Small Things that Make a Big Difference: Single-Cell Transcriptomic of Nanociliates Reveals Genes Potentially Involved in Mixotrophy
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| Опубликовано в:: | Microbial Ecology vol. 88, no. 1 (Dec 2025), p. 72 |
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| Главный автор: | |
| Другие авторы: | , , |
| Опубликовано: |
Springer Nature B.V.
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| Online-ссылка: | Citation/Abstract Full Text Full Text - PDF |
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| 045 | 2 | |b d20251201 |b d20251231 | |
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| 100 | 1 | |a Romano, Filomena |u Hellenic Centre for Marine Research, Institute of Oceanography, Heraklion, Greece (GRID:grid.410335.0) (ISNI:0000 0001 2288 7106) | |
| 245 | 1 | |a Small Things that Make a Big Difference: Single-Cell Transcriptomic of Nanociliates Reveals Genes Potentially Involved in Mixotrophy | |
| 260 | |b Springer Nature B.V. |c Dec 2025 | ||
| 513 | |a Journal Article | ||
| 520 | 3 | |a Nanociliates play an important role in the microbial food web of oligotrophic marine systems as grazers of picoplankton on one side, and as prey for microplankton, on the other. However, knowledge on their taxonomy, phylogeny, and trophic strategies is very limited, as well as their potential role as mixotrophs. In the present study, we investigated the transcriptomes of five marine planktonic nanociliates isolated from the Eastern Mediterranean Sea. Our aim was the following: (i) to characterize the phylogenetic placement of these cells using concatenated phylotranscriptomic and (ii) to identify genes potentially involved in mixotrophy by focusing on both photosynthesis and digestion-related genes (phagosome, lysosome). Phylogenetic reconstruction revealed that two cells clustered with Tintinnida, while the other three clustered with Oligotrichida. Reciprocal best hits (RHBs) BlastP analysis indicated the presence of genes related to photosynthesis across all the transcriptomes, while the detection of genes associated with phagosome, lysosome, and generic metabolic pathways provided a more informative insight into the mechanism of mixotrophy. These findings suggest that photosynthesis-related genes alone may not be sufficient indicators of mixotrophic potential in nanociliates and highlight the importance of considering additional cellular pathways involved in phagotrophy. Moreover, these transcriptomes will help to establish a basis for the evaluation of differential gene expression in Oligotrichida, Choreotrichida, and Tintinnida, and a step stone for mixotrophic investigation. | |
| 651 | 4 | |a United States--US | |
| 651 | 4 | |a Mediterranean Sea | |
| 653 | |a Transcriptomes | ||
| 653 | |a Lysosomes | ||
| 653 | |a Software | ||
| 653 | |a Picoplankton | ||
| 653 | |a Phylogenetics | ||
| 653 | |a Food webs | ||
| 653 | |a Phylogeny | ||
| 653 | |a Genes | ||
| 653 | |a Gene expression | ||
| 653 | |a Photosynthesis | ||
| 653 | |a Chloroplasts | ||
| 653 | |a Taxonomy | ||
| 653 | |a Metabolism | ||
| 653 | |a Transcriptomics | ||
| 653 | |a Genomics | ||
| 653 | |a Cell cycle | ||
| 653 | |a Marine systems | ||
| 653 | |a Food chains | ||
| 653 | |a Microorganisms | ||
| 653 | |a Metabolic pathways | ||
| 653 | |a Mixotrophy | ||
| 653 | |a Protozoa | ||
| 653 | |a Tintinnida | ||
| 653 | |a Oligotrichida | ||
| 653 | |a Environmental | ||
| 700 | 1 | |a John, Uwe |u Alfred Wegener Institute (AWI), Helmholtz Centre for Polar and Marine Research, Department of Ecological Chemistry, Bremerhaven, Germany (GRID:grid.10894.34) (ISNI:0000 0001 1033 7684); Helmholtz Institute for Functional Marine Biodiversity at the University of Oldenburg (HIFMB), Oldenburg, Germany (GRID:grid.511218.e) | |
| 700 | 1 | |a Laval-Peuto, Michele |u Université de NICE, Faculté Des Sciences Nice-Valrose, Valrose, France (GRID:grid.460782.f) (ISNI:0000 0004 4910 6551); Le Colle Sur Loup, France (GRID:grid.460782.f) | |
| 700 | 1 | |a Pitta, Paraskevi |u Hellenic Centre for Marine Research, Institute of Oceanography, Heraklion, Greece (GRID:grid.410335.0) (ISNI:0000 0001 2288 7106) | |
| 773 | 0 | |t Microbial Ecology |g vol. 88, no. 1 (Dec 2025), p. 72 | |
| 786 | 0 | |d ProQuest |t Health & Medical Collection | |
| 856 | 4 | 1 | |3 Citation/Abstract |u https://www.proquest.com/docview/3228585044/abstract/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |
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