Single-cell analysis of ecto-mesenchymal cells derived from the neural crest reveals the regulatory role of P75NTR in odontogenic and osteogenic differentiation
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| izdano v: | BMC Oral Health vol. 25 (2025), p. 1-13 |
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| Drugi avtorji: | , , , , |
| Izdano: |
Springer Nature B.V.
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| Online dostop: | Citation/Abstract Full Text Full Text - PDF |
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| 001 | 3227646143 | ||
| 003 | UK-CbPIL | ||
| 022 | |a 1472-6831 | ||
| 024 | 7 | |a 10.1186/s12903-025-06399-z |2 doi | |
| 035 | |a 3227646143 | ||
| 045 | 2 | |b d20250101 |b d20251231 | |
| 084 | |a 58473 |2 nlm | ||
| 100 | 1 | |a Li, Feng | |
| 245 | 1 | |a Single-cell analysis of ecto-mesenchymal cells derived from the neural crest reveals the regulatory role of P75NTR in odontogenic and osteogenic differentiation | |
| 260 | |b Springer Nature B.V. |c 2025 | ||
| 513 | |a Journal Article | ||
| 520 | 3 | |a BackgroundThe aim to this study was to explore the effect of p75NTR on the mineralization and development of maxillofacial processes. Moreover, we tried to elaborate the potential mechanism of p75NTR’s effect on the odontogenic or osteogenic differentiation ability of neural crest cells (s).MethodsWe used gene p75NTR knockout (p75NTR−/−) mice and wildtype (p75NTR+/+) mice embryos as a model. And We isolated p75NTR−/− and p75NTR+/+ ectodermal mesenchymal stem cells (EMSCs) from the embryonic maxillofacial process of the same pregnant p75NTR+/− mice. The potential mechanism of p75NTR’s effect on the odontogenic or osteogenic differentiation ability of EMSCs was analyzed by single cell RNA sequence(scRNA-seq). The results were further verified by mineralize induction and wound-healing Assay.ResultsWe found the deletion of p75NTR could impact the communications between different cell types, influence differentiation and suppress odontogenesis via regulation networks of cytokines that associating with migration, proliferation and differentiation. The volcano plot characterized the distribution of differentially expressed genes (DEGs) in the selection at threshold level. p75NTR deletion suppressed EMSCs migration, proliferation and odonto/osteogenic differentiation in vitro.ConclusionsThis study provides a comprehensive analysis of the cellular fate of EMSCs in the maxillofacial process. p75NTR plays a crucial role in initiating hard tissue development and regulates the odontogenic and osteogenic differentiation of EMSCs during embryonic development. | |
| 651 | 4 | |a United States--US | |
| 651 | 4 | |a Germany | |
| 651 | 4 | |a China | |
| 651 | 4 | |a Japan | |
| 653 | |a Embryo cells | ||
| 653 | |a Odontogenesis | ||
| 653 | |a Embryogenesis | ||
| 653 | |a Mesenchymal stem cells | ||
| 653 | |a Data processing | ||
| 653 | |a Cell differentiation | ||
| 653 | |a Nucleotide sequence | ||
| 653 | |a Penicillin | ||
| 653 | |a Genes | ||
| 653 | |a Neural crest | ||
| 653 | |a Dental pulp | ||
| 653 | |a Signal transduction | ||
| 653 | |a Phosphatase | ||
| 653 | |a Mesenchyme | ||
| 653 | |a Maxillofacial | ||
| 653 | |a Medical research | ||
| 653 | |a Abdominal surgery | ||
| 653 | |a Mineralization | ||
| 653 | |a Stem cells | ||
| 653 | |a Wound healing | ||
| 653 | |a Hominids | ||
| 653 | |a Oral hygiene | ||
| 700 | 1 | |a Zhang, Yanyan | |
| 700 | 1 | |a Wang, Keyu | |
| 700 | 1 | |a Huang, Yanda | |
| 700 | 1 | |a Yuan, Qin | |
| 700 | 1 | |a Nie, Xin | |
| 773 | 0 | |t BMC Oral Health |g vol. 25 (2025), p. 1-13 | |
| 786 | 0 | |d ProQuest |t Health & Medical Collection | |
| 856 | 4 | 1 | |3 Citation/Abstract |u https://www.proquest.com/docview/3227646143/abstract/embedded/H09TXR3UUZB2ISDL?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text |u https://www.proquest.com/docview/3227646143/fulltext/embedded/H09TXR3UUZB2ISDL?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text - PDF |u https://www.proquest.com/docview/3227646143/fulltextPDF/embedded/H09TXR3UUZB2ISDL?source=fedsrch |