Stimulation of the Caulobacter crescentus surface sensing pathway by deletion of a specialized minor pilin-like gene
Gorde:
| Argitaratua izan da: | bioRxiv (Feb 12, 2025) |
|---|---|
| Egile nagusia: | |
| Beste egile batzuk: | , , |
| Argitaratua: |
Cold Spring Harbor Laboratory Press
|
| Gaiak: | |
| Sarrera elektronikoa: | Citation/Abstract Full text outside of ProQuest |
| Etiketak: |
Etiketarik gabe, Izan zaitez lehena erregistro honi etiketa jartzen!
|
MARC
| LEADER | 00000nab a2200000uu 4500 | ||
|---|---|---|---|
| 001 | 3165948627 | ||
| 003 | UK-CbPIL | ||
| 022 | |a 2692-8205 | ||
| 024 | 7 | |a 10.1101/2025.02.12.637803 |2 doi | |
| 035 | |a 3165948627 | ||
| 045 | 0 | |b d20250212 | |
| 100 | 1 | |a Farah Obeid Charrouf | |
| 245 | 1 | |a Stimulation of the Caulobacter crescentus surface sensing pathway by deletion of a specialized minor pilin-like gene | |
| 260 | |b Cold Spring Harbor Laboratory Press |c Feb 12, 2025 | ||
| 513 | |a Working Paper | ||
| 520 | 3 | |a Bacteria colonize surfaces through complex mechanisms of surface sensing. Pili are dynamic bacterial appendages that play an important role in this process. In Caulobacter crescentus, tension on retracting, surface-bound pili triggers the rapid synthesis of the adhesive holdfast, which permanently attaches cells to surfaces. However, the detailed mechanisms of pilus-mediated surface sensing are unclear. In this study, we used a genetic screen to isolate mutants with altered pilus activity to identify genes that may be involved in pilus-mediated surface-sensing. This screen identified cpaL, whose deletion led to reduced piliation levels, and surprisingly, a threefold increase in surface adhesion due to increased holdfast production. To understand this finding, we compared holdfast synthesis in wild-type and cpaL mutant cells under conditions that block pilus retraction. While this treatment increased holdfast production in wild-type cells by triggering the surface-sensing pathway, no increase was observed in the cpaL mutant, suggesting that mutation of cpaL maximally stimulates surface-sensing. Furthermore, when the cpaL mutant was grown in a medium that blocks the surface sensing pathway, cells exhibited decreased surface attachment and holdfast production, consistent with a role for CpaL in pilus-dependent surface sensing in C. crescentus. To better understand the function of CpaL, we analyzed its predicted structure, which suggested that CpaL is a minor pilin fused to a mechanosensitive von Willebrand factor type A (vWA) domain that could be accommodated at the pilus tip. These results collectively position CpaL as a strong candidate for a mechanosensory element in pilus-mediated surface sensing. | |
| 653 | |a Pilin | ||
| 653 | |a Von Willebrand factor | ||
| 653 | |a Mutants | ||
| 653 | |a Structure-function relationships | ||
| 653 | |a Gene deletion | ||
| 653 | |a Genetic screening | ||
| 653 | |a Pili | ||
| 653 | |a Caulobacter crescentus | ||
| 700 | 1 | |a Whitfield, Gregory B | |
| 700 | 1 | |a Ellison, Courtney K | |
| 700 | 1 | |a Brun, Yves V | |
| 773 | 0 | |t bioRxiv |g (Feb 12, 2025) | |
| 786 | 0 | |d ProQuest |t Biological Science Database | |
| 856 | 4 | 1 | |3 Citation/Abstract |u https://www.proquest.com/docview/3165948627/abstract/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |
| 856 | 4 | 0 | |3 Full text outside of ProQuest |u https://www.biorxiv.org/content/10.1101/2025.02.12.637803v1 |