The Optimization of Demulsification Using Composite Fatty Acids in Aqueous Enzymatic Extraction and the Changes of the Emulsion Stability During Demulsification

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Udgivet i:Foods vol. 14, no. 5 (2025), p. 749
Hovedforfatter: Shao, Zhihua
Andre forfattere: Kong, Xiangrui, Yang, Hanxiang, Zhang, Yiyang, Yang, Chenxian, Chen, Fusheng, Wang, Zikun, Chen, Jiaxun, Zhu, Tingwei, Ying, Xin, Chen, Yu
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LEADER 00000nab a2200000uu 4500
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022 |a 2304-8158 
024 7 |a 10.3390/foods14050749  |2 doi 
035 |a 3176386943 
045 2 |b d20250301  |b d20250314 
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100 1 |a Shao, Zhihua 
245 1 |a The Optimization of Demulsification Using Composite Fatty Acids in Aqueous Enzymatic Extraction and the Changes of the Emulsion Stability During Demulsification 
260 |b MDPI AG  |c 2025 
513 |a Journal Article 
520 3 |a Aqueous enzymatic extraction (AEE) can simultaneously separate oil and protein. However, a stable O/W emulsion is present in the AEE process, which is not favorable for extracting oils. This study optimized the use of heptanoic and octanoic acids for demulsification in aqueous enzymatic extraction. The optimal condition for demulsification, including a fatty acid ratio of 1:3 (heptanoic acid to octanoic acid) with 1.00% addition, a reaction time of 40 min, a temperature of 70 °C, and a solid-to-liquid ratio of 1:5, resulted in a demulsification rate of 97.95% ± 0.03%. After demulsification, the particle size of the peanut emulsion increased, while the absolute potential value and conductivity decreased. The type and content of proteins decreased, and the tertiary structure also changed, with tryptophan residues buried within the proteins, shifting the system from a polar to nonpolar environment. The microstructure of the emulsion changed and the emulsion transformed into W/O. To summarize, composite fatty acid had a significant effect on the demulsification of emulsion. 
651 4 |a Shanghai China 
651 4 |a China 
653 |a Oil recovery 
653 |a Tryptophan 
653 |a Fatty acids 
653 |a Ratios 
653 |a Optimization 
653 |a Emulsions 
653 |a Environmental impact 
653 |a Metabolism 
653 |a Buried structures 
653 |a Oils & fats 
653 |a Proteins 
653 |a Octanoic acid 
653 |a Tertiary structure 
653 |a Temperature 
653 |a Methods 
653 |a Oilseeds 
653 |a Protein structure 
653 |a Sedimentation & deposition 
653 |a Enzymes 
653 |a Peanuts 
700 1 |a Kong, Xiangrui 
700 1 |a Yang, Hanxiang 
700 1 |a Zhang, Yiyang 
700 1 |a Yang, Chenxian 
700 1 |a Chen, Fusheng 
700 1 |a Wang, Zikun 
700 1 |a Chen, Jiaxun 
700 1 |a Zhu, Tingwei 
700 1 |a Ying, Xin 
700 1 |a Chen, Yu 
773 0 |t Foods  |g vol. 14, no. 5 (2025), p. 749 
786 0 |d ProQuest  |t Agriculture Science Database 
856 4 1 |3 Citation/Abstract  |u https://www.proquest.com/docview/3176386943/abstract/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch 
856 4 0 |3 Full Text + Graphics  |u https://www.proquest.com/docview/3176386943/fulltextwithgraphics/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch 
856 4 0 |3 Full Text - PDF  |u https://www.proquest.com/docview/3176386943/fulltextPDF/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch