Magnolia figo Extract Induces Enamel Shade Recovery and Inhibits Porphyromonas gingivalis Biofilm Formation: An In Vitro, Dual-Action Natural Therapeutic Approach

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Publicado en:International Journal of Molecular Sciences vol. 26, no. 17 (2025), p. 8157-8175
Autor principal: Chun-Sheng, Kuo
Otros Autores: Cheng-Wen, Lin, Yuan-Man, Hsu, Tsai Jen-Chieh, Dan-Jae, Lin
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MDPI AG
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022 |a 1422-0067 
024 7 |a 10.3390/ijms26178157  |2 doi 
035 |a 3249691930 
045 2 |b d20250901  |b d20250914 
084 |a 231645  |2 nlm 
100 1 |a Chun-Sheng, Kuo  |u PhD Program for Health Science and Industry, China Medical University, Taichung 404328, Taiwan; u110310201@cmu.edu.tw (C.-S.K.); cwlin@mail.cmu.edu.tw (C.-W.L.) 
245 1 |a <i>Magnolia figo</i> Extract Induces Enamel Shade Recovery and Inhibits <i>Porphyromonas gingivalis</i> Biofilm Formation: An In Vitro, Dual-Action Natural Therapeutic Approach 
260 |b MDPI AG  |c 2025 
513 |a Journal Article 
520 3 |a Dental enamel discoloration, extrinsic staining, and periodontal biofilms remain persistent challenges in oral health. This study explores the in vitro, dual-functional potential of Magnolia figo flower extract (FMO), a sesquiterpene-rich botanical active phytochemical ingredient (API), for aesthetic and antimicrobial oral applications. FTIR identified characteristic terpenoid and long-chain fatty acid functional groups, including β-elemene, γ-elemene, and caryophyllene oxide. Whitening efficacy on coffee-stained bovine enamel was quantified using CIELAB colorimetry. The 0.5% FMO treatment achieved ΔE* = 8.49, which was within the clinical perceptibility threshold and the optimal biocompatibility balance. SEM confirmed no demineralization on the enamel surface after immersion in 3.0% FMO for 12 h. Antimicrobial assays demonstrated inhibition of Porphyromonas gingivalis, with MIC and MBC values of 0.25% and 0.5%, respectively. Biofilm formation was reduced by over 50% at a 0.148% concentration. Cytocompatibility assays using HGF-1 cells with various concentrations of FMO showed reduced cell viability at higher concentrations. When exposed for 5 min (simulating daily oral care) or 2 h, 0.5% FMO exhibited greater biocompatibility with L929 cells compared to toothpaste and peroxide-based agents. These findings suggest that FMO may serve as a natural candidate for dual-function oral care; however, further in vivo and clinical investigations are needed to validate its potential use within oral care treatments. 
653 |a Pathogens 
653 |a Dental enamel 
653 |a Oral hygiene 
653 |a Spectrum analysis 
653 |a Biofilms 
653 |a Disease 
653 |a Phytochemicals 
653 |a Morphology 
653 |a Hydroxyapatite 
653 |a Scanning electron microscopy 
700 1 |a Cheng-Wen, Lin  |u PhD Program for Health Science and Industry, China Medical University, Taichung 404328, Taiwan; u110310201@cmu.edu.tw (C.-S.K.); cwlin@mail.cmu.edu.tw (C.-W.L.) 
700 1 |a Yuan-Man, Hsu  |u Department of Animal Science and Biotechnology, Tunghai University, Taichung 407224, Taiwan; yuanmh@thu.edu.tw 
700 1 |a Tsai Jen-Chieh  |u School of Pharmacy, China Medical University, Taichung 406040, Taiwan; jenchieh@mail.cmu.edu.tw 
700 1 |a Dan-Jae, Lin  |u School of Dentistry, College of Dentistry, China Medical University, Taichung 404328, Taiwan 
773 0 |t International Journal of Molecular Sciences  |g vol. 26, no. 17 (2025), p. 8157-8175 
786 0 |d ProQuest  |t Health & Medical Collection 
856 4 1 |3 Citation/Abstract  |u https://www.proquest.com/docview/3249691930/abstract/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch 
856 4 0 |3 Full Text + Graphics  |u https://www.proquest.com/docview/3249691930/fulltextwithgraphics/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch 
856 4 0 |3 Full Text - PDF  |u https://www.proquest.com/docview/3249691930/fulltextPDF/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch