Investigating Ultrafiltration Membranes and Operation Modes for Improved Lentiviral Vector Processing
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| Udgivet i: | Engineering in Life Sciences vol. 25, no. 1 (Jan 1, 2025) |
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John Wiley & Sons, Inc.
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| Online adgang: | Citation/Abstract Full Text Full Text - PDF |
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| LEADER | 00000nab a2200000uu 4500 | ||
|---|---|---|---|
| 001 | 3161305872 | ||
| 003 | UK-CbPIL | ||
| 022 | |a 1618-2863 | ||
| 024 | 7 | |a 10.1002/elsc.202400057 |2 doi | |
| 035 | |a 3161305872 | ||
| 045 | 0 | |b d20250101 | |
| 100 | 1 | |a Labisch, Jennifer J. |u Lab Essentials Applications Development, Sartorius, Göttingen, Germany | |
| 245 | 1 | |a Investigating Ultrafiltration Membranes and Operation Modes for Improved Lentiviral Vector Processing | |
| 260 | |b John Wiley & Sons, Inc. |c Jan 1, 2025 | ||
| 513 | |a Journal Article | ||
| 520 | 3 | |a ABSTRACT The demand for lentiviral vectors (LVs) as tools for ex vivo gene therapies is ever‐increasing. Despite their promising applications, challenges in LV production remain largely due to the fragile envelope, which challenges the maintenance of vector stability. Thus, downstream processing optimization to enhance efficiency, yield, and product quality is necessary. This study investigated the influence of membrane types and filtration devices during ultrafiltration (UF). Nine different membrane materials consisting of polyethersulfone (PES), regenerated cellulose, or Hydrosart, with distinct molecular weight cutoffs, were evaluated in stirred cells, centrifugal ultrafilters, and crossflow cassettes. The evaluation was based on the ability to retain infectious LV particles and remove impurities. The analysis revealed that a reinforced 100 kDa PES and a 300 kDa Hydrosart membrane had the best overall ability to concentrate infectious LVs and remove DNA, especially when operated in a stirred cell. Challenges were seen in the nonoptimized crossflow cassette process, where infectious LV recovery was generally lower compared to other devices. We demonstrated that membrane material and filtration device have a direct impact on the efficiency of LV UF. | |
| 651 | 4 | |a Germany | |
| 653 | |a Membranes | ||
| 653 | |a Tensile strength | ||
| 653 | |a Product quality | ||
| 653 | |a Cross flow | ||
| 653 | |a Cellulose | ||
| 653 | |a Experiments | ||
| 653 | |a Impurities | ||
| 653 | |a Gene therapy | ||
| 653 | |a Expression vectors | ||
| 653 | |a Polyethersulfones | ||
| 653 | |a Vector processing (computers) | ||
| 653 | |a Molecular weight | ||
| 653 | |a Chromatography | ||
| 653 | |a Cassettes | ||
| 653 | |a Manufacturing | ||
| 653 | |a Ultrafiltration | ||
| 653 | |a Vectors (Biology) | ||
| 653 | |a Filtration | ||
| 653 | |a Proteins | ||
| 700 | 1 | |a Evangelopoulou, Maria |u Lab Essentials Applications Development, Sartorius, Göttingen, Germany | |
| 700 | 1 | |a Schleuß, Tobias |u Ultrafiltration Membrane Technology, Sartorius, Göttingen, Germany | |
| 700 | 1 | |a Pickl, Andreas |u Lab Essentials Applications Development, Sartorius, Göttingen, Germany | |
| 773 | 0 | |t Engineering in Life Sciences |g vol. 25, no. 1 (Jan 1, 2025) | |
| 786 | 0 | |d ProQuest |t Engineering Database | |
| 856 | 4 | 1 | |3 Citation/Abstract |u https://www.proquest.com/docview/3161305872/abstract/embedded/L8HZQI7Z43R0LA5T?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text |u https://www.proquest.com/docview/3161305872/fulltext/embedded/L8HZQI7Z43R0LA5T?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text - PDF |u https://www.proquest.com/docview/3161305872/fulltextPDF/embedded/L8HZQI7Z43R0LA5T?source=fedsrch |