Dissecting cell-free DNA fragmentation variation in tumors using cell line-derived xenograft mouse
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| Publicado en: | PLoS One vol. 20, no. 7 (Jul 2025), p. e0327483 |
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| Otros Autores: | , , , , |
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Public Library of Science
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| Acceso en línea: | Citation/Abstract Full Text Full Text - PDF |
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| 001 | 3230449901 | ||
| 003 | UK-CbPIL | ||
| 022 | |a 1932-6203 | ||
| 024 | 7 | |a 10.1371/journal.pone.0327483 |2 doi | |
| 035 | |a 3230449901 | ||
| 045 | 2 | |b d20250701 |b d20250731 | |
| 084 | |a 174835 |2 nlm | ||
| 100 | 1 | |a Fu, Ruiqing | |
| 245 | 1 | |a Dissecting cell-free DNA fragmentation variation in tumors using cell line-derived xenograft mouse | |
| 260 | |b Public Library of Science |c Jul 2025 | ||
| 513 | |a Journal Article | ||
| 520 | 3 | |a Cell-free DNA (cfDNA) is increasingly studied for its diverse applications in non-invasive detection. Non-randomly cleaved by nucleases and released into the bloodstream, cfDNA exhibits a variety of intrinsic fragmentation patterns indicative of cell status. Particularly, these fragmentation patterns have recently been demonstrated to be effective in predicting cancer and its tissue-of-origin, owing to increased variation of fragmentation features observed in tumor patients. However, there remains a lack of detailed exploration of altered cfDNA fragmentation profiles in tumors, which consist of a mixture of both non-tumor cfDNA and circulating tumor DNA (ctDNA). Hence, we leveraged the human tumor cell line-derived xenograft (CDX) mouse model, where different tumor cell lines were implanted into different anatomical sites, to isolate pure ctDNA and separately investigate the fragment properties of CDX-cfDNA and ctDNA. We found an enrichment of short cfDNA fragments in both CDX-cfDNA and ctDNA compared to normal plasma cfDNA, with more elevated short fragments in ctDNA. Moreover, the CDX-cfDNA fragmentation features distinguished between CDX models of different tumor cell lines, while the ctDNA fragmentation features conversely discriminate between CDX models of different anatomical sites. The results suggested that both non-tumor cfDNA and ctDNA contribute to the increased variation observed in tumors, and that cfDNA fragmentation may be highly variable and susceptible to regulations by both original cells and cells within the local niche. | |
| 653 | |a Cancer | ||
| 653 | |a DNA methylation | ||
| 653 | |a Tumors | ||
| 653 | |a Plasma | ||
| 653 | |a Tumorigenesis | ||
| 653 | |a Fragments | ||
| 653 | |a Mutation | ||
| 653 | |a Cell culture | ||
| 653 | |a Variation | ||
| 653 | |a Deoxyribonucleic acid--DNA | ||
| 653 | |a Xenotransplantation | ||
| 653 | |a Tumor cell lines | ||
| 653 | |a Genomes | ||
| 653 | |a Animal models | ||
| 653 | |a DNA fragmentation | ||
| 653 | |a Fragmentation | ||
| 653 | |a Xenografts | ||
| 653 | |a Environmental | ||
| 700 | 1 | |a He, Amy Su | |
| 700 | 1 | |a Zhao, Yi | |
| 700 | 1 | |a Tian, Yafei | |
| 700 | 1 | |a Chen, Hongyan | |
| 700 | 1 | |a Lu, Daru | |
| 773 | 0 | |t PLoS One |g vol. 20, no. 7 (Jul 2025), p. e0327483 | |
| 786 | 0 | |d ProQuest |t Health & Medical Collection | |
| 856 | 4 | 1 | |3 Citation/Abstract |u https://www.proquest.com/docview/3230449901/abstract/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text |u https://www.proquest.com/docview/3230449901/fulltext/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text - PDF |u https://www.proquest.com/docview/3230449901/fulltextPDF/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |