Plasticity of Expression of Stem Cell and EMT Markers in Breast Cancer Cells in 2D and 3D Culture Depend on the Spatial Parameters of Cell Growth; Mathematical Modeling of Mechanical Stress in Cell Culture in Relation to ECM Stiffness
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| Udgivet i: | Bioengineering vol. 12, no. 2 (2025), p. 147 |
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| Hovedforfatter: | |
| Andre forfattere: | , , , , , , , |
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MDPI AG
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| Online adgang: | Citation/Abstract Full Text + Graphics Full Text - PDF |
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| 022 | |a 2306-5354 | ||
| 024 | 7 | |a 10.3390/bioengineering12020147 |2 doi | |
| 035 | |a 3170946351 | ||
| 045 | 2 | |b d20250101 |b d20251231 | |
| 100 | 1 | |a Szostakowska-Rodzoś, Małgorzata |u Molecular and Translational Oncology Department, Maria Sklodowska-Curie National Research Institute of Oncology, Roentgena 5, 02-781 Warsaw, Poland; <email>malgorzata.szostakowska-rodzos@nio.gov.pl</email> (M.S.-R.); <email>mateusz.chmielarczyk@nio.gov.pl</email> (M.C.); <email>weronika.juras97@gmail.com</email> (W.Z.); <email>anna.fabisiewicz@nio.gov.pl</email> (A.F.); <email>agata.kurzyk@nio.gov.pl</email> (A.K.); <email>izabella.mysliwy@nio.gov.pl</email> (I.M.); | |
| 245 | 1 | |a Plasticity of Expression of Stem Cell and EMT Markers in Breast Cancer Cells in 2D and 3D Culture Depend on the Spatial Parameters of Cell Growth; Mathematical Modeling of Mechanical Stress in Cell Culture in Relation to ECM Stiffness | |
| 260 | |b MDPI AG |c 2025 | ||
| 513 | |a Journal Article | ||
| 520 | 3 | |a The majority of the current cancer research is based on two-dimensional cell cultures and animal models. These methods have limitations, including different expressions of key factors involved in carcinogenesis and metastasis, depending on culture conditions. Addressing these differences is crucial in obtaining physiologically relevant models. In this manuscript we analyzed the plasticity of the expression of stem cell and epithelial/mesenchymal markers in breast cancer cells, depending on culture conditions. Significant differences in marker expression were observed in different growth models not only between 2D and 3D conditions but also between two different 3D models. Differences observed in the levels of adherent junction protein E-cadherin in two different 3D models suggest that spatial parameters of cell growth and physical stress in the culture may affect the expression of junction proteins. To provide an explanation of this phenomenon on the grounds of mechanobiology, these parameters were analyzed using a mathematical model of the 3D bioprinted cell culture. The finite element mechanical model generated in this study includes an extracellular matrix and a group of regularly placed cells. The single-cell model comprises an idealized cytoskeleton, cortex, cytoplasm, and nucleus. The analysis of the model revealed that the stress generated by external pressure is transferred between the cells, generating specific stress fields, depending on growth conditions. We have analyzed and compared stress fields in two different growth conditions, each corresponding to a different elasticity of extracellular matrix. We have demonstrated that soft matrix conditions produce more stress than a stiff matrix in the single cell as well as in cellular spheroids. The observed differences can explain the plasticity of E-cadherin expression in response to mechanical stress. These results should contribute to a better understanding of the differences between various growth models. | |
| 651 | 4 | |a United States--US | |
| 651 | 4 | |a Germany | |
| 653 | |a External pressure | ||
| 653 | |a Plastic properties | ||
| 653 | |a Spheroids | ||
| 653 | |a Mathematical analysis | ||
| 653 | |a Metastasis | ||
| 653 | |a Antibodies | ||
| 653 | |a Mathematical models | ||
| 653 | |a Carcinogens | ||
| 653 | |a Cell culture | ||
| 653 | |a Cell growth | ||
| 653 | |a Drug testing | ||
| 653 | |a Stem cells | ||
| 653 | |a Cytoskeleton | ||
| 653 | |a Breast cancer | ||
| 653 | |a Metastases | ||
| 653 | |a Animal models | ||
| 653 | |a Extracellular matrix | ||
| 653 | |a Carcinogenesis | ||
