Effect of Methionine supplementation on reproductive performance of Nili-Ravi buffaloes (Bubalus bubalis) treated with double Ovsycnch protocol of synchronization

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Publicado en:Pure and Applied Biology vol. 14, no. 1 (Mar 2025), p. 1
Autor principal: Waqas, Muhammad
Otros Autores: Hasan, Waseem, Asghar, Yasmeen, Tariq, Muhammad Sohaib, Munawar, Arslan, Hassan, Muhammad Muzzammil, Jamil, Huma
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International Society of Pure and Applied Biology
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022 |a 2304-2478 
024 7 |a 10.19045/bspab.2025.140001  |2 doi 
035 |a 3184104549 
045 2 |b d20250301  |b d20250331 
084 |a 185729  |2 nlm 
100 1 |a Waqas, Muhammad  |u Department of Theriogenology, Faculty of Veterinary Science, University of Agriculture Faisalabad, Pakistan 
245 1 |a Effect of Methionine supplementation on reproductive performance of Nili-Ravi buffaloes (Bubalus bubalis) treated with double Ovsycnch protocol of synchronization 
260 |b International Society of Pure and Applied Biology  |c Mar 2025 
513 |a Journal Article 
520 3 |a Estrus and conception are the two most important phases regarding the reproductive health of buffaloes. Estrus detection can increase conception rate, in this regard current research was designed to investigate the effect of methionine with double Ovsynch protocol on reproductive performance of Nili-Ravi buffaloes. The research was performed at Sar Buland Dairy Farm in Faisalabad, Punjab, Pakistan. A total of 40 animals were selected and divided into two groups labeled A and B. Group A was given methionine @25gram per animal per day up to the completion of double Ovsynch protocol of synchronization while Group B was subjected to synchronization protocol only. After completion of the protocol estrus intensity and preovulatory follicular size were monitored then timed artificial insemination (TAI) using frozen thawed semen was performed. Pregnancy rate and early embryonic mortality were checked through ultrasonography at Day 30 and 45 post-AI. Blood samples were taken at day 0 and day of AI then day 30 post-AI to determine serum progesterone and estradiol concentrations. Pregnancy rate, early embryonic death rate, serum estradiol at day 0, and serum progesterone at day 0 and 30 showed statistically non-significant differences between treatment and control groups (P>0.05) while pre-ovulatory follicular size, estrus intensity, and serum estradiol at the day of AI showed a statistically significant difference between treatment and control groups (P<0.05). Concludingly, research illustrates methionine in combination with double Ovsynch protocol improves estrus expression of Nili-Ravi buffaloes. 
653 |a Reproductive health 
653 |a Reproduction 
653 |a Pregnancy 
653 |a Investigations 
653 |a Estrus 
653 |a Dairy farms 
653 |a Animals 
653 |a Synchronization 
653 |a Synchronism 
653 |a Reproductive technologies 
653 |a Statistical analysis 
653 |a Semen 
653 |a Proteins 
653 |a Embryos 
653 |a Buffalo 
653 |a Methionine 
653 |a Progesterone 
653 |a 17β-Estradiol 
653 |a Fertility 
653 |a Artificial insemination 
653 |a Ultrasonic imaging 
653 |a Sex hormones 
653 |a Environmental 
700 1 |a Hasan, Waseem  |u Department of Clinical Medicine and Surgery, Faculty of Veterinary Science, University of Agriculture Faisalabad, Pakistan 
700 1 |a Asghar, Yasmeen  |u Department of Zoology, Ghazi University Dera Ghazi Khan, Pakistan 
700 1 |a Tariq, Muhammad Sohaib  |u Department of Theriogenology, Faculty of Veterinary Science, University of Agriculture Faisalabad, Pakistan 
700 1 |a Munawar, Arslan  |u Department of Theriogenology, Faculty of Veterinary Science, University of Agriculture Faisalabad, Pakistan 
700 1 |a Hassan, Muhammad Muzzammil 
700 1 |a Jamil, Huma 
773 0 |t Pure and Applied Biology  |g vol. 14, no. 1 (Mar 2025), p. 1 
786 0 |d ProQuest  |t Biological Science Database 
856 4 1 |3 Citation/Abstract  |u https://www.proquest.com/docview/3184104549/abstract/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch 
856 4 0 |3 Full Text  |u https://www.proquest.com/docview/3184104549/fulltext/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch 
856 4 0 |3 Full Text - PDF  |u https://www.proquest.com/docview/3184104549/fulltextPDF/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch