Identification of a novel aminoglycoside nucleotidyltransferase ANT(3″)-Ic from Citrobacter telavivum S24

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Vydáno v:BMC Microbiology vol. 25 (2025), p. 1-13
Hlavní autor: Lu, Junwan
Další autoři: Zhu, Mei, Ye, Jingzeng, Xu, Leyao, Song, Chunhan, Li, Kewei, Bao, Qiyu, Lin, Xi
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Springer Nature B.V.
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022 |a 1471-2180 
024 7 |a 10.1186/s12866-025-04282-z  |2 doi 
035 |a 3247132672 
045 2 |b d20250101  |b d20251231 
084 |a 58455  |2 nlm 
100 1 |a Lu, Junwan 
245 1 |a Identification of a novel aminoglycoside nucleotidyltransferase ANT(3″)-Ic from <i>Citrobacter telavivum</i> S24 
260 |b Springer Nature B.V.  |c 2025 
513 |a Journal Article 
520 3 |a Understanding the antibiotic resistance profile of the emerging pathogen Citrobacter telavivum S24 is important for clinical care, surveillance, and research. C. telavivum S24, a strain that exhibits resistance to both streptomycin and spectinomycin, was isolated from the soil at a broiler chicken farm. The strain was identified by whole-genome sequencing and average nucleotide identity analyses. Annotation of the resistance genes revealed that a novel aminoglycoside resistance gene, designated ant(3″)-Ic, is located on the chromosome of C. telavivum S24. This gene exhibits 52% similarity with the previously functionally characterized resistance gene aadA23. It distinguishes itself from ant(3″)-Ia and ant(3″)-Ib genes, and represents an independent branch. The ant(3″)-Ic gene is recognized as an inherent resistance gene of C. telavivum within a conserved genetic environment. The product of the ant(3″)-Ic gene is the aminoglycoside nucleotidyltransferase ANT(3″)-Ic, which has a theoretical pI of 5.05 and a molecular mass of 29 kDa. ANT(3”)-Ic inactivates streptomycin and spectinomycin with the Km of 46.13 and 115.90 µM, respectively. The discovery of the novel antibiotic resistance gene ant(3″)-Ic and the functional characterization of ANT(3”)-Ic will facilitate more targeted surveillance and treatment strategies for preventing and controlling zoonotic pathogens of zoonotic diseases. 
651 4 |a Beijing China 
651 4 |a United States--US 
651 4 |a Shanghai China 
651 4 |a China 
651 4 |a California 
651 4 |a Japan 
653 |a Infections 
653 |a Antibiotic resistance 
653 |a Surveillance 
653 |a Phylogenetics 
653 |a Genes 
653 |a Streptomycin 
653 |a Zoonoses 
653 |a Genomes 
653 |a Antibiotics 
653 |a E coli 
653 |a Annotations 
653 |a Drug resistance 
653 |a Proteins 
653 |a Pathogens 
653 |a Plasmids 
653 |a Spectinomycin 
653 |a Nucleotides 
653 |a Citrobacter 
653 |a Gene sequencing 
653 |a Aminoglycosides 
653 |a Cloning 
653 |a Aminoglycoside antibiotics 
653 |a Glucose 
653 |a Antimicrobial agents 
653 |a Whole genome sequencing 
653 |a Poultry farming 
653 |a Enzymes 
653 |a Poultry 
653 |a Environmental 
700 1 |a Zhu, Mei 
700 1 |a Ye, Jingzeng 
700 1 |a Xu, Leyao 
700 1 |a Song, Chunhan 
700 1 |a Li, Kewei 
700 1 |a Bao, Qiyu 
700 1 |a Lin, Xi 
773 0 |t BMC Microbiology  |g vol. 25 (2025), p. 1-13 
786 0 |d ProQuest  |t Health & Medical Collection 
856 4 1 |3 Citation/Abstract  |u https://www.proquest.com/docview/3247132672/abstract/embedded/J7RWLIQ9I3C9JK51?source=fedsrch 
856 4 0 |3 Full Text  |u https://www.proquest.com/docview/3247132672/fulltext/embedded/J7RWLIQ9I3C9JK51?source=fedsrch 
856 4 0 |3 Full Text - PDF  |u https://www.proquest.com/docview/3247132672/fulltextPDF/embedded/J7RWLIQ9I3C9JK51?source=fedsrch