| 653 | |a Stress distribution | ||
| 653 | |a Proteins | ||
| 653 | |a Gene expression | ||
| 653 | |a Cancer research | ||
| 653 | |a Growth conditions | ||
| 653 | |a Three dimensional models | ||
| 653 | |a Medical research | ||
| 653 | |a E-cadherin | ||
| 653 | |a Cytoplasm | ||
| 653 | |a Parameters | ||
| 653 | |a Plastic foam | ||
| 653 | |a Hydrogels | ||
| 653 | |a Plasticity | ||
| 653 | |a Physical stress | ||
| 700 | 1 | |a Chmielarczyk, Mateusz |u Molecular and Translational Oncology Department, Maria Sklodowska-Curie National Research Institute of Oncology, Roentgena 5, 02-781 Warsaw, Poland; <email>malgorzata.szostakowska-rodzos@nio.gov.pl</email> (M.S.-R.); <email>mateusz.chmielarczyk@nio.gov.pl</email> (M.C.); <email>weronika.juras97@gmail.com</email> (W.Z.); <email>anna.fabisiewicz@nio.gov.pl</email> (A.F.); <email>agata.kurzyk@nio.gov.pl</email> (A.K.); <email>izabella.mysliwy@nio.gov.pl</email> (I.M.); | |
| 700 | 1 | |a Zacharska, Weronika |u Molecular and Translational Oncology Department, Maria Sklodowska-Curie National Research Institute of Oncology, Roentgena 5, 02-781 Warsaw, Poland; <email>malgorzata.szostakowska-rodzos@nio.gov.pl</email> (M.S.-R.); <email>mateusz.chmielarczyk@nio.gov.pl</email> (M.C.); <email>weronika.juras97@gmail.com</email> (W.Z.); <email>anna.fabisiewicz@nio.gov.pl</email> (A.F.); <email>agata.kurzyk@nio.gov.pl</email> (A.K.); <email>izabella.mysliwy@nio.gov.pl</email> (I.M.); | |
| 700 | 1 | |a Fabisiewicz, Anna |u Molecular and Translational Oncology Department, Maria Sklodowska-Curie National Research Institute of Oncology, Roentgena 5, 02-781 Warsaw, Poland; <email>malgorzata.szostakowska-rodzos@nio.gov.pl</email> (M.S.-R.); <email>mateusz.chmielarczyk@nio.gov.pl</email> (M.C.); <email>weronika.juras97@gmail.com</email> (W.Z.); <email>anna.fabisiewicz@nio.gov.pl</email> (A.F.); <email>agata.kurzyk@nio.gov.pl</email> (A.K.); <email>izabella.mysliwy@nio.gov.pl</email> (I.M.); | |
| 700 | 1 | |a Kurzyk, Agata |u Molecular and Translational Oncology Department, Maria Sklodowska-Curie National Research Institute of Oncology, Roentgena 5, 02-781 Warsaw, Poland; <email>malgorzata.szostakowska-rodzos@nio.gov.pl</email> (M.S.-R.); <email>mateusz.chmielarczyk@nio.gov.pl</email> (M.C.); <email>weronika.juras97@gmail.com</email> (W.Z.); <email>anna.fabisiewicz@nio.gov.pl</email> (A.F.); <email>agata.kurzyk@nio.gov.pl</email> (A.K.); <email>izabella.mysliwy@nio.gov.pl</email> (I.M.); | |
| 700 | 1 | |a Myśliwy, Izabella |u Molecular and Translational Oncology Department, Maria Sklodowska-Curie National Research Institute of Oncology, Roentgena 5, 02-781 Warsaw, Poland; <email>malgorzata.szostakowska-rodzos@nio.gov.pl</email> (M.S.-R.); <email>mateusz.chmielarczyk@nio.gov.pl</email> (M.C.); <email>weronika.juras97@gmail.com</email> (W.Z.); <email>anna.fabisiewicz@nio.gov.pl</email> (A.F.); <email>agata.kurzyk@nio.gov.pl</email> (A.K.); <email>izabella.mysliwy@nio.gov.pl</email> (I.M.); | |
| 700 | 1 | |a Kozaryna, Zofia |u Molecular and Translational Oncology Department, Maria Sklodowska-Curie National Research Institute of Oncology, Roentgena 5, 02-781 Warsaw, Poland; <email>malgorzata.szostakowska-rodzos@nio.gov.pl</email> (M.S.-R.); <email>mateusz.chmielarczyk@nio.gov.pl</email> (M.C.); <email>weronika.juras97@gmail.com</email> (W.Z.); <email>anna.fabisiewicz@nio.gov.pl</email> (A.F.); <email>agata.kurzyk@nio.gov.pl</email> (A.K.); <email>izabella.mysliwy@nio.gov.pl</email> (I.M.); | |
| 700 | 1 | |a Postek, Eligiusz |u Institute of Fundamental Technological Research, Polish Academy of Sciences, Pawińskiego St. 5B, 02-106 Warsaw, Poland; <email>epostek@ippt.pan.pl</email> | |
| 700 | 1 | |a Grzybowska, Ewa A |u Molecular and Translational Oncology Department, Maria Sklodowska-Curie National Research Institute of Oncology, Roentgena 5, 02-781 Warsaw, Poland; <email>malgorzata.szostakowska-rodzos@nio.gov.pl</email> (M.S.-R.); <email>mateusz.chmielarczyk@nio.gov.pl</email> (M.C.); <email>weronika.juras97@gmail.com</email> (W.Z.); <email>anna.fabisiewicz@nio.gov.pl</email> (A.F.); <email>agata.kurzyk@nio.gov.pl</email> (A.K.); <email>izabella.mysliwy@nio.gov.pl</email> (I.M.); | |
| 773 | 0 | |t Bioengineering |g vol. 12, no. 2 (2025), p. 147 | |
| 786 | 0 | |d ProQuest |t Engineering Database | |
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