Molecular characterization of Echinocandin resistance and the CHS3-mediated adaptive response in Candida glabrata bloodstream infections in Eastern China
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| Vydáno v: | BMC Microbiology vol. 25 (2025), p. 1-12 |
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| Další autoři: | , , , , , , |
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Springer Nature B.V.
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MARC
| LEADER | 00000nab a2200000uu 4500 | ||
|---|---|---|---|
| 001 | 3247129302 | ||
| 003 | UK-CbPIL | ||
| 022 | |a 1471-2180 | ||
| 024 | 7 | |a 10.1186/s12866-025-04155-5 |2 doi | |
| 035 | |a 3247129302 | ||
| 045 | 2 | |b d20250101 |b d20251231 | |
| 084 | |a 58455 |2 nlm | ||
| 100 | 1 | |a Cong, Peishan | |
| 245 | 1 | |a Molecular characterization of Echinocandin resistance and the <i>CHS3</i>-mediated adaptive response in <i>Candida glabrata</i> bloodstream infections in Eastern China | |
| 260 | |b Springer Nature B.V. |c 2025 | ||
| 513 | |a Journal Article | ||
| 520 | 3 | |a BackgroundCandidemia caused by Candida glabrata is a serious fungal infection, and rising echinocandin resistance presents a significant clinical challenge. Understanding the drug susceptibility profiles, molecular epidemiology, and mechanisms underlying adaptive echinocandin resistance in C. glabrata is crucial.ResultsA total of 106 C. glabrata strains were isolated from blood cultures of 103 candidemia patients across three medical centers in eastern China. Transcriptome sequencing and whole-genome sequence analysis were used to explore the genomic characteristics of echinocandin-resistant strains. Multi-locus sequence typing (MLST) categorized the isolates into 11 sequence types (STs), with ST7 being the most prevalent (67.9%). Drug susceptibility testing revealed a fluconazole resistance rate of 21.7%, while non-wild-type rates for voriconazole, itraconazole, and posaconazole were 23.6%, 7.5%, and 6.6%, respectively. One isolate (Q2-2) was resistant to all three echinocandins. Two isolates were resistant to micafungin and anidulafungin, respectively. Compared to the echinocandin-sensitive strains, the expression of the Chitin synthetase 3 (CHS3) gene was significantly upregulated in echinocandin-resistant strains. Functional analysis of a CHS3-overexpressing strain (ATCC2001-CHS3-OE), generated through homologous recombination, confirmed echinocandin resistance. Conversely, a CHS3 knockout strain (Q2-2-CHS3Δ) exhibited susceptibility to echinocandins.ConclusionsOur findings suggest that CHS3 plays a critical compensatory role in echinocandin resistance in C. glabrata, offering a promising target for developing future antifungal strategies. | |
| 651 | 4 | |a United States--US | |
| 651 | 4 | |a China | |
| 653 | |a Infections | ||
| 653 | |a Transcriptomes | ||
| 653 | |a Software | ||
| 653 | |a Diabetes | ||
| 653 | |a Fungicides | ||
| 653 | |a Ventilators | ||
| 653 | |a Sequence analysis | ||
| 653 | |a Epidemiology | ||
| 653 | |a Mutation | ||
| 653 | |a Micafungin | ||
| 653 | |a Voriconazole | ||
| 653 | |a Drugs | ||
| 653 | |a Genomes | ||
| 653 | |a Nucleotide sequence | ||
| 653 | |a Antifungal agents | ||
| 653 | |a Genes | ||
| 653 | |a Candidemia | ||
| 653 | |a Patients | ||
| 653 | |a Health care facilities | ||
| 653 | |a Blood diseases | ||
| 653 | |a Itraconazole | ||
| 653 | |a Gene sequencing | ||
| 653 | |a Fluconazole | ||
| 653 | |a Antibiotics | ||
| 653 | |a DNA polymerase | ||
| 653 | |a Homologous recombination | ||
| 653 | |a Chitin | ||
| 653 | |a Functional analysis | ||
| 653 | |a Posaconazole | ||
| 653 | |a Genomic analysis | ||
| 653 | |a Echinocandins | ||
| 653 | |a Multilocus sequence typing | ||
| 653 | |a Social | ||
| 653 | |a Candida glabrata | ||
| 700 | 1 | |a Wang, Biao | |
| 700 | 1 | |a He, Hong | |
| 700 | 1 | |a Li, Xiaoying | |
| 700 | 1 | |a Peng, Lijing | |
| 700 | 1 | |a Liu, Ji | |
| 700 | 1 | |a Guo, Hui | |
| 700 | 1 | |a Sun, Guirong | |
| 773 | 0 | |t BMC Microbiology |g vol. 25 (2025), p. 1-12 | |
| 786 | 0 | |d ProQuest |t Health & Medical Collection | |
| 856 | 4 | 1 | |3 Citation/Abstract |u https://www.proquest.com/docview/3247129302/abstract/embedded/75I98GEZK8WCJMPQ?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text |u https://www.proquest.com/docview/3247129302/fulltext/embedded/75I98GEZK8WCJMPQ?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text - PDF |u https://www.proquest.com/docview/3247129302/fulltextPDF/embedded/75I98GEZK8WCJMPQ?source=fedsrch |