Obálka

First Detection and Molecular Characterization of Peach Latent Mosaic Viroid (PLMVd) in Kazakhstan

Uloženo v:
Vydáno v:Pathogens vol. 14, no. 3 (2025), p. 243
Hlavní autor: Stanbekova, Gulshan E
Další autoři: Nadirova, Leila T, Kryldakov, Ruslan V, Iskakov, Bulat K, Zhigailov, Andrey V
Vydáno:
MDPI AG
Témata:
United States > US
Kazakhstan
Trees
Phylogenetics
Cluster analysis
Nucleotides
Secondary structure
Gene sequencing
Propagation
Fruits
Cloning
DNA polymerase
Fruit trees
Invoices
Genomes
Surveys
Single-nucleotide polymorphism
Protein structure
Nucleotide sequence
Economic impact
Viroids
Climate change
On-line přístup:Citation/Abstract
Full Text + Graphics
Full Text - PDF
Tagy: Přidat tag
Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!
Abstrakt:Viroids represent obligate plant pathogens composed exclusively of non-protein coding small single-stranded RNAs that cause high economic losses worldwide. A field survey was carried out to assess the incidence of the peach latent mosaic viroid (PLMVd) in southeastern Kazakhstan, the region of the country where fruit trees are mainly grown. Of 246 stone fruit trees, 20 (8.13%) were infected with the PLMVd. The incidence of the PLMVd in the peach (19.23%; 15/78) was significantly higher than that in the apricot (6.76%; 5/74; p = 0.0234). Eight of the detected viroids were cloned and used for full-genome sequencing. The nucleotide sequence similarity of the selected isolates found in Kazakhstan was 83.9–100%. A phylogenetic analysis indicated three clusters for the Kazakhstani isolates of the PLMVd. Three groups of Kazakhstani viroids differed in their predicted secondary structure. During the survey, the PLMVd was detected and genetically characterized for the first time in Kazakhstan. The obtained results indicate the need to develop state control measures for the PLMVd, including regular monitoring surveys. We identified several SNPs of the PLMVd that had not been previously described. The results may be useful in optimizing diagnostic approaches for detecting stone fruit viroids and preventing their spread through propagation material.
ISSN:2076-0817
DOI:10.3390/pathogens14030243
Zdroj:Biological Science Database