Transcriptomic insights into soybean seed deterioration in uncontrolled storage

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Bibliografiska uppgifter
I publikationen:	Journal of Seed Science vol. 47 (2025)
Huvudupphov:	João Matheus Kafer
Övriga upphov:	Mayla Daiane Corrêa Molinari, Henning, Fernando Augusto, Lucas Vinícius de Sousa Alcântara, Alexandre Lima Nepomuceno, Mertz-Henning, Liliane Márcia
Utgiven:	Abrates - Associação Brasileira de Tecnologia de Sementes
Ämnen:	Soybeans Thioredoxin Genes Cysteine synthase Ribonucleic acid > RNA Thioredoxin peroxidase Phosphoprotein phosphatase Environmental conditions High temperature Peroxidase Transcriptomics Protein phosphatase Protein folding Cultivars Plant hormones Carbohydrate metabolism Alanine-glyoxylate transaminase Seeds Carbohydrates Proteins Agricultural commodities Oxidative stress Gene expression Aldehydes Cytochrome P450 Cytochromes P450 Lipid metabolism Lipids Transcription factors Transaminase Aldehyde dehydrogenase Protein turnover Peroxiredoxin Alanine
Länkar:	Citation/Abstract Full text outside of ProQuest
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Beskrivning
Abstrakt:	Soybean, the main agricultural commodity of Brazil, is sensitive to deterioration during storage due to its high fatty acid content and fragile seed coat, especially under tropical conditions where high temperature and humidity accelerate this process. The objective of this study is to investigate the mechanisms that are altered during the storage of soybean seeds under uncontrolled environmental conditions. For this purpose, RNA-seq libraries were generated from freshly harvested soybean seeds (FHS) and seeds stored under uncontrolled conditions (UES). The total RNA from seeds of the cultivar BRS 413 was extracted and sequenced for gene expression analysis. Relative expression indicated 325 genes repressed in UES and 150 induced when comparing the genes in stored seeds to freshly harvested ones. Genes related to protein folding, carbohydrate metabolism, lipids, phytohormones, and transcription factors showed a significant reduction in their expression during the storage. Among the genes uniquely expressed in UES, alanine-glyoxylate transaminase (GGAT), cysteine synthase (CSase), aldehyde dehydrogenase (ALDH), thioredoxin peroxidase (Peroxiredoxin), cytochrome P450 and protein phosphatase 2C (PP2C) stood out for making part of protective mechanisms against oxidative stress in plants.
ISSN:	2317-1537 2317-1545 0101-3122 1806-9975
DOI:	10.1590/2317-1545v47289243
Källa:	Agriculture Science Database