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Adaptive genomic plasticity in large-genome, broad-host–range vibrio phages

Gorde:
Argitaratua izan da:The ISME Journal vol. 19, no. 1 (Jan 2025)
Egile nagusia: Bernard, Charles
Beste egile batzuk: Labreuche, Yannick, Diarra, Carine, Daszkowski, Pauline, Cahier, Karine, Goudenège, David, Lamarche, Martin G, Whitfield, Gregory B, Lang, Manon, Valencia, Jeffrey, Groseille, Justine, Piel, Damien, Lee, Yan-Jiun, Weigele, Peter, Brun, Yves V, Rocha, Eduardo P C, Le Roux, Frédérique
Argitaratua:
Oxford University Press
Gaiak:
Phages
Receptors
Seawater
Shellfish
Marine ecosystems
Burst size
Oysters
Host range
Aquaculture
Shellfish farming
Genomics
Genomes
Recombination hot spots
Bacteria
Environmental
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Laburpena:The host range of a bacteriophage—the diversity of hosts it can infect—is central to understanding phage ecology and applications. Whereas most well-characterized phages have narrow host ranges, broad-host–range phages represent an intriguing component of marine ecosystems. The genetic and evolutionary mechanisms driving their generalism remain poorly understood. In this study, we analyzed Schizotequatroviruses and their Vibrio crassostreae hosts, collected from an oyster farm. Schizotequatroviruses exhibit broad host ranges, large genomes (~252 kbp) encoding 26 transfer ribonucleic acids, and conserved genomic organization interspersed with recombination hotspots. These recombination events, particularly in regions encoding receptor-binding proteins and antidefense systems, highlight their adaptability to host resistance. Some lineages demonstrated the ability of receptor-switching between OmpK and LamB. Despite their broad host range, Schizotequatroviruses were rare in the environment. Their scarcity could not be attributed to burst size, which was comparable to other phages in vitro, but may result from ecological constraints or fitness trade-offs, such as their preference for targeting generalist vibrios in seawater rather than the patho-phylotypes selected in oyster farms. Our findings clarify the genetic and ecological variables shaping Schizotequatrovirus generalism and provide a foundation for future phage applications in aquaculture and beyond.
ISSN:1751-7362
1751-7370
DOI:10.1093/ismejo/wraf063
Baliabidea:Health & Medical Collection