An archaeal CBASS system eliminates viruses without killing the host cells
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| Publicado en: | bioRxiv (Feb 13, 2025) |
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| Otros Autores: | , , , , , |
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Cold Spring Harbor Laboratory Press
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| Acceso en línea: | Citation/Abstract Full text outside of ProQuest |
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|---|---|---|---|
| 001 | 3166351656 | ||
| 003 | UK-CbPIL | ||
| 022 | |a 2692-8205 | ||
| 024 | 7 | |a 10.1101/2024.09.12.612678 |2 doi | |
| 035 | |a 3166351656 | ||
| 045 | 0 | |b d20250213 | |
| 100 | 1 | |a Choudhary, Deepak Kumar | |
| 245 | 1 | |a An archaeal CBASS system eliminates viruses without killing the host cells | |
| 260 | |b Cold Spring Harbor Laboratory Press |c Feb 13, 2025 | ||
| 513 | |a Working Paper | ||
| 520 | 3 | |a Many cyclic-oligonucleotide-based antiphage signalling systems (CBASS) defend against viral infections using a TIR-SAVED domain protein that depletes cellular NAD+ levels, eventually leading to cell dormancy or death. This abortive infection strategy is beneficial in stopping fast lytic infections, as cells die before spreading the virus to neighboring cells. However, many archaea are infected by chronic "temperate" viruses that coexist with their hosts for extended periods. In such situations, abortive infection could be detrimental, as the cost of immunity may outweigh that of infection. In this study, we examine an archaeal Type II-C CBASS system from Haloferax strain Atlit 48N that was heterologously expressed in the model organism Haloferax volcanii DS2. We demonstrate that this system protects against a chronically infecting virus, HFPV-1, and enables clearing of the virus after several passages without killing the host. Moreover, cells that have cleared the virus become substantially more resistant to future HFPV-1 infections, without acquiring CRISPR spacers from that virus. Cell death during viral infection only occurs after extensive incubation with HFPV-1. These findings suggest that CBASS could be beneficial even for archaea exposed to chronic infecting viruses, potentially explaining why such systems are relatively common in archaea.Competing Interest StatementThe authors have declared no competing interest.Footnotes* Author contribution section added, this is now required by most journals. | |
| 653 | |a Infections | ||
| 653 | |a Chronic infection | ||
| 653 | |a Cell death | ||
| 653 | |a Viruses | ||
| 653 | |a Oligonucleotides | ||
| 653 | |a Dormancy | ||
| 653 | |a Viral infections | ||
| 653 | |a CRISPR | ||
| 700 | 1 | |a Singla, Himani | |
| 700 | 1 | |a Vassover, Dana | |
| 700 | 1 | |a Golan, Noam | |
| 700 | 1 | |a Reshef, Leah | |
| 700 | 1 | |a Hadar Shukrun | |
| 700 | 1 | |a Gophna, Uri | |
| 773 | 0 | |t bioRxiv |g (Feb 13, 2025) | |
| 786 | 0 | |d ProQuest |t Biological Science Database | |
| 856 | 4 | 1 | |3 Citation/Abstract |u https://www.proquest.com/docview/3166351656/abstract/embedded/75I98GEZK8WCJMPQ?source=fedsrch |
| 856 | 4 | 0 | |3 Full text outside of ProQuest |u https://www.biorxiv.org/content/10.1101/2024.09.12.612678v2 |