Advances in spatial transcriptomics and its application in the musculoskeletal system

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Publicat a:Bone Research vol. 13, no. 1 (2025), p. 54
Autor principal: Wang, Haoyu
Altres autors: Cheng, Peng, Wang, Juan, Lv, Hongzhi, Han, Jie, Hou, Zhiyong, Xu, Ren, Chen, Wei
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Springer Nature B.V.
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100 1 |a Wang, Haoyu  |u Hebei Medical University Third Hospital, Department of Orthopedic Surgery, Shijiazhuang, China (GRID:grid.256883.2) (ISNI:0000 0004 1760 8442); Key Laboratory of Biomechanics of Hebei Province, Shijiazhuang, China (GRID:grid.452209.8) (ISNI:0000 0004 1799 0194); NHC Key Laboratory of Intelligent Orthopedic Equipment, Shijiazhuang, China (GRID:grid.452209.8) 
245 1 |a Advances in spatial transcriptomics and its application in the musculoskeletal system 
260 |b Springer Nature B.V.  |c 2025 
513 |a Journal Article 
520 3 |a While bulk RNA sequencing and single-cell RNA sequencing have shed light on cellular heterogeneity and potential molecular mechanisms in the musculoskeletal system in both physiological and various pathological states, the spatial localization of cells and molecules and intercellular interactions within the tissue context require further elucidation. Spatial transcriptomics has revolutionized biological research by simultaneously capturing gene expression profiles and in situ spatial information of tissues, gradually finding applications in musculoskeletal research. This review provides a summary of recent advances in spatial transcriptomics and its application to the musculoskeletal system. The classification and characteristics of data acquisition techniques in spatial transcriptomics are briefly outlined, with an emphasis on widely-adopted representative technologies and the latest technological breakthroughs, accompanied by a concise workflow for incorporating spatial transcriptomics into musculoskeletal system research. The role of spatial transcriptomics in revealing physiological mechanisms of the musculoskeletal system, particularly during developmental processes, is thoroughly summarized. Furthermore, recent discoveries and achievements of this emerging omics tool in addressing inflammatory, traumatic, degenerative, and tumorous diseases of the musculoskeletal system are compiled. Finally, challenges and potential future directions for spatial transcriptomics, both as a field and in its applications in the musculoskeletal system, are discussed. 
653 |a Biomedical research 
653 |a Physiology 
653 |a Cells 
653 |a Gene expression 
653 |a Bone marrow 
653 |a Genomics 
653 |a Localization 
653 |a Signal to noise ratio 
653 |a Musculoskeletal system 
700 1 |a Cheng, Peng  |u Huazhong University of Science and Technology, Department of Orthopedics, Union Hospital, Tongji Medical College, Wuhan, China (GRID:grid.33199.31) (ISNI:0000 0004 0368 7223) 
700 1 |a Wang, Juan  |u Hebei Medical University Third Hospital, Department of Orthopedic Surgery, Shijiazhuang, China (GRID:grid.256883.2) (ISNI:0000 0004 1760 8442); Key Laboratory of Biomechanics of Hebei Province, Shijiazhuang, China (GRID:grid.452209.8) (ISNI:0000 0004 1799 0194); NHC Key Laboratory of Intelligent Orthopedic Equipment, Shijiazhuang, China (GRID:grid.452209.8) 
700 1 |a Lv, Hongzhi  |u Hebei Medical University Third Hospital, Department of Orthopedic Surgery, Shijiazhuang, China (GRID:grid.256883.2) (ISNI:0000 0004 1760 8442); Key Laboratory of Biomechanics of Hebei Province, Shijiazhuang, China (GRID:grid.452209.8) (ISNI:0000 0004 1799 0194); NHC Key Laboratory of Intelligent Orthopedic Equipment, Shijiazhuang, China (GRID:grid.452209.8) 
700 1 |a Han, Jie  |u Xiamen University, State Key Laboratory of Cellular Stress Biology, Cancer Research Center, School of Medicine, Faculty of Medicine and Life Sciences, Xiamen, China (GRID:grid.12955.3a) (ISNI:0000 0001 2264 7233) 
700 1 |a Hou, Zhiyong  |u Hebei Medical University Third Hospital, Department of Orthopedic Surgery, Shijiazhuang, China (GRID:grid.256883.2) (ISNI:0000 0004 1760 8442); Key Laboratory of Biomechanics of Hebei Province, Shijiazhuang, China (GRID:grid.452209.8) (ISNI:0000 0004 1799 0194); NHC Key Laboratory of Intelligent Orthopedic Equipment, Shijiazhuang, China (GRID:grid.452209.8) 
700 1 |a Xu, Ren  |u Xiamen University, The First Affiliated Hospital of Xiamen University-ICMRS Collaborating Center for Skeletal Stem Cells, State Key Laboratory of Cellular Stress Biology, School of Medicine, Faculty of Medicine and Life Sciences, Xiamen, China (GRID:grid.12955.3a) (ISNI:0000 0001 2264 7233) 
700 1 |a Chen, Wei  |u Hebei Medical University Third Hospital, Department of Orthopedic Surgery, Shijiazhuang, China (GRID:grid.256883.2) (ISNI:0000 0004 1760 8442); Key Laboratory of Biomechanics of Hebei Province, Shijiazhuang, China (GRID:grid.452209.8) (ISNI:0000 0004 1799 0194); NHC Key Laboratory of Intelligent Orthopedic Equipment, Shijiazhuang, China (GRID:grid.452209.8) 
773 0 |t Bone Research  |g vol. 13, no. 1 (2025), p. 54 
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