Mitochondria-targeted ROS scavenger JP4-039 improves cardiac function in a post-myocardial infarction animal model and induces angiogenesis in vitro
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| izdano v: | PLoS One vol. 20, no. 4 (Apr 2025), p. e0320703 |
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| Drugi avtorji: | , , , , , , , |
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Public Library of Science
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| Online dostop: | Citation/Abstract Full Text Full Text - PDF |
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|---|---|---|---|
| 001 | 3194483879 | ||
| 003 | UK-CbPIL | ||
| 022 | |a 1932-6203 | ||
| 024 | 7 | |a 10.1371/journal.pone.0320703 |2 doi | |
| 035 | |a 3194483879 | ||
| 045 | 2 | |b d20250401 |b d20250430 | |
| 084 | |a 174835 |2 nlm | ||
| 100 | 1 | |a Teixeira, Rayane Brinck | |
| 245 | 1 | |a Mitochondria-targeted ROS scavenger JP4-039 improves cardiac function in a post-myocardial infarction animal model and induces angiogenesis <i><b>in vitro</b></i> | |
| 260 | |b Public Library of Science |c Apr 2025 | ||
| 513 | |a Journal Article | ||
| 520 | 3 | |a BackgroundThis study aimed at evaluating the effects of JP4-039, a mitochondria-specific reactive oxygen species (mito-ROS) scavenger, on coronary angiogenesis and cardiac function in a post-myocardial infarction (MI) animal model.MethodsMice underwent ligation of the left anterior descending (LAD) artery to induce MI and received intraperitoneal (i.p.) injections of JP4-039 or vehicle (n=8 animals/group) three times/week for four weeks. Echocardiography for cardiac function and immunohistochemistry for Infarction area and capillary density were carried out. Angiogenic potential of endothelial cells (EC) was assessed by ex vivo tube formation using mouse heart EC (MHEC) and by aortic and atrial sprouting. Western blots were conducted using mouse cardiac tissue and lysates from HCAECs that were treated with or without JP4-039.ResultsCardiac function including ejection fraction, fractional shortening, and fractional area change were improved significantly in JP4-039-treated animals compared to the vehicle group. JP4-039-treated hearts demonstrated significant reduction in infarction size and increased capillary density in the ischemic area. These findings were consistent with increased ex vivo endothelial sprouting of the aortae and atrial tissue from the mice treated with JP4-039. Western blots using cardiac tissue lysates from JP4-039-treated animals showed decrease in phosphorylation of AMPKα at the Threonine 172, suggesting a plausible increase in the ATP:AMP ratio. Interestingly, JP4-039 increased expression of mitochondrial complexes I and IV and increased ATP synthesis in EC.ConclusionsJP4-039-mediated reduction in mito-ROS results in significantly increased coronary vascular density in ischemic myocardium, improved ATP synthesis, and recovery of post-MI cardiac function. Together, these results suggest that nitroxide nanodrug-mediated reduction in mito-ROS may help recover post-MI cardiac function. | |
| 610 | 4 | |a Nikon Instruments Inc | |
| 651 | 4 | |a United States--US | |
| 651 | 4 | |a Germany | |
| 653 | |a Mitochondria | ||
| 653 | |a Aorta | ||
| 653 | |a Heart | ||
| 653 | |a Phosphorylation | ||
| 653 | |a Cytochrome | ||
| 653 | |a Nitroxide | ||
| 653 | |a Heart failure | ||
| 653 | |a Ischemia | ||
| 653 | |a Hypoxia | ||
| 653 | |a Reactive oxygen species | ||
| 653 | |a Antioxidants | ||
| 653 | |a Myocardial infarction | ||
| 653 | |a Lysates | ||
| 653 | |a Animal models | ||
| 653 | |a Synthesis | ||
| 653 | |a Density | ||
| 653 | |a Echocardiography | ||
| 653 | |a Myocardium | ||
| 653 | |a Angiogenesis | ||
| 653 | |a Homeostasis | ||
| 653 | |a Animals | ||
| 653 | |a Heart attacks | ||
| 653 | |a Endothelial cells | ||
| 653 | |a Coronary vessels | ||
| 653 | |a Cardiovascular disease | ||
| 653 | |a Immunohistochemistry | ||
| 653 | |a Veins & arteries | ||
| 653 | |a Cardiac function | ||
| 653 | |a Western blotting | ||
| 653 | |a Social | ||
| 700 | 1 | |a Albro, Jane H | |
| 700 | 1 | |a Sabra, Mohamed | |
| 700 | 1 | |a Abedin, Taslova | |
| 700 | 1 | |a Tucker, Aja N | |
| 700 | 1 | |a Raj Sidharth | |
| 700 | 1 | |a Sellke, Frank W | |
| 700 | 1 | |a Wipf, Peter | |
| 700 | 1 | |a Abid, M Ruhul | |
| 773 | 0 | |t PLoS One |g vol. 20, no. 4 (Apr 2025), p. e0320703 | |
| 786 | 0 | |d ProQuest |t Health & Medical Collection | |
| 856 | 4 | 1 | |3 Citation/Abstract |u https://www.proquest.com/docview/3194483879/abstract/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text |u https://www.proquest.com/docview/3194483879/fulltext/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text - PDF |u https://www.proquest.com/docview/3194483879/fulltextPDF/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |