Transcriptomic responses of lung mesenchymal cells during pneumonia
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| Veröffentlicht in: | JCI Insight vol. 10, no. 7 (Apr 2025) |
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American Society for Clinical Investigation
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| 022 | |a 2379-3708 | ||
| 024 | 7 | |a 10.1172/jci.insight.177084 |2 doi | |
| 035 | |a 3256005325 | ||
| 045 | 2 | |b d20250401 |b d20250430 | |
| 100 | 1 | |a Soucy, Alicia M | |
| 245 | 1 | |a Transcriptomic responses of lung mesenchymal cells during pneumonia | |
| 260 | |b American Society for Clinical Investigation |c Apr 2025 | ||
| 513 | |a Journal Article | ||
| 520 | 3 | |a The role of mesenchymal cells during respiratory infection is not well defined, including whether, which, and how the different types of mesenchymal cells respond. We collected all mesenchymal cells from lung single-cell suspensions of mice that were naive (after receiving only saline vehicle), pneumonic (after intratracheal instillation of pneumococcus 24 hours previously), or resolved from infection (after nonlethal pneumococcal infections 6 weeks previously) and performed single-cell RNA sequencing. Cells clustered into 5 well-separated groups based on their transcriptomes: matrix fibroblasts, myofibroblasts, pericytes, smooth muscle cells, and mesothelial cells. Fibroblasts were the most abundant and could be further segregated into Pdgfra+Npnt+Ces1d+Col13a1+ alveolar fibroblasts and Cd9+Pi16+Sca1+Col14a1+ adventitial fibroblasts. The cells from naive and resolved groups overlapped in dimension reduction plots, suggesting the mesenchymal cells returned to baseline transcriptomes after resolution. During pneumonia, all mesenchymal cells responded with altered transcriptomes, revealing a core response that had been conserved across cell types as well as distinct mesenchymal cell type–specific responses. The different subsets of fibroblasts induced similar gene sets, but the alveolar fibroblasts responded more strongly than the adventitial fibroblasts. These data demonstrated diverse and specialized immune activities of lung mesenchymal cells during pneumonia. | |
| 653 | |a Infections | ||
| 653 | |a Transcriptomes | ||
| 653 | |a Neutrophils | ||
| 653 | |a Pneumonia | ||
| 653 | |a Cell suspensions | ||
| 653 | |a Cytokines | ||
| 653 | |a Leukocytes | ||
| 653 | |a Trachea | ||
| 653 | |a Fibroblasts | ||
| 653 | |a Smooth muscle | ||
| 653 | |a Transcriptomics | ||
| 653 | |a Genomics | ||
| 653 | |a Alveoli | ||
| 653 | |a Genes | ||
| 653 | |a Respiratory tract infection | ||
| 653 | |a Lungs | ||
| 653 | |a Pattern recognition | ||
| 653 | |a Cells | ||
| 653 | |a Ataxin | ||
| 653 | |a Mesenchyme | ||
| 653 | |a Immune response | ||
| 653 | |a CD9 antigen | ||
| 653 | |a Pericytes | ||
| 700 | 1 | |a Brune, Jourdan E | |
| 700 | 1 | |a Jayaraman, Archana | |
| 700 | 1 | |a Shenoy, Anukul T | |
| 700 | 1 | |a Korkmaz, Filiz T | |
| 700 | 1 | |a Etesami, Neelou S | |
| 700 | 1 | |a Hiller, Bradley E | |
| 700 | 1 | |a Ian M.C. Martin | |
| 700 | 1 | |a Goltry, Wesley N | |
| 700 | 1 | |a Ha, Catherine T | |
| 700 | 1 | |a Crossland, Nicholas A | |
| 700 | 1 | |a Campbell, Joshua D | |
| 700 | 1 | |a Beach, Thomas G | |
| 700 | 1 | |a Traber, Katrina E | |
| 700 | 1 | |a Jones, Matthew R | |
| 700 | 1 | |a Quinton, Lee J | |
| 700 | 1 | |a Bosmann, Markus | |
| 700 | 1 | |a Frevert, Charles W | |
| 700 | 1 | |a Mizgerd, Joseph P | |
| 773 | 0 | |t JCI Insight |g vol. 10, no. 7 (Apr 2025) | |
| 786 | 0 | |d ProQuest |t Health & Medical Collection | |
| 856 | 4 | 1 | |3 Citation/Abstract |u https://www.proquest.com/docview/3256005325/abstract/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text |u https://www.proquest.com/docview/3256005325/fulltext/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text - PDF |u https://www.proquest.com/docview/3256005325/fulltextPDF/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |