Somatic mutations distinguish melanocyte subpopulations in human skin
-д хадгалсан:
| -д хэвлэсэн: | bioRxiv (Feb 8, 2025) |
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| Үндсэн зохиолч: | |
| Бусад зохиолчид: | , , , , , , , , , , , , , |
| Хэвлэсэн: |
Cold Spring Harbor Laboratory Press
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| Нөхцлүүд: | |
| Онлайн хандалт: | Citation/Abstract Full text outside of ProQuest |
| Шошгууд: |
Шошго байхгүй, Энэхүү баримтыг шошголох эхний хүн болох!
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MARC
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| 001 | 3165216992 | ||
| 003 | UK-CbPIL | ||
| 022 | |a 2692-8205 | ||
| 024 | 7 | |a 10.1101/2025.02.07.637114 |2 doi | |
| 035 | |a 3165216992 | ||
| 045 | 0 | |b d20250208 | |
| 100 | 1 | |a Tandukar, Bishal | |
| 245 | 1 | |a Somatic mutations distinguish melanocyte subpopulations in human skin | |
| 260 | |b Cold Spring Harbor Laboratory Press |c Feb 8, 2025 | ||
| 513 | |a Working Paper | ||
| 520 | 3 | |a To better understand the homeostatic mechanisms governing melanocytes, we performed deep phenotyping of clonal expansions of single melanocytes from human skin. In total, we interrogated the mutational landscapes, gene expression profiles, and morphological features of 297 melanocytes from 31 donors. To our surprise, a population of melanocytes with low mutation burden was maintained in sun damaged skin. These melanocytes were more stem-like, smaller, less dendritic and displayed distinct gene expression profiles compared to their counterparts with high mutation burdens. We used single-cell spatial transcriptomics (10X Xenium) to reveal the spatial distribution of melanocytes inferred to have low and high mutation burdens (LowMut and HighMut cells), based on their gene expression profiles. LowMut melanocytes were found in hair follicles as well as in the interfollicular epidermis, whereas HighMut melanocytes resided almost exclusively in the interfollicular epidermis. We propose that melanocytes in the hair follicle occupy a privileged niche, protected from UV radiation, but periodically migrate out of the hair follicle to replenish the interfollicular epidermis after waves of photodamage. More broadly, our study illustrates the advantages of a cell atlas that includes mutational information, as cells can change their cellular states and positional coordinates over time, but mutations are like scars, providing a historical record of the homeostatic processes that were operative on each cell.Competing Interest StatementThe authors have declared no competing interest. | |
| 653 | |a Melanocytes | ||
| 653 | |a Follicles | ||
| 653 | |a Gene expression | ||
| 653 | |a Spatial distribution | ||
| 653 | |a Transcriptomics | ||
| 653 | |a Skin | ||
| 653 | |a Phenotyping | ||
| 653 | |a Epidermis | ||
| 653 | |a Mutation | ||
| 653 | |a Ultraviolet radiation | ||
| 700 | 1 | |a Deivendran, Delahny | |
| 700 | 1 | |a Chen, Limin | |
| 700 | 1 | |a Bahrani, Neda | |
| 700 | 1 | |a Weier, Beatrice | |
| 700 | 1 | |a Sharma, Harsh | |
| 700 | 1 | |a Cruz-Pacheco, Noel | |
| 700 | 1 | |a Hu, Min | |
| 700 | 1 | |a Marks, Kayla | |
| 700 | 1 | |a Zitnay, Rebecca G | |
| 700 | 1 | |a Bandari, Aravind K | |
| 700 | 1 | |a Rojina Nekoonam | |
| 700 | 1 | |a Yeh, Iwei | |
| 700 | 1 | |a Judson-Torres, Robert | |
| 700 | 1 | |a A Hunter Shain | |
| 773 | 0 | |t bioRxiv |g (Feb 8, 2025) | |
| 786 | 0 | |d ProQuest |t Biological Science Database | |
| 856 | 4 | 1 | |3 Citation/Abstract |u https://www.proquest.com/docview/3165216992/abstract/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |
| 856 | 4 | 0 | |3 Full text outside of ProQuest |u https://www.biorxiv.org/content/10.1101/2025.02.07.637114v1 |