L‐quebrachitol Modulates the Anti‐convulsant Effects of Carbamazepine Possibly Through Voltage‐gated Sodium Channel Blocking Mechanism in Chicks: In Vivo and In Silico Studies

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Publicado en:Brain and Behavior vol. 15, no. 7 (Jul 1, 2025)
Autor principal: Asrafi, Asifa
Otros Autores: Aslam, Mohammad, Alkhathami, Ali G., Hossain, Md. Sakib, Rakib, Imam Hossen, Al Hasan, Md. Sakib, Nun, Feroz Khan, Amin, Md. Faisal, Islam, Muhammad Torequl
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022 |a 2162-3279 
024 7 |a 10.1002/brb3.70675  |2 doi 
035 |a 3233904571 
045 0 |b d20250701 
084 |a 162435  |2 nlm 
100 1 |a Asrafi, Asifa  |u Department of Biochemistry and Molecular Biology, Gopalganj Science and Technology University, Gopalganj, Bangladesh 
245 1 |a L‐quebrachitol Modulates the Anti‐convulsant Effects of Carbamazepine Possibly Through Voltage‐gated Sodium Channel Blocking Mechanism in Chicks: In Vivo and In Silico Studies 
260 |b John Wiley & Sons, Inc.  |c Jul 1, 2025 
513 |a Journal Article 
520 3 |a ABSTRACT Introduction L‐Quebrachitol (LQB), a naturally occurring bioactive compound, exhibits anti‐inflammatory, anti‐oxidant, anti‐cancer, and anti‐diabetic properties. However, its therapeutic potential in convulsant management remains largely unexplored. The objective of this study was to investigate the anticonvulsant effects of LQB in an In Vivo model and to examine its molecular interactions via In Silico docking simulations. Methods In the In Vivo study, pentylenetetrazol (PTZ) was administered intraperitoneally (i.p.) at 80&#xa0;mg/kg to induce convulsions, and the test animals were treated orally with three doses of LQB (1, 5, and 10&#xa0;mg/kg), with carbamazepine (CBZ) at 80&#xa0;mg/kg as a standard drug. Results The results indicated that LQB at all tested doses significantly (p < 0.05) prolonged seizure latency and decreased convulsion frequency, with the 10&#xa0;mg/kg dose showing the most significant effects. Furthermore, the combination of LQB (10&#xa0;mg/kg) and CBZ (80&#xa0;mg/kg) resulted in a synergistic increase in anticonvulsant activity. In the In Silico study, molecular docking analysis revealed that both LQB and CBZ interacted with the voltage‐gated sodium channel (VGSC), a key receptor involved in convulsions, with LQB demonstrating a binding affinity (BA) of −5.4&#xa0;kcal/mol, which was moderate compared to CBZ's BA. Conclusion LQB showed potential&#xa0;anti‐convulsant activity in PTZ‐induced convulsion animals, possibly through blocking sodium channel receptors. Further studies are needed to clarify its mechanisms and clinical potential in convulsion treatment. 
651 4 |a Bangladesh 
653 |a Convulsions & seizures 
653 |a Anticonvulsants 
653 |a Epilepsy 
653 |a Animals 
653 |a Sodium 
653 |a Free radicals 
653 |a Natural products 
653 |a Mutation 
653 |a Drug dosages 
653 |a Oxidative stress 
700 1 |a Aslam, Mohammad  |u Department of Biochemistry and Molecular Biology, Gopalganj Science and Technology University, Gopalganj, Bangladesh 
700 1 |a Alkhathami, Ali G.  |u Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, Abha, Saudi Arabia 
700 1 |a Hossain, Md. Sakib  |u Department of Pharmacy, Gopalganj Science and Technology University, Gopalganj, Bangladesh 
700 1 |a Rakib, Imam Hossen  |u Department of Pharmacy, Gopalganj Science and Technology University, Gopalganj, Bangladesh 
700 1 |a Al Hasan, Md. Sakib  |u Department of Pharmacy, Gopalganj Science and Technology University, Gopalganj, Bangladesh 
700 1 |a Nun, Feroz Khan  |u Department of Pharmacy, Gopalganj Science and Technology University, Gopalganj, Bangladesh 
700 1 |a Amin, Md. Faisal  |u School of Integrative Biological and Chemical Sciences, The University of Texas Rio Grande Valley, Texas, USA 
700 1 |a Islam, Muhammad Torequl  |u Department of Pharmacy, Gopalganj Science and Technology University, Gopalganj, Bangladesh 
773 0 |t Brain and Behavior  |g vol. 15, no. 7 (Jul 1, 2025) 
786 0 |d ProQuest  |t Health & Medical Collection 
856 4 1 |3 Citation/Abstract  |u https://www.proquest.com/docview/3233904571/abstract/embedded/J7RWLIQ9I3C9JK51?source=fedsrch 
856 4 0 |3 Full Text  |u https://www.proquest.com/docview/3233904571/fulltext/embedded/J7RWLIQ9I3C9JK51?source=fedsrch 
856 4 0 |3 Full Text - PDF  |u https://www.proquest.com/docview/3233904571/fulltextPDF/embedded/J7RWLIQ9I3C9JK51?source=fedsrch