DNA metabarcoding of non-fungal eukaryotic diversity in air and snow of Livingston Island, South Shetland Islands, Antarctica

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Argitaratua izan da:Polar Research vol. 44 (2025)
Egile nagusia: Paulo E.A.S. Câmara
Beste egile batzuk: Tina Šantl‑Temkiv, Otavio H.P. Pinto, Convey, Peter, Manuel Dall’Osto, Fabio L.V. Bones, Carvalho-Silva, Micheline, Diego Knop Henriques, Rosa, Luiz Henrique
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024 7 |a 10.33265/polar.v44.8293  |2 doi 
035 |a 3177467910 
045 2 |b d20250101  |b d20251231 
084 |a 211036  |2 nlm 
100 1 |a Paulo E.A.S. Câmara 
245 1 |a DNA metabarcoding of non-fungal eukaryotic diversity in air and snow of Livingston Island, South Shetland Islands, Antarctica 
260 |b Open Academia  |c 2025 
513 |a Journal Article 
520 3 |a A major natural route of dispersal to Antarctica is often assumed to be atmospheric transport, although few studies have documented this in detail. Aerial dispersal to Antarctica is very challenging as the continent is geographically remote from other land areas and is isolated by the atmospheric circumpolar vortex. Detailed information about aerial routes by which microorganisms arrive and circulate in Antarctica is generally lacking, as few aerobiological studies have focused on eukaryotes and those that have predominantly relied on traditional morphological identification. Recent advances in molecular biology, such as DNA metabarcoding by high throughput sequencing (HTS), have provided a powerful new tool for the study of atmospheric biological diversity and can retrieve levels of diversity an order of magnitude higher than traditional methods. In this study, we used HTS to investigate the diversity of non-fungal eukaryotes present in the atmosphere and freshly precipitated snow on Livingston Island. In a total of 740 m3 of air and 3.76 L of snow sampled, representatives of four kingdoms (Protozoa, Chromista, Viridiplantae and Animalia) and five phyla (Ciliophora, Ochrophyta, Chlorophyta, Magnoliophyta and Porifera) were found. The most diverse phylum was Chlorophyta, represented in our samples by 10 taxa, with Trebouxia asymmetrica Friedl & Gärtner the most abundant representative. 
651 4 |a South Shetland Islands 
651 4 |a Livingston Island 
651 4 |a Brazil 
651 4 |a Antarctica 
653 |a Fungi 
653 |a Flowers & plants 
653 |a Snow 
653 |a Molecular biology 
653 |a Eukaryotes 
653 |a Protozoa 
653 |a Ribosomal DNA 
653 |a Organisms 
653 |a Eukaryota 
653 |a Islands 
653 |a Marine invertebrates 
653 |a Next-generation sequencing 
653 |a Gene sequencing 
653 |a DNA barcoding 
653 |a Deoxyribonucleic acid--DNA 
653 |a Databases 
653 |a Taxonomy 
653 |a Biodiversity 
653 |a DNA 
653 |a Microorganisms 
653 |a Climate change 
653 |a Chlorophyta 
653 |a Environmental 
700 1 |a Tina Šantl‑Temkiv 
700 1 |a Otavio H.P. Pinto 
700 1 |a Convey, Peter 
700 1 |a Manuel Dall’Osto 
700 1 |a Fabio L.V. Bones 
700 1 |a Carvalho-Silva, Micheline 
700 1 |a Diego Knop Henriques 
700 1 |a Rosa, Luiz Henrique 
773 0 |t Polar Research  |g vol. 44 (2025) 
786 0 |d ProQuest  |t Engineering Database 
856 4 1 |3 Citation/Abstract  |u https://www.proquest.com/docview/3177467910/abstract/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch 
856 4 0 |3 Full Text  |u https://www.proquest.com/docview/3177467910/fulltext/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch 
856 4 0 |3 Full Text - PDF  |u https://www.proquest.com/docview/3177467910/fulltextPDF/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch