A Meta-Review of Spatial Transcriptomics Analysis Software

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Veröffentlicht in:Cells vol. 14, no. 14 (2025), p. 1060-1079
1. Verfasser: Gillespie, Jessica
Weitere Verfasser: Pietrzak Maciej, Min-Ae, Song, Chung, Dongjun
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MDPI AG
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100 1 |a Gillespie, Jessica  |u Department of Biomedical Informatics, The Ohio State University, Columbus, OH 43210, USA; jessica.gillespie@osumc.edu (J.G.); maciej.pietrzak@osumc.edu (M.P.) 
245 1 |a A Meta-Review of Spatial Transcriptomics Analysis Software 
260 |b MDPI AG  |c 2025 
513 |a Journal Article 
520 3 |a Spatial transcriptomics combines gene expression data with spatial coordinates to allow for the discovery of detailed RNA localization, study development, investigating the tumor microenvironment, and creating a tissue atlas. A large range of spatial transcriptomics software is available, with little information on which may be better suited for particular datasets or computing environments. A review was conducted to detail the useful metrics when choosing appropriate software for spatial transcriptomics analysis. Specifically, the results from benchmarking studies that compared software across four key areas of spatial transcriptomics analysis (tissue architecture identification, spatially variable gene discovery, cell–cell communication analysis, and deconvolution) were assimilated into a single review that can serve as guidance when choosing potential spatial transcriptomics analysis software. 
653 |a Accuracy 
653 |a Software packages 
653 |a Tumor microenvironment 
653 |a Gene expression 
653 |a Cell interactions 
653 |a Histology 
653 |a Datasets 
653 |a Transcriptomics 
653 |a Localization 
653 |a Communication 
653 |a Software 
653 |a Benchmarks 
700 1 |a Pietrzak Maciej  |u Department of Biomedical Informatics, The Ohio State University, Columbus, OH 43210, USA; jessica.gillespie@osumc.edu (J.G.); maciej.pietrzak@osumc.edu (M.P.) 
700 1 |a Min-Ae, Song  |u Division of Environmental Health Science, College of Public Health, The Ohio State University, Columbus, OH 43210, USA; song.991@osu.edu 
700 1 |a Chung, Dongjun  |u Department of Biomedical Informatics, The Ohio State University, Columbus, OH 43210, USA; jessica.gillespie@osumc.edu (J.G.); maciej.pietrzak@osumc.edu (M.P.) 
773 0 |t Cells  |g vol. 14, no. 14 (2025), p. 1060-1079 
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