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Physiological and Transcriptome Analyses of Gill and Hepatopancreas of Potamocorbula ustulata Under Ammonia Exposure

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Publicado en:Fishes vol. 10, no. 5 (2025), p. 200
Autor principal: He, Jing
Otros Autores: Wang, Xinhui, Wu, Mingyu, Lin, Zhihua, He, Lin, Zheng Xiafei
Publicado:
MDPI AG
Materias:
United States > US
Shanghai China
China
Mollusks
Physiology
Shellfish
Glutamate-ammonia ligase
Enzymatic activity
Amino acids
Population density
Ammonia
Metabolism
MAP kinase
Gills
Oxidative phosphorylation
Hepatopancreas
Excretion
H+-transporting ATPase
Urea
Stress response
Nitrogen
Antioxidants
Gene expression
Apoptosis
Experiments
Seawater
Glutamate dehydrogenase
Glutamine
Burrowing behavior
Transcriptome
Enzymes
Ammonium transporter
Transcriptomes
Na+/K+-exchanging ATPase
Genes
Software
Toxicity
Phosphorylation
Ammonium
Molecular modelling
NF-κB protein
Inflammation
Ammonium compounds
Signal transduction
Enzyme activity
Potamocorbula
Acceso en línea:Citation/Abstract
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Resumen:Excessive ammonia accumulation poses a significant threat to aquatic species. Potamocorbula ustulata, known for its burrowing behavior and high population density, may experience elevated ammonia levels in its environment. However, its ammonia stress response mechanisms remain unclear. This study investigates the physiological and molecular responses of P. ustulata to acute ammonia exposure. Antioxidant enzyme activity was significantly altered in the gills and hepatopancreas, with GS, GDH, and ARG levels markedly increasing in the hepatopancreas. Transcriptome analysis revealed that after 24 h of exposure, differentially expressed genes (DEGs) were enriched in apoptosis and inflammation-related pathways (MAPK, NF-kB, NOD-like receptor signaling). By 96 h, DEGs in the gills were associated with nitrogen metabolism and transport, while those in the hepatopancreas were linked to oxidative phosphorylation and amino acid metabolism. Key ammonia transport and excretion genes, including V-type H+-ATPase, Ammonium transporter Rh, and Na+/K+-ATPase, were significantly upregulated in the gills, while glutamine synthetase and glutamate dehydrogenase were upregulated in the hepatopancreas (p < 0.05). These findings suggest that ammonia stress disrupts antioxidant defense, triggers inflammation and apoptosis, and enhances ammonia tolerance through excretion, glutamine conversion, and urea synthesis. This study provides insights into the molecular mechanisms underlying ammonia tolerance in bivalves.
ISSN:2410-3888
DOI:10.3390/fishes10050200
Fuente:Biological Science Database