A synonymous KCNH2 polymorphism and methadone trough level influence QTc prolongation in Kelantanese Malay recipients of methadone maintenance therapy (MMT) in Malaysia

-д хадгалсан:
Номзүйн дэлгэрэнгүй
-д хэвлэсэн:PLoS One vol. 20, no. 5 (May 2025), p. e0322724
Үндсэн зохиолч: Muhammad Irfan Abdul Jalal
Бусад зохиолчид: Abdullah-Zawawi, Muhammad-Redha, Nurfadhlina Musa, Ghazali, Basyirah, Zahari, Zalina, Nasir Mohamad
Хэвлэсэн:
Public Library of Science
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Онлайн хандалт:Citation/Abstract
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LEADER 00000nab a2200000uu 4500
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022 |a 1932-6203 
024 7 |a 10.1371/journal.pone.0322724  |2 doi 
035 |a 3200695396 
045 2 |b d20250501  |b d20250531 
084 |a 174835  |2 nlm 
100 1 |a Muhammad Irfan Abdul Jalal 
245 1 |a A synonymous KCNH2 polymorphism and methadone trough level influence QTc prolongation in Kelantanese Malay recipients of methadone maintenance therapy (MMT) in Malaysia 
260 |b Public Library of Science  |c May 2025 
513 |a Journal Article 
520 3 |a Potassium voltage-gated channel subfamily H member 2 (KCNH2) polymorphisms have been found to influence the heart-rate adjusted QT (QTc) intervals. We investigated the association between KCNH2 polymorphisms and QTc intervals among Malay opioid-dependent methadone maintenance treatment (MMT) recipients. A cross-sectional study was conducted involving 111 patients with stable methadone dosage for at least 6 months attending several methadone clinics in Kelantan, Malaysia between March 2011 and October 2012. Those with cardiac structural defects, recipients of other QTc-prolonging pharmacotherapeutic agents, had aggressive behavior or other active psychiatric illnesses, chronic medical and surgical ailments and who were unable to communicate in Malay and English were excluded. The Fridericia-corrected QTc intervals were recorded using a 12-lead electrocardiogram. DNA samples were extracted from peripheral blood leukocytes and genotyped using nested allele-specific polymerase chain reaction for these four KCNH2 polymorphisms: 1539C > T, 1956T > C, 2350C > T, and 2690A > C. Mean QTc interval is 408 ms (SD: 24). Molecular docking was performed on all four KCNH2 polymorphisms to investigate the impact of the nucleotide changes on methadone binding. Based on multiple regression analysis, only 1539T > C polymorphism (βadjusted: 10.506 (95% CI:0.846, 20.166), p = 0.033; recessive model), serum methadone trough (βadjusted: 0.025 (95% CI: 0.006, 0.043), p = 0.009), potassium (βadjusted: -8.756 (95% CI: -15.938, -1.575), p = 0.017) and magnesium (βadjusted: -106.226 (95% CI: -159.291, -53.161), p < 0.001) levels were significantly associated with mean QTc. Molecular docking analysis resulted in good binding-energy values between the 1539C > T and methadone, with the formation of hydrophobic and π–π stacking interactions, suggesting that 1539C > T was the newly discovered SNP involved in QTc prolongation. In conclusion, the 1539C > T KCNH2 polymorphism is associated with QTc prolongation in our MMT recipients, necessitating QTc monitoring to prevent methadone-associated cardiotoxicity in this Malay MMT population. 
610 4 |a Universiti Sains Malaysia 
651 4 |a Malaysia 
651 4 |a United States--US 
653 |a Magnesium 
653 |a Drug withdrawal 
653 |a Energy value 
653 |a Electrocardiography 
653 |a Maintenance 
653 |a Binding 
653 |a Heart rate 
653 |a Regression analysis 
653 |a EKG 
653 |a Leukocytes 
653 |a Potassium 
653 |a Ethics 
653 |a Intervals 
653 |a Aggressive behavior 
653 |a Drug dosages 
653 |a Narcotics 
653 |a Multiple regression analysis 
653 |a Polymorphism 
653 |a Sample size 
653 |a Patient safety 
653 |a Human immunodeficiency virus--HIV 
653 |a Potassium channels (voltage-gated) 
653 |a Nucleotides 
653 |a Polymerase chain reaction 
653 |a Molecular docking 
653 |a Methadone 
653 |a Genetic engineering 
653 |a Single-nucleotide polymorphism 
653 |a Channel gating 
653 |a Peripheral blood 
653 |a Cardiotoxicity 
653 |a Methamphetamine 
653 |a Prolongation 
653 |a Hydrophobicity 
653 |a Social 
700 1 |a Abdullah-Zawawi, Muhammad-Redha 
700 1 |a Nurfadhlina Musa 
700 1 |a Ghazali, Basyirah 
700 1 |a Zahari, Zalina 
700 1 |a Nasir Mohamad 
773 0 |t PLoS One  |g vol. 20, no. 5 (May 2025), p. e0322724 
786 0 |d ProQuest  |t Health & Medical Collection 
856 4 1 |3 Citation/Abstract  |u https://www.proquest.com/docview/3200695396/abstract/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch 
856 4 0 |3 Full Text  |u https://www.proquest.com/docview/3200695396/fulltext/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch 
856 4 0 |3 Full Text - PDF  |u https://www.proquest.com/docview/3200695396/fulltextPDF/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch