Phytochemical profiling, HPLC analysis, and antimicrobial potential of Curio radicans (L. f.) P.V. Heath

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Publicat a:Scientific Reports (Nature Publisher Group) vol. 15, no. 1 (2025), p. 34753-34764
Autor principal: Naseer, Muhammad
Altres autors: Adil, Muhammad
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Nature Publishing Group
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100 1 |a Naseer, Muhammad  |u Department of Chemical and Life Sciences, Qurtuba University of Science and Information Technology, Peshawar, Pakistan (ROR: https://ror.org/04ke3vc41) (GRID: grid.444994.0) (ISNI: 0000 0004 0609 284X) 
245 1 |a Phytochemical profiling, HPLC analysis, and antimicrobial potential of <i>Curio radicans</i> (L. f.) P.V. Heath 
260 |b Nature Publishing Group  |c 2025 
513 |a Journal Article 
520 3 |a Medicinal plants are dynamic reservoirs of bioactive molecules, offering immense potential for novel therapeutic discoveries. This study explores the phytochemical and antimicrobial potential of Curio radicans through detailed qualitative, quantitative, and HPLC analysis. Phytochemical screening revealed the ethanolic extract to be particularly rich in alkaloids, flavonoids, terpenoids, saponins, tannins, lipids, and phenols, whereas the ethyl acetate extract showed higher concentrations of phenolics and flavonoids. Quantitative estimations confirmed that the ethanolic extract possessed significantly higher levels of alkaloids (7.76&#xa0;mg/g), flavonoids (7.60&#xa0;mg/g), and tannins (10.32&#xa0;mg/g) compared to the ethyl acetate extract (3.51, 1.33, and 2.56&#xa0;mg/g, respectively). HPLC profiling further supported these findings, identifying abundant phenolic acids and flavonoids with well-documented pharmacological relevance. The ethanolic extract contained catechin, fumaric acid, hydroxybenzoic acid, caffeic acid, and salicylic acid, while the ethyl acetate extract was enriched with vanillin, protocatechuic acid, ellagic acid, caffeic acid, and p-coumaric acid. Antimicrobial assays demonstrated remarkable dose-dependent inhibition against both Gram-positive and Gram-negative bacteria, with Escherichia coli being highly susceptible (17.40 ± 1.15&#xa0;mm). Fungal strains, particularly Aspergillus niger, also showed significant sensitivity (15.27 ± 0.39&#xa0;mm). Collectively, these results validate the ethnomedicinal importance of C. radicans and underscore its potential as a natural source of bioactive compounds for pharmaceutical development. 
651 4 |a Pakistan 
653 |a Flavonoids 
653 |a Alkaloids 
653 |a Phenols 
653 |a Bacteria 
653 |a Pathogens 
653 |a Phenolic acids 
653 |a Tannins 
653 |a Phytochemicals 
653 |a Medicinal plants 
653 |a Ellagic acid 
653 |a Sulfuric acid 
653 |a Water 
653 |a Gram-negative bacteria 
653 |a Gram-positive bacteria 
653 |a Chromatography 
653 |a Salicylic acid 
653 |a Oils & fats 
653 |a Drug resistance 
653 |a Ethanol 
653 |a Saponins 
653 |a Lipids 
653 |a Protocatechuic acid 
653 |a Catechin 
653 |a Caffeic acid 
653 |a Ethyl acetate 
653 |a Bioactive compounds 
653 |a p-Coumaric acid 
653 |a Antimicrobial agents 
653 |a p-Hydroxybenzoic acid 
653 |a Acetic acid 
653 |a E coli 
653 |a High-performance liquid chromatography 
653 |a Vanillin 
653 |a Fumaric acid 
653 |a Environmental 
700 1 |a Adil, Muhammad  |u Department of Chemical and Life Sciences, Qurtuba University of Science and Information Technology, Peshawar, Pakistan (ROR: https://ror.org/04ke3vc41) (GRID: grid.444994.0) (ISNI: 0000 0004 0609 284X) 
773 0 |t Scientific Reports (Nature Publisher Group)  |g vol. 15, no. 1 (2025), p. 34753-34764 
786 0 |d ProQuest  |t Science Database 
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