morphoHeart : A quantitative tool for integrated 3D morphometric analyses of heart and ECM during embryonic development
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| Publicado en: | PLoS Biology vol. 23, no. 1 (Jan 2025), p. e3002995-e3003030 |
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| Otros Autores: | , |
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Public Library of Science
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| Acceso en línea: | Citation/Abstract Full Text Full Text - PDF |
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| 022 | |a 1544-9173 | ||
| 022 | |a 1545-7885 | ||
| 024 | 7 | |a 10.1371/journal.pbio.3002995 |2 doi | |
| 035 | |a 3270865324 | ||
| 045 | 2 | |b d20250101 |b d20250131 | |
| 084 | |a 174830 |2 nlm | ||
| 100 | 1 | |a Sánchez-Posada, Juliana | |
| 245 | 1 | |a <i>morphoHeart</i> : A quantitative tool for integrated 3D morphometric analyses of heart and ECM during embryonic development | |
| 260 | |b Public Library of Science |c Jan 2025 | ||
| 513 | |a Journal Article | ||
| 520 | 3 | |a Heart development involves the complex structural remodelling of a linear heart tube into an asymmetrically looped and ballooned organ. Previous studies have associated regional expansion of extracellular matrix (ECM) space with tissue morphogenesis during development. We have developed morphoHeart, a 3D tissue segmentation and morphometry software with a user-friendly graphical interface (GUI) that delivers the first integrated 3D visualisation and multiparametric analysis of both heart and ECM morphology in live embryos. morphoHeart reveals that the ECM undergoes regional dynamic expansion and reduction during cardiac development, concomitant with chamber-specific morphological maturation. We use morphoHeart to demonstrate that regionalised ECM expansion driven by the ECM crosslinker Hapln1a promotes atrial lumen expansion during heart development. Finally, morphoHeart’s GUI expands its use beyond that of cardiac tissue, allowing its segmentation and morphometric analysis tools to be applied to z-stack images of any fluorescently labelled tissue. | |
| 653 | |a Regional development | ||
| 653 | |a User interface | ||
| 653 | |a Embryos | ||
| 653 | |a Software | ||
| 653 | |a Morphometry | ||
| 653 | |a Embryonic growth stage | ||
| 653 | |a Hyaluronic acid | ||
| 653 | |a Morphogenesis | ||
| 653 | |a Heart | ||
| 653 | |a Morphology | ||
| 653 | |a Embryogenesis | ||
| 653 | |a Segmentation | ||
| 653 | |a Extracellular matrix | ||
| 653 | |a Graphical user interface | ||
| 653 | |a Environmental | ||
| 700 | 1 | |a Derrick, Christopher J | |
| 700 | 1 | |a Noël, Emily S | |
| 773 | 0 | |t PLoS Biology |g vol. 23, no. 1 (Jan 2025), p. e3002995-e3003030 | |
| 786 | 0 | |d ProQuest |t Health & Medical Collection | |
| 856 | 4 | 1 | |3 Citation/Abstract |u https://www.proquest.com/docview/3270865324/abstract/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text |u https://www.proquest.com/docview/3270865324/fulltext/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |
| 856 | 4 | 0 | |3 Full Text - PDF |u https://www.proquest.com/docview/3270865324/fulltextPDF/embedded/7BTGNMKEMPT1V9Z2?source=fedsrch |