Effects of Bacteriophage on Antibacterial Properties, Nonspecific Immune Responses, and Gut Microbiota in Litopenaeus vannamei Post Vibrio parahaemolyticus Infection
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| Publicado en: | Aquaculture Research vol. 2025 (2025) |
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John Wiley & Sons, Inc.
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| Acceso en línea: | Citation/Abstract Full Text Full Text - PDF |
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| 001 | 3202632660 | ||
| 003 | UK-CbPIL | ||
| 022 | |a 1355-557X | ||
| 022 | |a 1365-2109 | ||
| 022 | |a 0266-996X | ||
| 022 | |a 0141-9862 | ||
| 024 | 7 | |a 10.1155/are/7731145 |2 doi | |
| 035 | |a 3202632660 | ||
| 045 | 2 | |b d20250101 |b d20251231 | |
| 084 | |a 165932 |2 nlm | ||
| 100 | 1 | |a Song, Dongdong |u Key Laboratory of Microecological Resources and Utilization in Breeding Industry Ministry of Agriculture and Rural Affairs Guangzhou 511400 China; Key Laboratory of Tropical Marine Bio-resources and Ecology South China Sea Institute of Oceanology Chinese Academy of Sciences Guangzhou 510301 China; Guangdong Haid Group Co. Ltd. Guangzhou 511400 China | |
| 245 | 1 | |a Effects of Bacteriophage on Antibacterial Properties, Nonspecific Immune Responses, and Gut Microbiota in <i>Litopenaeus vannamei</i> Post <i>Vibrio parahaemolyticus</i> Infection | |
| 260 | |b John Wiley & Sons, Inc. |c 2025 | ||
| 513 | |a Journal Article | ||
| 520 | 3 | |a Effective immune regulation and balanced gut microbiota play important roles in preventing pathogen infections in Litopenaeus vannamei farming. Bacteriophages are a promising candidate in pathogen control for their specific antibacterial properties. While previous studies focused on the direct antibacterial effects of phages, their effects on nonspecific immune responses and gut microbiota after infection remains to be less explored. In this study, a lytic Vibrio parahaemolyticus phage was isolated from wastewater with a broad host range (66.7% lytic efficiency), low multiplicity of infection (MOI; 0.1), and high environmental tolerance (pH: 3–11; temperature: 4–60°C). Whole genome analysis revealed a 93,814 bp double-stranded linear DNA molecule with 45.1% GC. Both the in vitro cocultivation (24 h) and in vivo shrimp cultivation trails (7 days) demonstrated that phage could effectively reduce the quantities of Vibrio (>99%). The in vivo phage fed shrimp exhibited elevated levels of nonspecific immune-related enzymes like alkaline phosphatase (AKP), catalase (CAT), superoxide dismutase (SOD), phenoloxidase (PO), and lysozyme (LZM) and upregulated immune-related gene expression including those of antimicrobial peptides, pattern recognition receptors (PRRs), and pattern recognition proteins. Additionally, phage treatment improved the diversity of the gut microbiota (Shannon-10 index) after Vibrio infection, indicating restored microbial balance in shrimp. These results suggest that phage therapy promotes nonspecific immune responses and repair intestinal dysbacteriosis in shrimp after Vibrio infection, elucidating a promising strategy to treat pathogenic Vibrio in shrimp aquaculture. | |
| 651 | 4 | |a China | |
| 653 | |a Phages | ||
| 653 | |a Superoxide dismutase | ||
| 653 | |a Immunoregulation | ||
| 653 | |a Wastewater | ||
| 653 | |a Gastrointestinal tract | ||
| 653 | |a Lysozyme | ||
| 653 | |a Shrimps | ||
| 653 | |a Virulence | ||
| 653 | |a Pattern recognition | ||
| 653 | |a Bacteria | ||
| 653 | |a Genomes | ||
| 653 | |a Immune response | ||
| 653 | |a Shrimp culture | ||
| 653 | |a Microorganisms | ||
| 653 | |a Gene expression | ||
| 653 | |a Antibiotics | ||
| 653 | |a Dysbacteriosis | ||
| 653 | |a Temperature tolerance | ||
| 653 | |a Microbiota | ||
| 653 | |a Alkaline phosphatase | ||
| 653 | |a Genomic analysis | ||
| 653 | |a Peptides | ||
| 653 | |a Aquaculture | ||
| 653 | |a Digestive system | ||
| 653 | |a Enzymes | ||
| 653 | |a Phosphatase | ||
| 653 | |a Pathogens | ||
| 653 | |a Infections | ||
| 653 | |a Multiplicity of infection | ||
| 653 | |a Antibacterial activity | ||
| 653 | |a Host range | ||
| 653 | |a Antioxidants | ||
| 653 | |a Microbial flora | ||
| 653 | |a Intestinal microflora | ||
| 653 | |a Catalase | ||
| 653 | |a Bacteriophages | ||
| 653 | |a Immunity (Disease) | ||
| 653 | |a Phenoloxidase | ||
| 653 | |a Marine crustaceans | ||
| 653 | |a Immune system | ||
| 653 | |a Pattern recognition receptors | ||
| 653 | |a Immunological tolerance | ||
| 653 | |a Antimicrobial peptides | ||
| 653 | |a Toxins | ||
| 653 | |a Litopenaeus vannamei | ||
| 653 | |a Vibrio parahaemolyticus | ||
| 653 | |a Environmental | ||
| 653 | |a Vibrio | ||
| 700 | 1 | |a Shi, Baojun |u Key Laboratory of Microecological Resources and Utilization in Breeding Industry Ministry of Agriculture and Rural Affairs Guangzhou 511400 China; Guangdong Haid Group Co. Ltd. Guangzhou 511400 China | |
| 700 | 1 | |a Huang, Jinlu |u Guangdong Haid Group Co. Ltd. Guangzhou 511400 China; Qingyuan Haibei BIO-TECH Co. Ltd. Qingyuan 511853 China | |
| 700 | 1 | |a Wang, Jian |u Key Laboratory of Microecological Resources and Utilization in Breeding Industry Ministry of Agriculture and Rural Affairs Guangzhou 511400 China; Guangdong Haid Group Co. Ltd. Guangzhou 511400 China | |
| 773 | 0 | |t Aquaculture Research |g vol. 2025 (2025) | |
| 786 | 0 | |d ProQuest |t Publicly Available Content Database | |
| 856 | 4 | 1 | |3 Citation/Abstract |u https://www.proquest.com/docview/3202632660/abstract/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text |u https://www.proquest.com/docview/3202632660/fulltext/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text - PDF |u https://www.proquest.com/docview/3202632660/fulltextPDF/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |