Environmental community transcriptomics: strategies and struggles

I tiakina i:
Ngā taipitopito rārangi puna kōrero
I whakaputaina i:Briefings in Functional Genomics vol. 24 (2025)
Kaituhi matua: Mante, Jeanet
Ētahi atu kaituhi: Groover, Kyra E, Pullen, Randi M
I whakaputaina:
Oxford University Press
Ngā marau:
Urunga tuihono:Citation/Abstract
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Whakaahuatanga
Whakarāpopotonga:Transcriptomics is the study of RNA transcripts, the portion of the genome that is transcribed, in a specific cell, tissue, or organism. Transcriptomics provides insight into gene expression patterns, regulation, and the underlying mechanisms of cellular processes. Community transcriptomics takes this a step further by studying the RNA transcripts from environmental assemblies of organisms, with the intention of better understanding the interactions between members of the community. Community transcriptomics requires successful extraction of RNA from a diverse set of organisms and subsequent analysis via mapping those reads to a reference genome or de novo assembly of the reads. Both, extraction protocols and the analysis steps can pose hurdles for community transcriptomics. This review covers advances in transcriptomic techniques and assesses the viability of applying them to community transcriptomics.
ISSN:2041-2649
2041-2657
1473-9550
DOI:10.1093/bfgp/elae033
Puna:Health & Medical Collection