Slika omota

Polyhydroxybutyrate as a Novel Biopolymer for Dental Restorative Materials: Biological and Morphological Analysis

Spremljeno u:
Izdano u:Polymers vol. 17, no. 3 (2025), p. 313
Glavni autor: Ozkaya, Cigdem Atalayin
Daljnji autori: Ertan, Beliz, Gizem Kaftan Ocal, Armagan, Guliz, Gungor, Gokhan, Demirbilek, Murat, Tezel, Huseyin, Notaro, Vincenzo, Scotti, Nicola, Baldi, Andrea
Izdano:
MDPI AG
Teme:
3M ESPE Food & Drug Administration > FDA
Turkey
United States > US
Belgium
Tissue engineering
Biocompatibility
Software
Transplants & implants
Toxicity
Morphology
Cytotoxicity
Polyhydroxybutyrate
Dental materials
Raw materials
Biopolymers
Biological activity
Composite materials
Cell culture
Scanning electron microscopy
Statistical analysis
Fermentation
Polymers
Fibroblasts
FDA approval
Assaying
Resins
Computed tomography
Toxicity testing
Dentistry
Membranes
Online pristup:Citation/Abstract
Full Text + Graphics
Full Text - PDF
Oznake: Dodaj oznaku
Bez oznaka, Budi prvi tko označuje ovaj zapis!
Sažetak:Polyhydroxybutyrate (PHB) is a biopolymer produced by bacteria. This study aimed to implement the production process of experimental medical-grade PHB and to evaluate its morphology and biocompatibility compared to conventional resin-based composites (RBCs). PHB raw material was produced via biological process and then the membrane was generated via electrospinning specifically for this study and imaged with Micro-Computed Tomography (Micro-CT) and scanning electron microscopy (SEM). MTS assay was used to assess the cytotoxicity of PHB compared to other materials. Test groups included two packable resin composites (Point 4-Kerr, G-aenial anterior-GC), two flowable resin composites (Filtek Ultimate Flowable-3M ESPE, Nova Compo HF-Imicryl), a compomer (Nova Compomer-Imicryl), a fissure-sealant (Fissured Nova Plus-Imicryl), and the PHB membrane (Innovaplast Biotechnology Inc., Eskisehir, Turkey). A control group consisting of cells without any test material was also produced. To perform the MTS assay, disc-shaped specimens of the aforementioned materials were prepared and then incubated with mouse fibroblast cells (L929) for 24 and 48 h. Micro-CT and SEM images revealed a homogeneous and fibrillary structure of the PHB. MTS assay revealed the highest cell viability in the PHB, Nova Compomer, and Fissured Nova Plus groups after 24 h. PHB and Nova Compomer showed the highest viability rates at 48 h while other RBCs had rates below 25% (p < 0.05). Considering the cell viability data and its fibrillary structure, from a biological point of view, PHB seems a promising biopolymer that might have applications in the field of dental restorative materials.
ISSN:2073-4360
Digitalni identifikator objekta:10.3390/polym17030313
Izvor:Materials Science Database