DRBD3 regulates long non-coding RNA abundance and cryptic splice site selection in trypanosomes
-д хадгалсан:
| -д хэвлэсэн: | Cellular and Molecular Life Sciences vol. 82, no. 1 (Dec 2025), p. 386 |
|---|---|
| Үндсэн зохиолч: | |
| Бусад зохиолчид: | , , , |
| Хэвлэсэн: |
Springer Nature B.V.
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| Нөхцлүүд: | |
| Онлайн хандалт: | Citation/Abstract Full Text Full Text - PDF |
| Шошгууд: |
Шошго байхгүй, Энэхүү баримтыг шошголох эхний хүн болох!
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MARC
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| 024 | 7 | |a 10.1007/s00018-025-05929-w |2 doi | |
| 035 | |a 3269454498 | ||
| 045 | 2 | |b d20251201 |b d20251231 | |
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| 100 | 1 | |a Ceballos-Pérez, Gloria |u Instituto de Parasitología y Biomedicina ‘‘López-Neyra’’, IPBLN-CSIC. Parque Tecnológico de Ciencias de la Salud, Armilla, Spain (GRID:grid.429021.c) (ISNI:0000 0004 1775 8774) | |
| 245 | 1 | |a DRBD3 regulates long non-coding RNA abundance and cryptic splice site selection in trypanosomes | |
| 260 | |b Springer Nature B.V. |c Dec 2025 | ||
| 513 | |a Journal Article | ||
| 520 | 3 | |a Trypanosomes are unicellular eukaryotes that rely heavily on post-transcriptional mechanisms to control gene expression. DRBD3 is an RNA-binding protein known to play important roles in mRNA processing, stability, transport and translation. It was found to associate with grumpy, a long non-coding RNA (lncRNA) recently characterized in Trypanosoma brucei. Here, we explore the role of DRBD3 in lncRNA metabolism and show that its depletion leads to the upregulation of a specific subset of approximately one hundred lncRNAs in both bloodstream and procyclic forms, likely through the activation of cryptic splice sites. The effect of DRBD3 depletion on lncRNA expression appears to be mostly indirect, and results from reduced levels of the poly(A) polymerase PAP1 following DRBD3 silencing. In addition to its impact on lncRNAs, DRBD3 loss also affects the processing of protein-coding genes, leading to alternative trans-splicing and protein truncation. Furthermore, we demonstrate that DRBD3 regulates the splicing of the newly identified intron in the transcript encoding the RNA-binding protein RBP20, and is important for maintaining the balance between trans- and cis-splicing. Our results position DRBD3 as a high-level regulatory factor that shapes the expression landscape of both coding and non-coding genes in trypanosomes. | |
| 653 | |a Plasmids | ||
| 653 | |a Proteins | ||
| 653 | |a Gene expression | ||
| 653 | |a Polynucleotide adenylyltransferase | ||
| 653 | |a Eukaryotes | ||
| 653 | |a Depletion | ||
| 653 | |a Genes | ||
| 653 | |a RNA-binding protein | ||
| 653 | |a Ribonucleic acid--RNA | ||
| 653 | |a Alternative splicing | ||
| 653 | |a RNA transport | ||
| 653 | |a mRNA stability | ||
| 653 | |a Protozoa | ||
| 653 | |a Post-transcription | ||
| 653 | |a Non-coding RNA | ||
| 653 | |a Metabolism | ||
| 653 | |a Site selection | ||
| 653 | |a mRNA processing | ||
| 653 | |a Environmental | ||
| 700 | 1 | |a Gómez-Liñán, Claudia |u Instituto de Parasitología y Biomedicina ‘‘López-Neyra’’, IPBLN-CSIC. Parque Tecnológico de Ciencias de la Salud, Armilla, Spain (GRID:grid.429021.c) (ISNI:0000 0004 1775 8774) | |
| 700 | 1 | |a Sánchez-Luque, Francisco J. |u Instituto de Parasitología y Biomedicina ‘‘López-Neyra’’, IPBLN-CSIC. Parque Tecnológico de Ciencias de la Salud, Armilla, Spain (GRID:grid.429021.c) (ISNI:0000 0004 1775 8774) | |
| 700 | 1 | |a Pérez-Victoria, José M. |u Instituto de Parasitología y Biomedicina ‘‘López-Neyra’’, IPBLN-CSIC. Parque Tecnológico de Ciencias de la Salud, Armilla, Spain (GRID:grid.429021.c) (ISNI:0000 0004 1775 8774) | |
| 700 | 1 | |a Estévez, Antonio M. |u Instituto de Parasitología y Biomedicina ‘‘López-Neyra’’, IPBLN-CSIC. Parque Tecnológico de Ciencias de la Salud, Armilla, Spain (GRID:grid.429021.c) (ISNI:0000 0004 1775 8774) | |
| 773 | 0 | |t Cellular and Molecular Life Sciences |g vol. 82, no. 1 (Dec 2025), p. 386 | |
| 786 | 0 | |d ProQuest |t Health & Medical Collection | |
| 856 | 4 | 1 | |3 Citation/Abstract |u https://www.proquest.com/docview/3269454498/abstract/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text |u https://www.proquest.com/docview/3269454498/fulltext/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text - PDF |u https://www.proquest.com/docview/3269454498/fulltextPDF/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |