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Influence of High Eimeria tenella Immunization Dosages on Total Oocyst Output and Specific Antibodies Recognition Response in Hybrid Pullets (Gallus gallus)—A Pilot Study

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Gepubliceerd in:Antibodies vol. 14, no. 1 (2025), p. 9
Hoofdauteur: Juarez-Estrada, Marco A
Andere auteurs: Tellez-Isaias, Guillermo, Petrone-Garcia, Víctor M, Gayosso-Vazquez, Amanda, Hernandez-Velasco, Xochitl, Alonso-Morales, Rogelio A
Gepubliceerd in:
MDPI AG
Onderwerpen:
Clinical trials
Antibodies
Genetic modification
Antisera
Immunity
Immunization (passive)
Pattern recognition
Antigens
Dosage
Oocysts
Enzyme-linked immunosorbent assay
Immunization
Birds
Parasites
Protozoa
Polypeptides
Lymphocytes B
Poultry
Western blotting
Enzymes
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Samenvatting:Background: Two high primary-immunization doses of a wild-type E. tenella strain were assessed in healthy pullets (5K versus 10K sporulated oocysts/bird) to understand the effects of coccidia infection. Methods: Acquired immunity was evaluated following primary immunization and two booster doses with the homologous strain. Total oocyst shedding, clinical signs, and viability of every bird/group after each immunization/booster were recorded. Indirect ELISA measured the time course of humoral responses from each immunization group against sporozoite and second-generation merozoite of E. tenella. Antigen pattern recognition on these two asexual zoite stages of E. tenella was analyzed using Western blotting with antibodies from each immunization program. Afterwards, antigen recognition of specific life-cycle stages was performed using individual pullet serums from the best immunization program. Results: A primary-immunization dose of 1 × 104 oocysts/bird reduced the oocyst output; however, all pullets exhibited severe clinical signs and low specific antibodies titers, with decreased polypeptide recognition on both E. tenella asexual zoite stages. In contrast, immunization with 5 × 103 oocysts/bird yielded the best outcomes regarding increased oocyst collection and early development of sterilizing immunity. After the first booster dosage, this group’s antisera revealed a strong pattern of specific antigen recognition on the two assayed E. tenella life-cycle stages. Conclusions: The E. tenella-specific antibodies from the 5 × 103 oocysts/bird immunization program can aid in passive immunization trials and further research to identify B-cell immunoprotective antigens, which could help in the development of a genetically modified anticoccidial vaccine.
ISSN:2073-4468
DOI:10.3390/antib14010009
Bron:Biological Science Database