | |a Scg2 drives reorganization of the corticospinal circuit with spinal premotor interneurons to recover motor function after stroke | |
| 260 | |b Cold Spring Harbor Laboratory Press |c Jan 22, 2025 | ||
| 513 | |a Working Paper | ||
| 520 | 3 | |a Brain injuries such as stroke damage neural circuitry and lead to functional deficits. Spared motor pathways are often reorganized and contribute to functional recovery; however, the connectivity and molecular mechanisms that drive the reorganization are largely unknown. Here, we demonstrate structural and functional connectivity reformed by spared corticospinal axons after stroke and determine a key secretory protein that drives the reorganization. We first found that corticospinal axons innervate specific areas of the denervated cervical cord after stroke. Anatomical and photometric analyses reveal that the axons reconnect to premotor V2a interneurons. Kinematic analyses of forelimb movements and chemogenetic silencing reveal their contribution to motor recovery. Translated mRNA expression analyses of V2a interneurons and astrocytes in the denervated cervical cord reveal diverse transcripts upregulated in the rewiring process. In particular, a secretory protein Scg2 is upregulated by injury-induced purinergic ATP signals and rehabilitative training-induced neural activity and possesses an ability to promote axon growth via cAMP and S6 signaling. Scg2 overexpression in the denervated cervical cord enhances axon rewiring, while the Scg2 knockdown attenuates it. The present data reveal the neural substrate and molecular mechanism essential to induce reorganization and recovery of the motor system, providing fundamental therapeutic targets for CNS injuries.Competing Interest StatementThe authors have declared no competing interest. | |
| 653 | |a Astrocytes | ||
| 653 | |a Molecular modelling | ||
| 653 | |a Brain injury | ||
| 653 | |a Gene expression | ||
| 653 | |a Structure-function relationships | ||
| 653 | |a Recovery of function | ||
| 653 | |a Interneurons | ||
| 653 | |a Therapeutic targets | ||
| 653 | |a Neural networks | ||
| 653 | |a Injuries | ||
| 653 | |a Stroke | ||
| 700 | 1 | |a Nakamura, Yuka | |
| 700 | 1 | |a Hoshina, Kana | |
| 700 | 1 | |a Inoue, Ken-Ichi | |
| 700 | 1 | |a Takada, Masahiko | |
| 700 | 1 | |a Yano, Masato | |
| 700 | 1 | |a Matsuzawa, Hitoshi | |
| 700 | 1 | |a Ueno, Masaki | |
| 773 | 0 | |t bioRxiv |g (Jan 22, 2025) | |
| 786 | 0 | |d ProQuest |t Biological Science Database | |
| 856 | 4 | 1 | |3 Citation/Abstract |u https://www.proquest.com/docview/3158241900/abstract/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text - PDF |u https://www.proquest.com/docview/3158241900/fulltextPDF/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |
| 856 | 4 | 0 | |3 Full text outside of ProQuest |u https://www.biorxiv.org/content/10.1101/2025.01.21.634186v1 |