Molecular Epidemiological Characteristics of Staphylococcus pseudintermedius, Staphylococcus coagulans, and Coagulase-Negative Staphylococci Cultured from Clinical Canine Skin and Ear Samples in Queensland
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| Publicado en: | Antibiotics vol. 14, no. 1 (2025), p. 80 |
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| Acceso en línea: | Citation/Abstract Full Text + Graphics Full Text - PDF |
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| 001 | 3159182292 | ||
| 003 | UK-CbPIL | ||
| 022 | |a 2079-6382 | ||
| 024 | 7 | |a 10.3390/antibiotics14010080 |2 doi | |
| 035 | |a 3159182292 | ||
| 045 | 2 | |b d20250101 |b d20251231 | |
| 084 | |a 231335 |2 nlm | ||
| 100 | 1 | |a Horsman, Sara |u School of Veterinary Science, The University of Queensland, Gatton, QLD 4343, Australia; <email>e.meler@uq.edu.au</email> (E.M.); <email>gibson.j@uq.edu.au</email> (J.S.G.) | |
| 245 | 1 | |a Molecular Epidemiological Characteristics of <i>Staphylococcus pseudintermedius</i>, <i>Staphylococcus coagulans,</i> and Coagulase-Negative Staphylococci Cultured from Clinical Canine Skin and Ear Samples in Queensland | |
| 260 | |b MDPI AG |c 2025 | ||
| 513 | |a Journal Article | ||
| 520 | 3 | |a Background/Objectives: Infections in dogs caused by methicillin-resistant staphylococci (MRS) present limited treatment options. This study’s objective was to investigate the molecular epidemiology of Staphylococcus spp. cultured exclusively from clinical canine skin and ear samples in Queensland, Australia, using whole-genome sequencing (WGS). Methods: Forty-two Staphylococcus spp. isolated from clinical canine skin and ear samples, from an unknown number of dogs, were sourced from two veterinary diagnostic laboratories between January 2022 and May 2023. These isolates underwent matrix-assisted laser desorption ionisation– time of flight bacterial identification, minimum inhibitory concentration testing using SensititreTM plates and WGS. Phylogenetic trees and core genome multilocus sequence typing (cgMLST) minimum spanning trees (MSTs) were constructed. Results: The isolates included methicillin-resistant and -sensitive S. pseudintermedius (MRSP: 57.1%, 24/42; and MSSP: 19.1%, 8/42), methicillin-resistant and -sensitive S. coagulans (MRSC: 14.3%, 6/42; and MSSC: 2.4%, 1/42) and methicillin-resistant coagulase-negative staphylococci (MR-CoNS: 7.1%, 3/42). Thirty-nine isolates were included after WGS, where all MRS harboured the mecA gene. Eighteen sequence types (STs) were identified, including three novel MRSP and six novel MSSP STs. MRSP ST496-V-VII (23%; 9/39) and MRSP ST749-IV-(IVg) (12.8%; 5/39) were commonly isolated. Phylogenetic analysis of single nucleotide polymorphisms showed that MRSP, MRSC and MSSC were similar to globally isolated staphylococci from canine skin and ear infections. Using cgMLST MSTs, MRSP isolates were not closely related to global strains. Conclusions: Our findings revealed a genotypically diverse geographical distribution and phylogenetic relatedness of staphylococci cultured from clinical canine skin and ear samples across Queensland. This highlights the importance of ongoing surveillance to aid in evidence-based treatment decisions and antimicrobial stewardship. | |
| 651 | 4 | |a Australia | |
| 651 | 4 | |a Queensland Australia | |
| 651 | 4 | |a United States--US | |
| 653 | |a Skin | ||
| 653 | |a Infections | ||
| 653 | |a Population | ||
| 653 | |a Geographical distribution | ||
| 653 | |a Phylogenetics | ||
| 653 | |a Epidemiology | ||
| 653 | |a Drug resistance | ||
| 653 | |a Laboratories | ||
| 653 | |a Methicillin | ||
| 653 | |a Nucleotide sequence | ||
| 653 | |a Penicillin | ||
| 653 | |a Genes | ||
| 653 | |a Phylogeny | ||
| 653 | |a Virulence | ||
| 653 | |a Dogs | ||
| 653 | |a Plasmids | ||
| 653 | |a Ear | ||
| 653 | |a Veterinary services | ||
| 653 | |a Nucleotides | ||
| 653 | |a MecA protein | ||
| 653 | |a Skin resistance | ||
| 653 | |a Gene sequencing | ||
| 653 | |a Graph theory | ||
| 653 | |a Minimum inhibitory concentration | ||
| 653 | |a Coagulase | ||
| 653 | |a Antimicrobial agents | ||
| 653 | |a Single-nucleotide polymorphism | ||
| 653 | |a Whole genome sequencing | ||
| 653 | |a Multilocus sequence typing | ||
| 653 | |a Microbiota | ||
| 653 | |a Evidence-based practice | ||
| 653 | |a Staphylococcus | ||
| 700 | 1 | |a Zaugg, Julian |u Australian Centre for Ecogenomics, School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia; <email>j.zaugg@uq.edu.au</email> | |
| 700 | 1 | |a Meler, Erika |u School of Veterinary Science, The University of Queensland, Gatton, QLD 4343, Australia; <email>e.meler@uq.edu.au</email> (E.M.); <email>gibson.j@uq.edu.au</email> (J.S.G.) | |
| 700 | 1 | |a Mikkelsen, Deirdre |u School of Agriculture and Food Sustainability, The University of Queensland, Brisbane, QLD 4072, Australia; <email>d.mikkelsen@uq.edu.au</email> | |
| 700 | 1 | |a Soares Magalhães, Ricardo J |u School of Veterinary Science, The University of Queensland, Gatton, QLD 4343, Australia; <email>e.meler@uq.edu.au</email> (E.M.); <email>gibson.j@uq.edu.au</email> (J.S.G.) | |
| 700 | 1 | |a Gibson, Justine S |u School of Veterinary Science, The University of Queensland, Gatton, QLD 4343, Australia; <email>e.meler@uq.edu.au</email> (E.M.); <email>gibson.j@uq.edu.au</email> (J.S.G.) | |
| 773 | 0 | |t Antibiotics |g vol. 14, no. 1 (2025), p. 80 | |
| 786 | 0 | |d ProQuest |t Biological Science Database | |
| 856 | 4 | 1 | |3 Citation/Abstract |u https://www.proquest.com/docview/3159182292/abstract/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text + Graphics |u https://www.proquest.com/docview/3159182292/fulltextwithgraphics/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text - PDF |u https://www.proquest.com/docview/3159182292/fulltextPDF/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |