Microscopic and Molecular Identification of Eimeria Species in Domestic Rabbits (Oryctolagus cuniculus) in Romania
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| Argitaratua izan da: | Animals vol. 15, no. 8 (2025), p. 1109 |
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MDPI AG
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| Sarrera elektronikoa: | Citation/Abstract Full Text + Graphics Full Text - PDF |
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| 024 | 7 | |a 10.3390/ani15081109 |2 doi | |
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| 045 | 2 | |b d20250101 |b d20251231 | |
| 084 | |a 231334 |2 nlm | ||
| 100 | 1 | |a Jitea (Sîrbu) Beatrice Ana-Maria |u Faculty of Veterinary Medicine, University of Life Sciences “King Michael I” from Timisoara, Calea Aradului 119, 300645 Timisoara, Romania; sorinmorariu@usvt.ro (S.M.); mirela.imre@usvt.ro (M.I.); tijana.florea@usvt.ro (T.F.); iasmina.luca@usvt.ro (I.L.); gheorghedarabus@usvt.ro (G.D.) | |
| 245 | 1 | |a Microscopic and Molecular Identification of <i>Eimeria</i> Species in Domestic Rabbits (<i>Oryctolagus cuniculus</i>) in Romania | |
| 260 | |b MDPI AG |c 2025 | ||
| 513 | |a Journal Article | ||
| 520 | 3 | |a Coccidiosis caused by Eimeria spp. is a parasitic disease with high morbidity that affects various animal species, including rabbits. In rabbits, eleven Eimeria species have been primarily identified through microscopic examination of oocysts; however, this technique has certain limitations that make it challenging to differentiate between Eimeria species. The main objective of this study was to morphologically identify Eimeria species infecting rabbits in Romania, as well as to confirm these identifications using molecular biology techniques, specifically through the ITS-1 rRNA gene. A total of 236 samples were collected from different regions of Romania, of which 77.56% (183/236) tested positive for at least one Eimeria species. The samples were collected from rabbits raised in individual household settings, under various management systems—both in individual cages and in common ground-based enclosures. The areas of origin were chosen randomly, without applying restrictive criteria, specifically to ensure broad representativeness of the farming conditions encountered in practice, regardless of the production system or geographic specificity. The microscopic examination of morphological characteristics identified ten Eimeria species, whereas PCR results confirmed the presence of four species. It was not always possible to identify all the species present in a given sample, likely due to the predominant parasitic load and the DNA quantity of each species. The results of this study confirm that ITS-1 is a useful molecular marker for identifying Eimeria species in domestic rabbits. | |
| 651 | 4 | |a United States--US | |
| 651 | 4 | |a Romania | |
| 653 | |a Sediments | ||
| 653 | |a Morbidity | ||
| 653 | |a Protozoa | ||
| 653 | |a Rabbits | ||
| 653 | |a Feces | ||
| 653 | |a Parasitic diseases | ||
| 653 | |a Parasites | ||
| 653 | |a Identification | ||
| 653 | |a Mortality | ||
| 653 | |a Morphology | ||
| 653 | |a Statistical analysis | ||
| 700 | 1 | |a Morariu Sorin |u Faculty of Veterinary Medicine, University of Life Sciences “King Michael I” from Timisoara, Calea Aradului 119, 300645 Timisoara, Romania; sorinmorariu@usvt.ro (S.M.); mirela.imre@usvt.ro (M.I.); tijana.florea@usvt.ro (T.F.); iasmina.luca@usvt.ro (I.L.); gheorghedarabus@usvt.ro (G.D.) | |
| 700 | 1 | |a Imre Mirela |u Faculty of Veterinary Medicine, University of Life Sciences “King Michael I” from Timisoara, Calea Aradului 119, 300645 Timisoara, Romania; sorinmorariu@usvt.ro (S.M.); mirela.imre@usvt.ro (M.I.); tijana.florea@usvt.ro (T.F.); iasmina.luca@usvt.ro (I.L.); gheorghedarabus@usvt.ro (G.D.) | |
| 700 | 1 | |a Florea Tiana |u Faculty of Veterinary Medicine, University of Life Sciences “King Michael I” from Timisoara, Calea Aradului 119, 300645 Timisoara, Romania; sorinmorariu@usvt.ro (S.M.); mirela.imre@usvt.ro (M.I.); tijana.florea@usvt.ro (T.F.); iasmina.luca@usvt.ro (I.L.); gheorghedarabus@usvt.ro (G.D.) | |
| 700 | 1 | |a Bogdan, Sîrbu Cătălin |u Faculty of Bioengineering of Animal Resources, University of Life Sciences “King Michael I” from Timisoara, Calea Aradului 119, 300645 Timisoara, Romania | |
| 700 | 1 | |a Iasmina, Luca |u Faculty of Veterinary Medicine, University of Life Sciences “King Michael I” from Timisoara, Calea Aradului 119, 300645 Timisoara, Romania; sorinmorariu@usvt.ro (S.M.); mirela.imre@usvt.ro (M.I.); tijana.florea@usvt.ro (T.F.); iasmina.luca@usvt.ro (I.L.); gheorghedarabus@usvt.ro (G.D.) | |
| 700 | 1 | |a Dumitru Simona |u Veterinary and Food Safety Department, 4 Surorile Martir Caceu, 300585 Timisoara, Romania; simonagiubega@gmail.com | |
| 700 | 1 | |a Dărăbuș Gheorghe |u Faculty of Veterinary Medicine, University of Life Sciences “King Michael I” from Timisoara, Calea Aradului 119, 300645 Timisoara, Romania; sorinmorariu@usvt.ro (S.M.); mirela.imre@usvt.ro (M.I.); tijana.florea@usvt.ro (T.F.); iasmina.luca@usvt.ro (I.L.); gheorghedarabus@usvt.ro (G.D.) | |
| 773 | 0 | |t Animals |g vol. 15, no. 8 (2025), p. 1109 | |
| 786 | 0 | |d ProQuest |t Publicly Available Content Database | |
| 856 | 4 | 1 | |3 Citation/Abstract |u https://www.proquest.com/docview/3194486196/abstract/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text + Graphics |u https://www.proquest.com/docview/3194486196/fulltextwithgraphics/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text - PDF |u https://www.proquest.com/docview/3194486196/fulltextPDF/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |