Horizontal cell connectivity in the anchovy retina—a 3D electron microscopic study

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Foilsithe in:BMC Biology vol. 23 (2025), p. 1
Príomhchruthaitheoir: Guder, Petra
Rannpháirtithe: Scheungrab, Max, Kohnert, Peter, Kolyfetis, Georgios, Wanner, Gerhard, Heß, Martin
Foilsithe / Cruthaithe:
Springer Nature B.V.
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Rochtain ar líne:Citation/Abstract
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100 1 |a Guder, Petra 
245 1 |a Horizontal cell connectivity in the anchovy retina—a 3D electron microscopic study 
260 |b Springer Nature B.V.  |c 2025 
513 |a Journal Article 
520 3 |a BackgroundBlock-face scanning electron microscopy has opened a new era of connectomics research, in which it is possible to make dense reconstructions of all cells in a clipping of a neuronal network, such as the retina, resolving synaptic contacts. Anchovies, exceptionally abundant marine teleosts, have retinae with regions for triple cone-based color vision and a region with specialized cone photoreceptors, so-called polycones, made of long and short cones with axially oriented outer segment lamellae for polarization contrast vision. This modality, discovered in the 1970s, is unique in vertebrates, but the neural wiring for contrast generation in deeper retinal layers is unknown so far.ResultsTo elucidate the retinal connectomics of the European anchovy Engraulis encrasicolus (Linnaeus, 1758), in a first project, we investigated the shapes and cone-specific wiring rules of 3 horizontal cell types using volume electron microscopy and subsequent computer-aided reconstruction: H1 cells contact both cone types of the polycone, H2 cells contact only the short cones, and H3 cells are exclusively connected to rods. In addition, a distinctive double band of Müller fibers and a layer of H1 axon terminals were structurally clarified.ConclusionsThe findings suggest that (1) the monochromatic polarization contrast system based on fine structure specializations in the outer retina is connected to an inherited (bichromatic) color contrast mechanism in the inner retina, (2) the anchovy polycones arose from red (now long) and green (now short) cones, and (3) the blue single cones disappeared in the relevant retinal region. 
653 |a Color vision 
653 |a Fine structure 
653 |a Scanning electron microscopy 
653 |a Photoreceptors 
653 |a Retina 
653 |a Computer terminals 
653 |a Lamellae 
653 |a Vertebrates 
653 |a Presynapse 
653 |a Anchovies 
653 |a Polarization 
653 |a Horizontal cells 
653 |a Neurons 
653 |a Cones 
653 |a Electron microscopy 
653 |a Image reconstruction 
653 |a Neural networks 
653 |a Ultrastructure 
653 |a Wiring 
653 |a Light 
653 |a Rod outer segment membranes 
653 |a Engraulis encrasicolus 
700 1 |a Scheungrab, Max 
700 1 |a Kohnert, Peter 
700 1 |a Kolyfetis, Georgios 
700 1 |a Wanner, Gerhard 
700 1 |a Heß, Martin 
773 0 |t BMC Biology  |g vol. 23 (2025), p. 1 
786 0 |d ProQuest  |t Health & Medical Collection 
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