Mapping the molecular signature of ABA-regulated gene expression in germinating barley embryos
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| Udgivet i: | BMC Plant Biology vol. 25 (2025), p. 1 |
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| Andre forfattere: | , , , , , , |
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Springer Nature B.V.
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| LEADER | 00000nab a2200000uu 4500 | ||
|---|---|---|---|
| 001 | 3216560238 | ||
| 003 | UK-CbPIL | ||
| 022 | |a 1471-2229 | ||
| 024 | 7 | |a 10.1186/s12870-025-06654-z |2 doi | |
| 035 | |a 3216560238 | ||
| 045 | 2 | |b d20250101 |b d20251231 | |
| 084 | |a 58484 |2 nlm | ||
| 100 | 1 | |a Ewa Sybilskahareh Sadat Haddadi | |
| 245 | 1 | |a Mapping the molecular signature of ABA-regulated gene expression in germinating barley embryos | |
| 260 | |b Springer Nature B.V. |c 2025 | ||
| 513 | |a Journal Article | ||
| 520 | 3 | |a BackgroundAbscisic acid (ABA) regulates key plant processes, including seed germination, dormancy, and abiotic stress responses. While its physiological role in germination is well-documented, the molecular mechanisms are still poorly understood. To address this, we analyzed transcriptomic and metabolomic changes in ABA-treated germinating barley (Hordeum vulgare) embryos. To map ABA-responsive gene expression across embryonic tissues, we employed the Visium Spatial Transcriptomics (10× Genomics). This approach, which remains technically challenging to be applied in plant tissues, enabled the precise localization of gene expression across six embryo regions, offering insights into tissue-specific expression patterns that cannot be resolved by traditional RNA-seq.ResultsTranscriptomic analysis indicated that ABA acts primarily as a germination repressor. Gene ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses linked ABA-inhibited genes to energy metabolism, lignin biosynthesis, cell wall organization, and photosynthesis, while induced genes were associated with environmental adaptation and phytohormone signaling. Differentially expressed genes (DEGs) correlated with metabolites involved in phytohormone pathways, including gibberellins, jasmonates, brassinosteroids, salicylic acid, auxins, and ABA metabolism. Comparisons with developing seed transcriptomes suggested an ABA-associated gene expression signature in embryos. Spatial transcriptomics technique made possible the precise identification of ABA-induced transcriptional changes within distinct embryonic tissues.ConclusionsIntegrating transcriptomics, metabolomics and spatial transcriptomics defined the molecular signature of ABA-induced modulation of phytohormonal crosstalk, energy metabolism, and tissue-specific gene activity in germinating seeds. The successful use of spatial transcriptomics adds a novel layer of resolution for understanding tissue-specific ABA responses during barley seed germination. These findings offer new insights into the ABA role in seed germination and potential strategies for enhancing crop resilience. | |
| 651 | 4 | |a Poland | |
| 651 | 4 | |a United States--US | |
| 653 | |a Energy metabolism | ||
| 653 | |a Biosynthesis | ||
| 653 | |a Binding sites | ||
| 653 | |a Barley | ||
| 653 | |a Metabolites | ||
| 653 | |a Metabolism | ||
| 653 | |a Phytohormones | ||
| 653 | |a Embryos | ||
| 653 | |a Genomics | ||
| 653 | |a Kinases | ||
| 653 | |a Stress response | ||
| 653 | |a Crosstalk | ||
| 653 | |a Seed germination | ||
| 653 | |a Gibberellins | ||
| 653 | |a Gene expression | ||
| 653 | |a Crop resilience | ||
| 653 | |a Salicylic acid | ||
| 653 | |a Brassinosteroids | ||
| 653 | |a Seeds | ||
| 653 | |a Transcription factors | ||
| 653 | |a Transcriptomes | ||
| 653 | |a Jasmonic acid | ||
| 653 | |a Plant tissues | ||
| 653 | |a Cell walls | ||
| 653 | |a Genes | ||
| 653 | |a Metabolomics | ||
| 653 | |a Abscisic acid | ||
| 653 | |a Gene mapping | ||
| 653 | |a Molecular modelling | ||
| 653 | |a Germination | ||
| 653 | |a Transcriptomics | ||
| 653 | |a Photosynthesis | ||
| 653 | |a Dormancy | ||
| 653 | |a Localization | ||
| 653 | |a Plant hormones | ||
| 653 | |a Auxins | ||
| 653 | |a Environmental | ||
| 700 | 1 | |a Mur, Luis A J | |
| 700 | 1 | |a Beckmann, Manfred | |
| 700 | 1 | |a Hryhorowicz, Szymon | |
| 700 | 1 | |a Suszynska-Zajczyk, Joanna | |
| 700 | 1 | |a Knaur, Monika | |
| 700 | 1 | |a Pławski, Andrzej | |
| 700 | 1 | |a Daszkowska-Golec, Agata | |
| 773 | 0 | |t BMC Plant Biology |g vol. 25 (2025), p. 1 | |
| 786 | 0 | |d ProQuest |t Health & Medical Collection | |
| 856 | 4 | 1 | |3 Citation/Abstract |u https://www.proquest.com/docview/3216560238/abstract/embedded/L8HZQI7Z43R0LA5T?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text |u https://www.proquest.com/docview/3216560238/fulltext/embedded/L8HZQI7Z43R0LA5T?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text - PDF |u https://www.proquest.com/docview/3216560238/fulltextPDF/embedded/L8HZQI7Z43R0LA5T?source=fedsrch |